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Volumn 124, Issue 49, 2002, Pages 14520-14521

A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN 4; COLLAGEN TYPE 1; HYDROXYAPATITE; OSTEOCALCIN; OSTEOPONTIN; PURINE DERIVATIVE; PURMORPHAMINE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR RUNX2; UNCLASSIFIED DRUG;

EID: 0037065356     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0283908     Document Type: Article
Times cited : (207)

References (19)
  • 4
    • 0002640027 scopus 로고    scopus 로고
    • Bilezikian J.P., Raisz L.G., Rodan G.A., Eds. Academic Press, San Diego
    • Aubin, J. E.; Liu, F. In Principles of Bone Biology; Bilezikian, J. P., Raisz, L. G., Rodan, G. A., Eds.: Academic Press: San Diego, 1996; pp 51-67.
    • (1996) Principles of Bone Biology , pp. 51-67
    • Aubin, J.E.1    Liu, F.2
  • 7
    • 0011949646 scopus 로고    scopus 로고
    • Promega Corporation Madison WI
    • Technical Bulletin No. 280; Promega Corporation: Madison. WI, 1999.
    • (1999) Technical Bulletin No 280
  • 12
    • 0011978039 scopus 로고    scopus 로고
    • note
    • Generally, 2500 cells were plated in each well of a 384-well plate with 100 μL of medium, and 500 nL of the 1 mM compound solutions were added. After incubating for 4 days, cells were lysed, and ALP activities were assayed (see Supporting Information for details).
  • 13
    • 0011948235 scopus 로고    scopus 로고
    • note
    • (13) The promoter region of Cbfa1/Runx2 gene (1.8 kb) was cloned and inserted directly upstream of the luciferase gene. Cells were transfected with reporter plamid and plated in 96-well plates. After incubating for 4 days, luciferase activity was assayed using Bright-Glo Luciferase reagent (see Supporting Information for details).
  • 16
    • 0011917664 scopus 로고    scopus 로고
    • note
    • Cells were fixed with 10% formalin solution and stained with an ALP staining kit from Sigma Diagnostics (see Supporting Information for details).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.