Molecular breeding of genes, pathways and genomes by DNA shuffling

Journal of Molecular Catalysis B: Enzymatic

Volumn 19-20, Issue , 2002, Pages 3-12

  Stemmer, Willem P C ^a  

^a MAXYGEN INC   (United States)

Author keywords

Breeding; DNA shuffling; Genomes

Indexed keywords

AMINO ACIDS; DNA; DNA SEQUENCES; MUTAGENESIS;

MOLECULAR BREEDING;

GENES;

ALPHA INTERFERON; ALPHA LEVO FUCOSIDASE; BETA LACTAMASE; CEPHALOSPORINASE; GREEN FLUORESCENT PROTEIN; SUBTILIN; THYMIDINE KINASE;

BREEDING; CLONE; CONFERENCE PAPER; DETOXIFICATION; DNA POLYMORPHISM; DNA SEQUENCE; DOG; GENE LIBRARY; GENETIC RECOMBINATION; GENETIC VARIABILITY; GENOME; MICROBIAL GENETICS; MOLECULAR CLONING; MOLECULAR EVOLUTION; MOLECULAR GENETICS; MUTAGENESIS; NONHUMAN; OPERON; PHENOTYPE;

EID: 0037010694     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1381-1177(02)00146-7     Document Type: Conference Paper

References (17)

1. Stemmer W.P.C. Searching sequence space. Biotechnology. 13:1995;549-553.
2. Stemmer W.P.C. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. U.S.A. 91:1994;10747-10751.
3. Stemmer W.P.C. Rapid evolution of a protein in vitro by DNA shuffling. Nature. 370:1994;389-391.
4. Crameri A., Whitehorn E., Tate E., Stemmer W.P.C. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14:1996;315-319.
5. Zhang J., Dawes G., Stemmer W.P.C. Evolution of an effective fucosidase from a galactosidase by DNA shuffling and screening. Proc. Natl. Acad. Sci. U.S.A. 94:1997;4504-4509.
6. Crameri A., Dawes G., Rodriguez E., Silver S., Stemmer W.P.C. Molecular Evolution of an arsenate detoxification pathway by DNA shuffling. Nat. Biotechnol. 15:1997;436-438.
7. Crameri A., Raillard S.-A., Bennudez E., Stemmer W.P.C. DNA shuffling of genes from diverse species accelerates directed evolution. Nature. 391:1998;288-291.
8. Ness J., Welch M., Giver L., Bueno M., Cherry J., Borchert T., Stemmer W.P.C., Minshull J. Creation of a functionally diverse enzyme library by DNA family shuffling. Nat. Biotechnol. 17:1999;893-896.
9. Christians F.C., Scapozza L., Crameri A., Folkers G., Stemmer W.P.C. Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling. Nat. Biotechnol. 17:1999;259-264.
10. Stemmer W.P.C., Crameri A., Ha K.D., Brennan T.M., Heyneker H.L. Single-step PCR assembly of a gene and a whole plasmid from large numbers of oligonucleotides. Gene. 164:1995;49-53.
11. Chang C.-C., Chen T.T., Cox B.W., Dawes G.N., Stemmer W.P.C., Punnonen J., Patten P.A. Rapid evolution of a cytokine using molecular breeding. Nat. Biotechnol. 17:1999;793-797.
12. Soong N.-W., Nomura L., Pekrun K., Reed M., Sheppard L., Dawes G., Stemmer W.P.C. Molecular breeding of viruses. Nat. Genet. 25:2000;436-439.
13. Powell S.K., Kaloss M.A., Pinkstaff A., McKee R., Burimski I., Pensiero M., Otto E., Stemmer W.P.C., Soong N.-W. Breeding of retroviruses by DNA shuffling for improved stability and processing yields. Nat. Biotechnol. 18:2000;1279-1282.
14. Stemmer W.P.C., Soong N.-W. Molecular breeding of viruses for targeting and other clinical properties. Tumor Targeting. 4:1999;59-62.
15. Powell K.A., Ramer S.W., del Cardayré S.B., Stemmer W.P.C., Tobin M.B., Longchamp P.F., Huisman G.W. Directed evolution and biocatalysis. Angew Chem. Int. Ed. 40:2001;3948-3959.
16. Patnaik R., Louie R., Perry K., Stemmer W.P.C., DelCardayré S.B. Genome shuffling of Lactobacillus: microbial breeding for tolerance to low pH. Nat. Biotechnol. 20:2002;707-712.
17. Zhang Y.-X., Perry K., Vinci V.A., Powell K., Stemmer W.P.C., DelCardayré S.B. Genome shuffling leads to rapid phenotypic improvement in bacteria. Nature. 15:2002;644-646.