Existence of β-methylnorleucine in recombinant hirudin produced by Escherichia coli

Journal of Biotechnology

Volumn 93, Issue 2, 2002, Pages 131-142

  Muramatsu, Ryo ^a   Negishi, Toru ^a   Mimoto, Tsutomu ^a   Miura, Akira ^a   Misawa, Satoru ^a   Hayashi, Hideya ^a  

^a JAPAN ENERGY CORPORATION   (Japan)

Author keywords

Methylnorleucine; CX 397; Edman degradation; Escherichia coli; Hirudin; Thrombin

Indexed keywords

BIOSYNTHESIS; DERIVATIVES; ENZYME INHIBITION; ESCHERICHIA COLI; GENES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MUTAGENESIS; RNA;

MODIFICATION PATHWAY; RETENTION TIMES;

BIOTECHNOLOGY;

2 AMINO 3 ETHYLVALERIC ACID; AMINO ACID DERIVATIVE; CX 397; HIRUDIN; ISOLEUCINE; ISOLEUCINE TRANSFER RNA LIGASE; THROMBIN INHIBITOR; UNCLASSIFIED DRUG;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG STRUCTURE; DRUG SYNTHESIS; ENTEROBACTERIACEAE; ESCHERICHIA COLI; METHYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION; SERRATIA MARCESCENS; STRUCTURE ANALYSIS;

AMINO ACIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; GENE EXPRESSION; HIRUDINS; ISOLEUCINE; METHYLATION; NORLEUCINE; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; RECOMBINANT PROTEINS; THROMBIN; TRYPSIN;

ENTEROBACTERIACEAE; ESCHERICHIA COLI; SERRATIA MARCESCENS;

EID: 0037074718     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(01)00396-0     Document Type: Article

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