Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane

Journal of Cell Biology

Volumn 159, Issue 5, 2002, Pages 845-854

  Bauer, Jörg ^c   Hiltbrunner, Andreas ^a,d   Weibel, Petra ^a,d   Vidi, Pierre Alexandre ^a,d   Alvarez Huerta, Mayte ^a,d   Smith, Matthew D ^b   Schnell, Danny J ^b   Kessler, Felix ^a,d  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c BASF SE   (Germany)

^d UNIVERSITY OF NEUCHÂTEL   (Switzerland)

Author keywords

Arabidopsis; Chloroplast biogenesis; GTP; Molecular switch; Receptor targeting

Indexed keywords

ATTOC159 PROTEIN; ATTOCC33 PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; PROTEIN PRECURSOR; RECEPTOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BIOGENESIS; CHLOROPLAST; CYTOSOL; NONHUMAN; NUCLEOTIDE SEQUENCE; OUTER MEMBRANE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BINDING SITES; CHLOROPLASTS; CYTOSOL; GREEN FLUORESCENT PROTEINS; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; GUANOSINE TRIPHOSPHATE; INTRACELLULAR MEMBRANES; LUMINESCENT PROTEINS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PLANTS, GENETICALLY MODIFIED; POINT MUTATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

ARABIDOPSIS;

EID: 0037049547     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200208018     Document Type: Article

References (36)

1. Bauer, J., K. Chen, A. Hiltbrunner, E. Wehrli, M. Eugster, D. Schnell, and F. Kessler. 2000. The major protein import receptor of plastids is essential for chloroplast biogenesis. Nature. 403:203-207.
2. Bauer, J., A. Hiltbrunner, and F. Kessler. 2001. Molecular biology of chloroplast biogenesis: Gene expression, protein import and intraorganellar sorting. Cell. Mol. Life Sci. 58:420-433.
3. Bechtold, N., and G. Pellerier. 1998. In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Methods Mol. Biol. 82:259-266.
4. Bölter, B., T. May, and J. Soll. 1998. A protein import receptor in pea chloroplasts, Toc86, is only a proteolytic fragment of a larger polypeptide. FEBS Lett. 441:59-62.
5. Chen, D., and D.J. Schnell. 1997. Insertion of the 34-kDa chloroplast protein import component, IAP34, into the chloroplast outer membrane is dependent on its intrinsic GTP-binding capacity. J. Biol. Chem. 272:6614-6620.
6. Chen, K., X. Chen, and D.J. Schnell. 2000a. Initial binding of preproteins involving the Toc159 receptor can be bypassed during protein import into chloroplasts. Plant Physiol. 122:813-822.
7. Chen, K., X. Chen, and D.J. Schnell. 2000b. Mechanism of protein import across the chloroplast envelope. Biochem. Soc. Trans. 28:485-491.
8. Cline, K. 2000. Gateway to the chloroplast. Nature. 403:148-149.
9. Connolly, T., and R. Gilmore. 1993. GTP hydrolysis by complexes of the signal recognition particle and the signal recognition particles receptor. J. Cell Biol. 123:799-807.
10. Fitzpatrick, L.M., and K. Keegstra. 2001. A method for isolating a high yield of Arabidopsis chloroplasts capable of efficient import of precursor proteins. Plant J. 27:59-65.
11. Gubler, U., and B.J. Hoffmann. 1983. A simple and very efficient method for generating cDNA libraries. Gene. 25:263-269.
12. Hiltbrunner, A., J. Bauer, M. Alvarez-Huerta, and F. Kessler. 2001 a. Protein translocon at the Arabidopsis outer chloroplast membrane. Biochem. Cell Biol. 79:1-7.
13. Hiltbrunner, A., J. Bauer, P.-A. Vidi, S. Infanger, P. Weibel, M. Hohwy, and F. Kessler. 2001. Targeting of an abundant cytosolic form of the protein import receptor atToc159 to the outer chloroplast membrane. J. Cell Biol. 154: 309-316.
14. Hinnah, S.C., K. Hill, R. Wagner, T. Schlicher, and J. Soll. 1997. Reconstitution of a chloroplast protein import channel. EMBO J. 16:7351-7360.
15. Hirsch, S., E. Muckel, F. Heemeyer, G. von Heijne, and J. Soll. 1994. A receptor component of the chloroplast protein translocation machinery. Science. 266: 1989-1992.
16. Jackson-Constan, D., and K. Keegstra. 2001. Arabidopsis genes encoding components of the chloroplastic protein import apparatus. Plant Physiol 125: 1567-1576.
17. Jarvis, P., L.J. Chen, H. Li, C.A. Peto, C. Fankhauser, and J. Chory. 1998. An Arabidopsis mutant defective in the plastid general protein import apparatus. Science. 282:100-103.
18. Jin, J.B., Y.A. Kim, S.J. Kim, S.H. Lee, D.H. Kim, G.W. Cheong, and I. Hwang. 2001. A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis. Plant Cell. 13: 1511-1526.
19. Keegstra, K., and K. Cline. 1999. Protein import and routing systems of chloroplasts. Plant Cell. 11:557-570.
20. Kessler, F., and D.J. Schnell. 2002. A GTPase gate for protein import into chloroplasts. Nat. Struct. Biol. 9:81-83.
21. Kessler, F., G. Blobel, H.A. Patel, and D.J. Schnell. 1994. Identification of two GTP-binding proteins in the chloroplast protein import machinery. Science. 266:1035-1039.
22. Liang, Z., T. Mather, and G. Li. 2000. GTPase mechanism and function: New insights from systematic mutational analysis of the phosphate-binding loop residue Ala30 of Rab5. Biochem. J. 346:501-508.
23. Ma, Y., A. Kouranov, S.E. LaSala, and D.J. Schnell. 1996. Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope. J. Cell Biol. 134:315-327.
24. Muckel, E., and J. Soll. 1996. A protein import receptor of chloroplasts is inserted into the outer envelope membrane by a novel pathway. J. Biol. Chem. 271: 23846-23852.
25. Perry, S.E., and K. Keegstra. 1994. Envelope membrane proteins that interact with chloroplastic precursor proteins. Plant Cell. 6:93-105.
26. Rensink, W.A., M. Pilon, and P. Weisbeek. 1998. Domains of a transit sequence required for in vivo import in Arabidopsis chloroplasts. Plant Physiol. 118: 691-699.
27. Schleiff, E., and J. Soll. 2000. Travelling of proteins through membranes: Translocation into chloroplasts. Planta. 211:449-456.
28. Schleiff, E., and R.B. Klösgen. 2001. Without a little help from 'my' friends: Direct insertion of proteins into chloroplast membranes? Biochim. Biophys. Acta. 1541:22-33.
29. Schleiff, E., J. Soll, N. Sveshnikova, R. Tien, S. Wright, C. Dabney-Smith, C. Subramanian, and B.D. Bruce. 2002. Structural and guanosine triphosphate/diphosphate requirements for transit peptide recognition by the cytosolic domain of the chloroplast outer envelope receptor, Toc34. Biochemistry. 41: 1934-1946.
30. Schnell, D.J., F. Kessler, and G. Blobel. 1994. Isolation of components of the chloroplast protein import machinery. Science. 266:1007-1012.
31. Smith, M., A. Hiltbrunner, F. Kessler, and D.J. Schnell. 2002. The role of GTP in the targeting and insertion of atToc159, the major preprotein receptor of chloroplasts. J. Cell Biol. 159:833-843.
32. Sun, Y.J., F. Forouhar, H. Li, S. Tu, Y.H. Yeh, S. Kao, H.L. Shr, C.C. Chou, C. Chen, and C.D. Hsiao. 2002. Crystal structure of pea Toc34, a novel GTPase of the chloroplast protein translocon. Nat. Struct. Biol. 9:95-100.
33. Sveshnikova, N., J. Soll, and E. Schleiff. 2000. Toc34 is a preprotein receptor regulated by GTP and phosphorylation. Proc. Natl. Acad. Sci. USA. 97:4973-4978.
34. Tinland, B., F. Schoumacher, V. Gloeckler, A.M. Bravo-Angel, and B. Hohn. 1995. The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome. EMBO J. 14:3585-3595.
35. Tranel, P.J., and K. Keegstra. 1996. A novel, bipartite transit peptide targets OEP75 to the outer membrane of the chloroplastic envelope. Plant Cell. 8:2093-2104.
36. Wessel, D., and U.-I. Flügge. 1984. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal. Biochem. 138:141-143.