Evaluation of an enzyme-linked immunosorbent assay for serological surveillance of infection with Actinobacillus pleuropneumoniae serotype 5 in pig herds

Veterinary Microbiology

Volumn 88, Issue 3, 2002, Pages 223-232

  Klausen, Joan ^a   Andresen, Lars Ole ^a   Barfod, Kristen ^b   Sørensen, Vibeke ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b Danske Slagterier   (Denmark)

Author keywords

Actinobacillus pleuropneumoniae; CFT; ELISA; Lipopolysaccharide; Pig bacteria; Pleuropneumonia; Serological assay

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL POLYSACCHARIDE; BACTERIUM ANTIBODY; BACTERIUM LIPOPOLYSACCHARIDE;

ACTINOBACILLUS PLEUROPNEUMONIAE; ANTIBODY DETECTION; ARTICLE; COMPLEMENT FIXATION TEST; CONTROLLED STUDY; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; INTERMETHOD COMPARISON; NONHUMAN; PNEUMONIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SENSITIVITY AND SPECIFICITY; SEROLOGY; SEROTYPE; SERUM; SWINE;

ACTINOBACILLUS INFECTIONS; ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; COMPLEMENT FIXATION TESTS; DENMARK; ENZYME-LINKED IMMUNOSORBENT ASSAY; PLEUROPNEUMONIA; SENSITIVITY AND SPECIFICITY; SPECIFIC PATHOGEN-FREE ORGANISMS; SWINE; SWINE DISEASES;

ACTINOBACILLUS; ACTINOBACILLUS PLEUROPNEUMONIAE; ACTINOBACILLUS PLEUROPNEUMONIAE SEROVAR 5; MYCOPLASMA; SUS SCROFA;

EID: 0037009263     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1135(02)00111-6     Document Type: Article

References (24)

1. Experimental vaccination of pigs with an Actinobacillus pleuropneumoniae serotype 5b capsular polysaccharide-tetanus toxoid conjugate
2. Detection of antibodies to Actinobacillus pleuropneumoniae serotype 12 in pig serum using a blocking enzyme-linked immunosorbent assay
3. Serodiagnosis of pleuropneumonia using enzyme-linked immunosorbent assay with capsular polysaccharide antigens of Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7
4. Development and evaluation of a mixed-antigen ELISA for serodiagnosis of Actinobacillus pleuropneumoniae serotypes 1, 5, and 7 infections in commercial swine herds
5. Standardized diagnostic complement-fixation method and adaptation to micro test
6. Colorimetric methods for determination of sugars and related substances
7. Evaluation of long chain lipopolysaccharides (LC-LPS) of Actinobacillus pleuropneumoniae serotype 5 for the serodiagnosis of swine pleuropneumonia
8. Comparison of virulence of different Actinobacillus pleuropneumoniae serotypes and biotypes using an aerosol infection model
9. Antigenic differences within Actinobacillus pleuropneumoniae serotype 1
10. Development of a blocking ELISA for serodiagnosis of Actinobacillus pleuropneumoniae: Potential use of monoclonal antibodies against Ap serotype 5b capsular polysaccharides and LPS
11. Evaluation of blocking ELISAs for serodiagnosis of Actinobacillus pleuropneumoniae serotype 5 infections based on monoclonal antibodies against capsular polysaccharides and LPS as well as swine-mouse heterohybridoma-derived monoclonal antibodies
12. Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum
13. An enzyme-linked immunosorbent assay, using an EDTA-extracted antigen for the serology of Haemophilus pleuropneumoniae
14. Blocking enzyme-linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serotype 2
15. Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 8
16. Serological characterization of Actinobacillus pleuropneumoniae biotype 1 strains antigenically related to both serotypes 2 and 7
17. Biochemical characterization of an antigenic saline extract of Actinobacillus pleuropneumoniae serotype 5 and identification of a serotype-specific antigen for ELISA serodiagnosis
18. Detection of an Actinobacillus pleuropneumoniae serotype 2 lipopolysaccharide (LPS) variant
19. Detection of antibodies against Actinobacillus pleuropneumoniae serotype 5 using an inhibition enzyme immunoassay
20. Evaluation of a monoclonal blocking ELISA and IHA for antibodies to Mycoplasma hyopneumoniae in SPF-pig herds
21. Optimization and standardization of an enzyme-linked immunosorbent assay protocol for serodiagnosis of Actinobacillus pleuropneumoniae serotype 5