AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli

Nature

Volumn 419, Issue 6903, 2002, Pages 178-182

  Falnes, Pål Ø ^a   Johansen, Rune F ^a   Seeberg, Erling ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; DNA; ENZYMES; PROTEINS;

REPLICATION BLOCKS;

ESCHERICHIA COLI;

2 OXOGLUTARIC ACID; BACTERIAL DNA; DNA BINDING PROTEIN; PROTEIN ALKB; UNCLASSIFIED DRUG;

DNA;

ARTICLE; DEMETHYLATION; DNA REPAIR; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ADENINE; ALKYLATION; BASE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA DAMAGE; DNA METHYLATION; DNA REPAIR; DNA REPLICATION; DNA, BACTERIAL; DNA, SINGLE-STRANDED; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FORMALDEHYDE; MIXED FUNCTION OXYGENASES; OXIDATION-REDUCTION; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0037068433     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature01048     Document Type: Article

References (19)

1. The DNA-repair protein AlkB, EGL-9, and leprecan define new families of 2-oxoglutarate- and iron-dependent dioxygenases
2. Repair of O(6)-alkylguanine by alkyltransferases
3. Adaptation to alkylation resistance involves the induction of a DNA glycosylases
4. Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents
5. Adaptive response to alkylating agents involves alternation in situ of O6-methylguanine residues in DNA
6. A new gene (alkB) of Escherichia coli that controls sensitivity to methyl methane sulfonate
7. Molecular cloning and functional analysis of a human cDNA encoding an Escherichia coli AlkB homolog, a protein involved in DNA alkylation damage repair
8. Defective processing of methylated single-stranded DNA by E. coli AlkB mutants
9. The colorimetric estimation of formaldehyde by means of the Hantzsch reaction
10. A fluorimetric assay for hydrogen peroxide, suitable for NAD(P)H-dependent superoxide generating redox systems
11. The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity
12. Excision of 3-methylguanine from alkylated DNA by 3-methyladenine DNA glycosylase 1 of Escherichia coli
13. Replication of M 13 single-stranded viral DNA bearing single site-specific adducts by Escherichia coli cell extracts; differential efficiency of translesion DNA synthesis for SOS-dependent and SOS-independent lesions
14. Mechanisms of mutagenesis by exocyclic DNA adducts. Transfection of M 13 viral DNA bearing a site-specific adduct shows that ethenocytosine is a highly efficient RecA-independent mutagenic noninstructional lesion
15. One-step preparation of competent Escherichia coli: Transformation and storage of bacterial cells in the same solution
16. Reduction of oxidation during the preparation of DNA and analysis of 8- hydroxy-2κ-deoxyguanosine