Characterization of copper/zinc-superoxide dismutase from Pagrus major cDNA and enzyme stability

Journal of Agricultural and Food Chemistry

Volumn 50, Issue 4, 2002, Pages 784-789

  Ken, Chuian Fu ^a   Weng, De Feng ^a   Duan, Kow Jen ^b   Lin, Chi Tsai ^a  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

^b TATUNG UNIVERSITY   (Taiwan)

Author keywords

Escherichia coli; Expression; Pagrus major; PCR; pET 20b(+)

Indexed keywords

AMINO ACID; ASPARAGINE; CHYMOTRYPSIN; COMPLEMENTARY DNA; COPPER; COPPER ZINC SUPEROXIDE DISMUTASE; CYSTEINE DERIVATIVE; DIMER; HISTIDINE; IMIDAZOLE DERIVATIVE; NICKEL; NITRILOTRIACETIC ACID; SEPHAROSE; SUPEROXIDE DISMUTASE; TRYPSIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; COMPARATIVE STUDY; COMPUTER ANALYSIS; DIMERIZATION; DISULFIDE BOND; DNA DETERMINATION; DNA SEQUENCE; DNA VECTOR; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; EXPRESSION VECTOR; FISH; GENETICS; HUMAN; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PAGRUS MAJOR; PH MEASUREMENT; POLYMERASE CHAIN REACTION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; RESIDUE ANALYSIS; SEQUENCE ALIGNMENT; GENE VECTOR;

ANIMALIA; ESCHERICHIA COLI; PAGRUS; PAGRUS MAJOR; VECTORS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ENZYME STABILITY; ESCHERICHIA COLI; FISHES; GENETIC VECTORS; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SUPEROXIDE DISMUTASE;

EID: 0037070034     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf0111559     Document Type: Article

References (22)

1. Superoxide dismutase from Bacillus stearothermophilus. Complete amino acid sequence of a manganese enzyme
2. Superoxide dismutases
3. Structural comparisons of superoxide dismutases
4. The role of biotransformation in the toxicity of chemicals
5. Towards a specific index of impact by organic pollution for marine invertbrates
6. Prooxidant and antioxidant mechanisms in aquatic organisms
7. Biochemistry of oxidative stress
8. Biochemical effects of environmental pollution in fishes from Spanish South-Atlantic littoral
9. Metal, mutagenicity, and biochemical studies on bivalve molluscs from Spanish coasts
10. Purification of Cu, Zn-superoxide dismutase isoenzymes from fish liver: Appearance of new isoforms as a consequence of pollution
11. Combined effect of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (Cyprinus carpio)
12. Substitution of arginine for lysine 134 alters electrostatic parameters of the active site in shark Cu/Zn superoxide dismutase
13. Molecular cloning of a cDNA coding for a Cu/Zn-superoxide dismutase from zebrafish and overexpression in Escherichia coli
14. Molecular cloning, characterization and expression of a cDNA coding opper/zinc-superoxide dismutase from black porgy
15. Hybridization with radioactive probes
16. Improved assays and an assay applicable to acrylamide gel
17. Selection of AUG initiation codons differs in plants and animals
18. Unique molecular properties of superoxide dismutase from teleost fish skin
19. Subunit interaction enhances enzyme activity and stability of sweet potato cytosolic Cu/Zn-superoxide dismutase purified by a His-tagged recombinant protein method
20. Effect of cadmium on antioxidant enzyme activities and lipid peroxidation in a freshwater field crab, Barytelphusa guerini
21. The paradigm that all oxygen-respiring eukaryotes have cytosolic Cu/Zn-superoxide dismutase and that Mn-superoxide dismutase is localized to the mitochondria does not apply to a large group of marine arthropods
22. Effect of trivalent and hexavalent chromium on antioxidant enzyme activities and lipid peroxidation in a freshwater field crab, Barytelphusa guerini