Prolonged shearing of insect cells in a couette bioreactor

Enzyme and Microbial Technology

Volumn 31, Issue 5, 2002, Pages 600-608

  O'Connor, Kim C ^a,b   Cowger, Nancy L ^a,b   De Kee, Daniel C R ^a   Schwarz, Ray P ^c  

^a TULANE UNIVERSITY   (United States)

^b TULANE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Synthecon Corporation   (United States)

Author keywords

Apoptosis; Couette bioreactor; Insect cells; Necrosis; Shear; Viscosity

Indexed keywords

BIOREACTORS; CELL CULTURE; NEWTONIAN LIQUIDS; PLASTICITY; POPULATION STATISTICS; VISCOMETERS;

LOW SHEAR LEVELS;

CELLS;

ANIMAL CELL; APOPTOSIS; ARTICLE; BIOREACTOR; CELL DEATH; CELL DENSITY; CELL GROWTH; CELL POPULATION; CELL VIABILITY; COMPARATIVE STUDY; CONTROLLED STUDY; HALF LIFE TIME; HYDRODYNAMICS; INSECT CELL CULTURE; LEPIDOPTERA; LIQUID; METHODOLOGY; NONHUMAN; PREDICTION; SHEAR STRESS; VISCOMETER; VISCOMETRY; VISCOSITY;

INSECTA; SPODOPTERA; SPODOPTERA FRUGIPERDA;

EID: 0037015406     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00137-0     Document Type: Article

References (37)

