Bioaffinity purification of NADP+-dependent dehydrogenases: Studies with alcohol dehydrogenase from Thermoanaerobacter brockii

Biotechnology and Bioengineering

Volumn 77, Issue 5, 2002, Pages 517-527

  McMahon, Mary ^a   Mulcahy, Patricia ^a  

^a Ireland   (Ireland)

Author keywords

8 azo linked immobilized NADP+; Alcohol dehydrogenase; C8 linked immobilized NADP+; Kinetic locking on tactic; N6 linked immobilized NADP+; Stripping ligand tactic; Thermoanaerobacter brockii

Indexed keywords

ADSORPTION; AFFINITY CHROMATOGRAPHY; DERIVATIVES; ELECTROPHORESIS; PURIFICATION; SUBSTRATES;

LIGANDS;

ENZYMES;

ALCOHOL DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE;

AFFINITY CHROMATOGRAPHY; ANAEROBIC BACTERIUM; ARTICLE; ENZYME ASSAY; ENZYME KINETICS; ENZYME PURIFICATION; NONHUMAN; SYNTHESIS; THERMOANAEROBACTER BROCKII;

ALCOHOL DEHYDROGENASE; AZO COMPOUNDS; BACTERIA, ANAEROBIC; BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; ENZYMES, IMMOBILIZED; LIGANDS; NADP; PROTEIN BINDING;

THERMOANAEROBACTER; THERMOANAEROBACTER BROCKII;

EID: 0037022982     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10079     Document Type: Article

References (36)

1. +-preferred secondary alcohol dehydrogenase from Thermoanaerobium brockii
2. Media formulations
3. Thermoanaerobacter brockii alcohol dehydrogenase: Characterization of the active site metal and its ligand amino acids
4. A rapid and sensitive method for quantitation of microgram amounts of protein utilising the principal of protein-dye binding
5. The enzymes from extreme thermophiles: Bacterial sources, thermostabilities and industrial relevance
6. Potential applications of NAD(P)-dependent oxidoreductases in synthesis: A survey
7. Dehydrogenases for the synthesis of chiral compounds
8. DNS colorimetric determination of available carbohydrates
9. Synthetic applications of alcohol dehydrogenase from Thermoanaerobium brockii
10. Enzyme engineering aspects of biocatalysts: Cofactor regeneration as example
11. Cleavage of structural proteins during the assembly of bacteriophage T4
12. Novel NADP-linked alcohol/ketone oxidoreductase in thermophilic ethanologenic bacteria
13. Potential applications of an alcohol-aldehyde/ketone oxidoreductase from thermophilic bacteria
14. Characteristics of 8-substituted adenine nucleotide derivatives utilized in affinity chromatography
15. + analogue: Its application in affinity chromatography and as a functioning coenzyme
16. + and their potential as active coenzymes and affinity adsorbents
17. + as general ligands
18. +-derivatives: Studies with mammalian and microbial glutamate dehydrogenases
19. Both D- and L-lactate specific dehydrogenases coexist in individual cephalopods
20. Biospecific affinity purification of octopine dehydrogenase from molluscs
21. +-dependent dehydrogenases using the kinetic locking-on strategy
22. Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus lichenformis TSN9
23. +-dependent dehydrogenases; Studies with bovine L-lactate dehydrogenase
24. +-dependent dehydrogenases using the locking-on tactic
25. The kinetic locking-on strategy for bioaffinity purification; further studies with bovine liver glutamate dehydrogenase
26. A kinetic locking-on strategy for bioaffinity purification; further studies on alcohol dehydrogenase
27. +-dependent dehydrogenases
28. Biochemistry and biotechnology of amino acid dehydrogenases
29. Distribution, purification and characterization of thermostable phenylalanine dehydrogenase from thermophilic Actinomycetes
30. Steady-state kinetic mechanism of Thermoanaerobium brockii alcohol dehydrogenase: A study of discrimination between alternative models
31. Activities of phosphomonoesterase and phosphodiesterase from Lumbricus terrestris
32. Kinetic mechanism of the oxidation of 2-propanol catalyzed by Thermoanaerobium brockii alcohol dehydrogenase
33. Amino acid sequence of alcohol dehydrogenase from the thermophilic bacterium Thermoanaerobium brockii
34. Application of immobilized cells of Thermoanaerobium brockii for stereoselective reductions of exoacid esters
35. +-dependent dehydrogenases
36. The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures