Enzymatic hydrolysis of nitriles by an engineered nitrile hydratase (papain Gln19Glu) in aqueous-organic media

Biotechnology and Bioengineering

Volumn 79, Issue 1, 2002, Pages 9-14

  Versari, Andrea ^a,b   Ménard, Robert ^a   Lortie, Robert ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Amidase; Bioconversion; Nitrilase; Nitrile hydratase; Papain

Indexed keywords

ACETONE; ENZYMES; HYDROLYSIS; SOLUTIONS; SUBSTRATES;

ENZYMATIC HYDROLYSIS;

NITROGEN COMPOUNDS;

ACETONE; AMIDASE; CYSTEINE PROTEINASE; NITRILASE; NITRILE; NITRILE HYDRATASE; ORGANIC COMPOUND; PAPAIN;

AQUEOUS SOLUTION; ARTICLE; CHEMICAL STRUCTURE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SUBSTRATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MICHAELIS CONSTANT; MUTANT; PICHIA PASTORIS; REACTION TIME;

HYDRO-LYASES; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; NITRILES; ORGANIC CHEMICALS; PAPAIN; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SOLUTIONS; WATER;

PICHIA; PICHIA PASTORIS;

EID: 0037025152     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10267     Document Type: Article

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