Folding and stability of different oligomeric states of thiamin diphosphate dependent homomeric pyruvate decarboxylase

Biophysical Chemistry

Volumn 96, Issue 2-3, 2002, Pages 259-271

  Killenberg Jabs, Margrit ^a   Kern, Gunther ^b   Hübner, Gerhard ^c   Golbik, Ralph ^c  

^a Sepsis diagnostics expert group   (Germany)

^b ASTRAZENECA   (United Kingdom)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Association dissociation; Homomeric recombinant pyruvate decarboxylase; Kinetics; Oligomeric states; Protein folding; Thermodynamics

Indexed keywords

DIMER; MONOMER; OLIGOMER; PYRUVATE DECARBOXYLASE; TETRAMER; THIAMINE DERIVATIVE; UREA;

ARTICLE; DISSOCIATION; ENZYME ACTIVITY; FLOW KINETICS; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE; THERMODYNAMICS; YEAST; CHEMISTRY; CIRCULAR DICHROISM; DIMERIZATION; DRUG EFFECT; ENZYMOLOGY; KINETICS; METABOLISM; PROTEIN DENATURATION; STRUCTURE ACTIVITY RELATION; TEMPERATURE;

CIRCULAR DICHROISM; DIMERIZATION; KINETICS; PROTEIN DENATURATION; PROTEIN FOLDING; PYRUVATE DECARBOXYLASE; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE; UREA; YEASTS;

EID: 0037007450     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-4622(02)00017-0     Document Type: Article

References (32)

1. Untersuchungen über die Cocarboxylase
2. The amino group and steric factors in thiamin catalysis
3. Synchrotron radiation solution X-ray scattering study of the pH dependence of the quaternary structure of yeast pyruvate decarboxylase
4. The influence of the effectors of yeast pyruvate decarboxylase (PDC) on the conformation of the dimers and tetramers and their pH-dependent equilibrium
5. Hydroxyl-ion-induced subunit dissociation of yeast cytoplasmatic pyruvate decarboxylase
6. Molecular weight and coenzyme content of pyruvate decarboxylase from brewer's yeast
7. Crystal structure of the thiamin diphosphate-dependent enzyme pyruvate decarboxylase from the yeast Saccharomyces cerevisiae at 2.3 A resolution
8. Pyruvate decarboxylase III: Specificity restriction for thiamine pyrophosphate in the protein association step; Subunit structure
9. Preliminary crystallographic data for the thiamin diphosphate-dependent enzyme pyruvate decarboxylase from brewers' yeast
10. Catalytic centers in the thiamin diphosphate dependent enzyme pyruvate decarboxylase at 2.4-Å resolution
11. Thiamin diphosphate dependent enzymes: Transketolase, pyruvate oxidase and pyruvate decarboxylase
12. Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 Å resolution
13. A thiamin diphosphate binding fold revealed by comparison of the crystal structures of transketolase, pyruvate oxidase and pyruvate decarboxylase
14. Struktur und Wirkungsweise des aktiven Zentrums der Hefe-Pyruvatdecarboxylase
15. Ring stacking interactions between thiamin and planar molecules as seen in the crystal structure of a picronolate dihydrate complex
16. Active oligomeric states of pyruvate decarboxylase and their functional characterization
17. Biochemistry and physiology of thiamin diphosphate enzymes
18. Folding and association of proteins
19. Protein stability and protein folding
20. Protein folding
21. Role of Glu51 for cofactor binding and catalytic activity in pyruvate decarboxylase from yeast studied by site-directed mutagenesis
22. Isolierung der Hefecarboxylase und Untersuchung über die Aktivität des Enzyms in lebenden Zellen
23. (N-U) values in a thermodynamic cycle
24. Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl a-chymotrypsin using different denaturants
25. Engineered disulfide bonds as probes of the folding pathway of barnase: Increasing the stability of proteins against the rate of denaturation
26. Folding of barstar C40A/C82A/P27A and catalysis of the peptidyl-prolyl cis/trans isomerization by human cytosolic cyclophilin (Cyp18)
27. Folding of chymotrypsin inhibitor 2. 1. Evidence for a two-state transition
28. The use of fluorescence methods to monitor unfolding transitions in proteins
29. Effects of metal ions, thiamine diphosphate analogues and subunit interactions on the reconstitution behaviour of pyruvate decarboxylase from brewer's yeast
30. Thermodynamics of denaturation of staphylococcal nuclease mutants: An intermediate state in protein folding