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0345894083
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note
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The cytoplasmic membrane instability upon adsorption of strongly charged polyelectrolyte molecules has been overcome by pre-treating the cells with a cross-linking agent such as glutardialdehyde. The cells were incubated in 2% v/v glutaraldehyde for 60 min prior to the coating process.
-
-
-
-
30
-
-
0346524888
-
-
note
-
-1) was incubated for 10 min. The resulting solution was washed with NaCl (3 x 145 mmol) prior to the next adsorption step.
-
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31
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17044455766
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0347155317
-
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note
-
The coated cells were treated with a 1.2% w/v NaOCl solution for 20 min to remove the cytoplasmic constituents; the cells were then washed with NaCl (3 x 145 mmol).
-
-
-
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36
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0347155319
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unpublished video miscroscopy observations
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E. Donath, unpublished video miscroscopy observations.
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Donath, E.1
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0346524887
-
-
note
-
AFM measurements were conducted on a Nanoscope III in the tapping mode. A drop of the suspension containing the shells was allowed to dry on mica.
-
-
-
-
40
-
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0345894080
-
-
note
-
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0347155315
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note
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SEM measurements were conducted using a Zeiss DSM 40 instrument operated at an accelerating voltage of 15 KeV
-
-
-
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50
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0342902613
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