Recombinant expression and in vitro folding of proinsulin are stimulated by the synthetic dithiol Vectrase-P

FEMS Microbiology Letters

Volumn 213, Issue 2, 2002, Pages 225-230

  Winter, Jeannette ^a   Lilie, Hauke ^a   Rudolph, Rainer ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Disulfide bond formation; Oxidative folding; Periplasm; Proinsulin; Secretory expression

Indexed keywords

DITHIOL DERIVATIVE; PROINSULIN; PROTEIN DISULFIDE ISOMERASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VECTRASE P; 1,2 BIS(2 MERCAPTOACETAMIDO)CYCLOHEXANE; 1,2-BIS(2-MERCAPTOACETAMIDO)CYCLOHEXANE; CYCLOHEXANE DERIVATIVE; DRUG DERIVATIVE; HYBRID PROTEIN; THIOL DERIVATIVE; TOLUENE;

ARTICLE; BACTERIUM CULTURE; CELL COMPARTMENTALIZATION; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC RECOMBINATION; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN SYNTHESIS; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; IN VITRO STUDY; SYNTHESIS;

CYCLOHEXANES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; IN VITRO; PROINSULIN; PROTEIN DISULFIDE-ISOMERASE; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; SULFHYDRYL COMPOUNDS; SUPPORT, NON-U.S. GOV'T; TOLUENE;

ESCHERICHIA COLI;

EID: 0037031138     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)00819-4     Document Type: Article

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