Molecular cloning of the heparin/heparan sulfate Δ4,5 unsaturated glycuronidase from Flavobacterium heparinum, its recombinant expression in Escherichia coli, and biochemical determination of its unique substrate specificity

Biochemistry

Volumn 41, Issue 23, 2002, Pages 7424-7434

  Myette, James R ^a   Shriver, Zachary ^a   Kiziltep e, Tanyel ^a   McLean, Maitland W ^b,c   Venkataraman, Ganesh ^a   Sasisekharan, Ram ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b UNIVERSITY OF ABERDEEN   (United Kingdom)

^c Nisthouse Harray   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

HEPARAN SULFATE;

CLONING; ENZYMES; ESCHERICHIA COLI; HYDROLYSIS;

BIOCHEMISTRY;

BETA GLUCURONIDASE; CHONDROITIN SULFATE; DERMATAN SULFATE; HEPARAN SULFATE; HEPARIN; HEPARIN LYASE; HYALURONIC ACID; OLIGOSACCHARIDE; URONIC ACID;

AMINO ACID SEQUENCE; ARTICLE; ENZYME DEGRADATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; FLAVOBACTERIUM; FLAVOBACTERIUM HEPARINUM; GENOME; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DISACCHARIDES; ESCHERICHIA COLI; FLAVOBACTERIUM; GENES, BACTERIAL; GENOME, BACTERIAL; GLUCURONIDASE; HEPARIN; HEPARITIN SULFATE; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FLAVOBACTERIUM; PEDOBACTER HEPARINUS;

EID: 0037062622     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi012147o     Document Type: Article

References (32)