1. Chen K., Liu Q., Xie L., Sharp P.A., Wang D.I.C. Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production. Biotechnol. Bioeng. 72:2001;55-61.
2. Farrell P.J., Maolong L., Prevost J., Brown C., Behie L., Iatrou K. High-level expression of secreted glycoproteins in transformed lepidopteran insect cells using a novel expression vector. Biotechnol. Bioeng. 60:1998;656-663.
3. Papoutsakis E.T. Fluid-mechanical damage of animal cells in bioreactors. Trends Biotechnol. 9:1991;427-437.
4. Mclntire L.V., Wagner J.E., Papadaki M., Whitson P.A., Eskin S.E. Effect of flow on gene regulation in smooth muscle cells and macromolecular transport across endothelial cell monolayers. Biol. Bull. 194:1998;394-399.
5. Goldblum S., Bae Y.-K., Hink W.F., Chalmers J. Protective effect of methylcellulose and other polymers on insect cells subjected to laminar shear stress. Biotechnol. Prog. 6:1990;383-390.
6. Palomares L.A., Gonzalez M., Ramirez O.T. Evidence of pluronic F-68 direct interaction with insect cells: impact on shear protection, recombinant protein, and baculovirus production. Enzyme Microb. Technol. 26:2000;324-331.
7. Motobu M., Wang P.C., Matsumura M. Effect of shear stress on recombinant Chinese hamster ovary cells. J. Ferment. Bioeng. 85:1998;190-195.
8. Ramírez O.T., Mutharasan R. The role of the plasma membrane fluidity on the shear sensitivity of hybridomas grown under hydrodynamic stress. Biotechnol. Bioeng. 36:1990;911-920.
9. Cowger N.L., O'Connor K.C., Hammond T.G., Lacks D.J., Navar G.L. Characterization of bimodal cell death of insect cells in a rotating-wall vessel and shaker flask. Biotechnol. Bioeng. 64:1999;14-26.
10. Dunlop E.H., Namdev P.K., Rosenberg M.Z. Effect of fluid shear forces on plant cell suspensions. Chem. Eng. Sci. 49:1994;2263-2276.
11. Leverett L.B., Hellums D., Alfrey C.P., Lynch E.C. Red blood cell damage by shear stress. Biophys. J. 12:1972;257-273.
12. Abu-Reesh I., Kargi F. Biological responses of hybridoma cells to defined hydrodynamic shear stress. J. Biotechnol. 9:1989;167-178.
13. Couette M. Étude sur le frottement des liquides. Ph.D. thesis, Faculté des Sciences de Paris, 1890.
14. Andereck C.D., Liu S.S., Swinney H.L. Flow regimes in a circular Couette system with independently rotating cylinders. J. Fluid. Mech. 164:1986;155-183.
15. Thomas N.H., Janes D.A. Fluid dynamic considerations in airlift and annular vortex bioreactors for plant cell culture. Ann. NY Acad. Sci. 506:1987;171-189.
16. Tsao Y.-M.D., Boyd E., Wolf D.A., Spaulding G. Fluid dynamics within a rotating bioreactor in space and earth environments. J. Spacecr. Rockets. 31:1994;937-943.
17. Van Wazer JR, Lyons JW, Kim KY, Colwell RE. Viscosity and flow measurements: a laboratory handbook of rheology. New York: Wiley, 1963.
18. Meneses-Acosta A., Mendonça R.Z., Merchant H., Covarrubias L., Ramirez O.T. Comparative characterization of cell death between Sf9 insect cells and hybridoma cultures. Biotechnol. Bioeng. 72:2001;441-457.
19. Carreau PJ, De Kee DCR, Chhabra RP. Rheology of polymeric systems: principles and applications. Munich: Hanser, 1997.
20. Landau LD, Lifshitz EM. Fluid mechanics. 2nd ed. Oxford: Pergamon Press, 1987.
21. Calderbank P.H., Moo-Young M.B. The prediction of power consumption in the agitation of non-Newtonian fluids. Trans. Instn. Chem. Eng. 37:1959;26-33.
22. Krieger I.M., Maron S.H. Direct determination of the flow curves of non-Newtonian fluids. III. standardized treatment of viscometric data. J. Appl. Phys. 25:1954;72-75.
23. Simpson N.H., Milner A.E., Al-Rubeai M. Prevention of hybridoma cell death by bcl-2 during suboptimal culture conditions. Biotechnol. Bioeng. 54:1997;1-16.
24. Adams J.M., Cory S. Life-or-death decisions by the Bcl-2 protein family. Trends Biochem. Sci. 26:2001;61-66.
25. Voet D, Voet JG, Pratt CW. Fundamentals of biochemistry. New York: Wiley, 1999.
26. Thurston G.B. Viscoelasticity of human blood. Biophys. J. 12:1972;1205-1217.
27. Ballica R., Ryu D.D.Y., Powell R.L., Owen D. Rheological properties of plant cell suspensions. Biotechnol. Prog. 8:1992;413-420.
28. Olsvik E., Tucker K.G., Thomas C.R., Kristiansen B. Correlation of Aspergillus niger broth rheological properties with biomass concentration and the shape of mycelial aggregates. Biotechnol. Bioeng. 42:1993;1046-1052.
29. Shi Y., Ryu D.D.Y., Ballica R. Rheological properties of mammalian cell culture suspensions: hybridoma and HeLa cell lines. Biotechnol. Bioeng. 41:1993;745-754.
30. Brady J.F., Bossis G. The rheology of concentrated suspensions of spheres in simple shear flow by numerical simulation. J. Fluid Mech. 155:1985;105-129.
31. Aoyagi T., Doi M. Molecular dynamics simulation of entangled polymers in shear flow. Comput. Theor. Polym. S. 10:2000;317-321.
32. Curtis W.R., Emery A.H. Plant cell suspension culture rheology. Biotechnol. Bioeng. 42:1993;520-526.
33. Rodríguez-Monroy M., Galindo E. Broth rheology, growth and metabolite production of Beta vulgaris suspension culture: a comparative study between cultures grown in shaker flasks and in a stirred tank. Enzyme Microb. Technol. 24:1999;687-693.
34. Leighton D., Acrivos A. The shear-induced migration of particles in concentrated suspensions. J. Fluid Mech. 181:1987;415-439.
35. Dealy J. Rheometers for molten plastics. New York: Van Nostrand and Reinhold, 1982.
36. Freed L.E., Vurjak-Novakovic G. Cultivation of cell-polymer tissue constructs in simulated microgravity. 46:1995;306-313.
37. Cowger N.L., O'Connor K.C., Bivins J.F. Influence of simulated microgravity on the longevity of insect-cell culture. Enzyme Microb. Technol. 20:1997;326-332.