A GFP-based screen for growth-arrested, recombinant protein-producing cells

Biotechnology and Bioengineering

Volumn 79, Issue 1, 2002, Pages 74-82

  Yuk, Inn H Y ^a   Wildt, Stefan ^a   Jolicoeur, Mario ^b   Wang, Daniel I C ^a   Stephanopoulos, Gregory ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

Author keywords

Bicistronic retroviral vectors; CHO cells; GFP based screen; Growth arrest; Recombinant protein expression

Indexed keywords

CELLS; FLUORESCENCE; GENES; GROWTH KINETICS; VIRUSES;

SERUM DEPRIVATION;

PROTEINS;

GAMMA INTERFERON; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN; RETROVIRUS VECTOR;

ANCHORAGE DEPENDENT GROWTH; ANIMAL CELL; ARTICLE; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION SYSTEM; GENETIC TRANSFECTION; HELPER CELL; NONHUMAN; PROTEIN ENGINEERING;

ANIMALS; BIOLOGICAL ASSAY; CELL SEPARATION; CHO CELLS; CRICETINAE; CRICETULUS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 0037025151     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10293     Document Type: Article

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