Controlled enzymatic removal of damaging casein layers on medieval wall paintings

Biotechnology and Bioengineering

Volumn 80, Issue 1, 2002, Pages 13-21

  Beutel, Sascha ^a   Klein, Kerstin ^b   Knobbe, Gabi ^c   Königfeld, Peter ^b   Petersen, Karin ^c   Ulber, Roland ^a   Scheper, Thomas ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b Niedersächsisches Landesamt für Denkmalpflege ^*  (Germany)

^c UNIVERSITY OF OLDENBURG   (Germany)

Author keywords

2D fluorescence analysis; Alcalase 2.5 DX L; Casein; Medieval wall painting; Noninvasive surface analysis; Proteolysis; RPHPLC; Surface layer; Tryptophan

Indexed keywords

CASEIN; ENVIRONMENTAL IMPACT; ENZYMES; OPTICAL FIBERS; PEELING;

PROTEOLYSIS;

BIOTECHNOLOGY;

BUFFER; CARBONIC ACID; CASEIN; ENZYME; SUBTILISIN; TRYPTOPHAN;

ARTICLE; CHEMICAL REACTION; DESTRUCTION; ENVIRONMENTAL FACTOR; ENZYME ACTIVITY; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; METHODOLOGY; PAINTING; PROTEIN BINDING; PROTEIN DEGRADATION;

CASEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYMES, IMMOBILIZED; HISTORY, MEDIEVAL; HYDROLYSIS; MEMBRANES, ARTIFICIAL; PAINTINGS; PEPTIDE HYDROLASES; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; SUBTILISINS;

EID: 0037026930     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10341     Document Type: Article

References (15)

1. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248.
2. Fritz E. 1992. Reinigungs- und Umwandlungsverfahren mit Ammoniumcarbonat-Möglichkeiten und Gefahren beim Einsatz an Wandmalereien. Thesis Fachhochschule Hildesheim.
3. Hillmer JM. 1996. In situ-Bioprozesskontrolle am Beispiel der in vivo-Biolumineszenz und der 2D-Fluoreszenzspektroskopie. PhD thesis, Universität Hannover.
4. Klein AE, Freiberg J, Same S, Carrol MA. 1989. Rapid colorimetric determination of activity of subtilisin enzymes in cleaning products. J Assoc Anal Chem 72:881.
5. Matsumoto I, Mizuno Y, Seno N. 1979. Activation of sepharose with epichlorhydrin and subsequent immobilization of ligand for affinity absorbent. J Biochem (Tokyo), 85:1091.
6. Möller H-H. 1990. Schäden an Wandmalereien und ihre Ursachen, Arbeitshefte zur Denkmalpflege in Niedersachsen 8. Hameln.
7. Nishio T, Hayashi R. 1984. Digestion of protein substrates by subtilisin: immobilization changes the pattern of products. Arch Biochem Biophys 229:304.
8. Ostoa-Saloma P, Ramirez J, Perez-Montfort R. 1989. Measurement of casein digestion by a fluorimetric method. Anal Biochem 176:239.
9. Ostoa-Saloma P, Ramirez J, Perez-Montfort R. 1990. Causes of the decrease in fluorescence due to proteolysis of a-casein. Biochim Biophys Acta 1041:146.
10. Paterson M, Kennedy JF. 1997. Cellulose paper support for immobilization. In: Bickerstaff GF, editor. Methods in biotechnology, vol. 1. Immobilization of enzymes and cells. Totowa, NJ: Humana Press. p 155.
11. Pfund H. 1991. Überlegungen und Versuche zum Einsatz von Enzymen in der Wandmalereirestaurierung. Thesis Fachhochschule Köln.
12. Preuss G. 1991. Enzymatische Entfernung von Caseinüberzügen. Seminar work Hochschule für bildende Künste Dresden.
13. Raschle P. 1985. Historische Technologie und Konservierung von Wandmalerei. Lecture texts of the 3 further educational seminar of the class of conservation and restoration. Bern: Schule für Gestaltung Bern.
14. Sedmak JJ, Grossberg SE. 1977. A rapid, sensitive and versatile assay for protein using Coomassie Brilliant Blue G 250. Anal Biochem 79:544.
15. Vanderkooi JM. 1992. Topics in fluorescence spectroscopy-tryptophan fluorescence from proteins at room temperature. J. R. Lakowicz editor. Plenum Press, New York. 3, pp 113.