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Volumn 12, Issue 21, 2002, Pages 3093-3096

A general synthesis of specifically deuterated nucleotides for studies of DNA and RNA

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOTIDE; DEUTERIUM; DNA; NUCLEIC ACID; NUCLEOSIDE; RIBONUCLEOTIDE; RIBONUCLEOTIDE REDUCTASE; RNA;

EID: 0037020717     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(02)00650-9     Document Type: Article
Times cited : (17)

References (18)
  • 13
    • 84992660014 scopus 로고    scopus 로고
    • note
    • 3-Phosphoglycerate mutase was purchased from Boehringer Mannheim. Enolase, myokinase, pyruvate kinase, and guanylate kinase were purchased from Sigma. We obtained as the generous gifts of Jamie Williamson bacterial expression strains for ribokinase, PRPP synthetase, adenine phosphoribosyltransferase, and xanthine-guanine phosphoribosyltransferase. We expressed and purified these enzymes using the procedures described in ref 7.
  • 15
    • 84992660011 scopus 로고    scopus 로고
    • note
    • 3); deuterium content 99%.
  • 16
    • 84992565711 scopus 로고    scopus 로고
    • note
    • We obtained the ribonucleotide reductase expression plasmid pSquire as a generous gift from JoAnne Stubbe. We expressed and purified the enzyme as described in ref 17.
  • 18
    • 84992565706 scopus 로고    scopus 로고
    • note
    • 2H]ribose.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.