Analogues of aspartic proteases synthesized by densely covering silica gel with carboxyl groups

Bioorganic and Medicinal Chemistry Letters

Volumn 12, Issue 19, 2002, Pages 2663-2666

  Kim, Hyunsook ^a   Chung, Yeon Sook ^a   Paik, Hyesun ^a   Kim, Myoung Soon ^a   Suh, Junghun ^a  

^a Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEINASE; CARBOXYL GROUP; HEMOGLOBIN; IMMUNOGLOBULIN; SILICA GEL;

ADSORPTION; ARTICLE; CATALYSIS; COMPLEX FORMATION; ENZYME ACTIVE SITE; ENZYME SYNTHESIS; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS;

ADSORPTION; ALBUMINS; ASPARTIC ENDOPEPTIDASES; CATALYSIS; GAMMA-GLOBULINS; HEMOGLOBINS; HYDROLYSIS; KINETICS; OVALBUMIN; PEPTIDES; SILICON DIOXIDE;

EID: 0037037343     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(02)00567-X     Document Type: Article

References (17)

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13. At pH 4-7, a significant portion of carboxyl groups of Si(A)-Suc and Si(A)-Suc-Ac would be present as carboxylate anions, and amino groups of the proteins are to be mostly protonated. Thus, protein adsorption would be significant at weakly acid pHs, although the degree of adsorption depends on the nature of proteins.
14. 9,10 This indicates that the rate data mostly represent the protein degradation.
15. The adsorption of the proteins to Si(A)-Suc or Si(A)-Suc-Ac is not correlated with pI (isoelectric point) values (4.5 for ovalbumin, 4.7 for albumin, 6.8 for hemoglobin, and 7.2 for γ-globulin).
16. As mentioned above, some amino groups of Si(A) are inaccessible to succinic anhydride or acetic anhydride most likely due to steric strain. Interaction of such amino groups of silica gel with bulky molecules such as protein substrates or hydrolysates would be insignificant.
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