Quantitative RT-PCR analysis demonstrates that synthesis of the recombinant fibrinogen is dependent on the transcription and synthesis of γ-chain

Clinica Chimica Acta

Volumn 319, Issue 1, 2002, Pages 67-73

  Hirota Kawadobora, Masako ^a,b   Tozuka, Minoru ^a   Yamauchi, Kazuyoshi ^a   Hidaka, Eiko ^a   Ueno, Ichiro ^a   Sugano, Mitsutoshi ^a   Terasawa, Fumiko ^c   Okumura, Nobuo ^c   Katsuyama, Tsutomu ^b   Shigematsu, Hidekazu ^b  

^a SHINSHU UNIVERSITY HOSPITAL   (Japan)

^b SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c SHINSHU UNIVERSITY   (Japan)

Author keywords

Chain; Fibrinogen synthesis; mRNA; Quantitative RT PCR; Recombinant fibrinogen

Indexed keywords

FIBRINOGEN; MESSENGER RNA; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL LYSATE; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORRELATION FUNCTION; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; IN VIVO STUDY; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; CELL EXTRACTS; CHO CELLS; CRICETINAE; CULTURE MEDIA, CONDITIONED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBRINOGEN; HUMANS; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

CRICETINAE; CRICETULUS GRISEUS; VECTORS;

EID: 0037035495     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(02)00022-0     Document Type: Article

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