Improved staining of negative binding sites with ruthenium red on cryosections of frozen cells

General Physiology and Biophysics

Volumn 21, Issue 4, 2002, Pages 457-461

  El Saggan, A H ^a   Uhrík, Branislav ^a  

^a INSTITUTE OF MOLECULAR PHYSIOLOGY AND GENETICS   (Slovakia)

Author keywords

Cell coat; Cryosections; Ruthenium red

Indexed keywords

CACODYLIC ACID; GLUTARALDEHYDE; GLYCOCONJUGATE; LIQUID NITROGEN; RUTHENIUM RED; SUCROSE;

ANIMAL CELL; BINDING SITE; CELL COMPARTMENTALIZATION; CELL CULTURE; CELL ISOLATION; CELL MEMBRANE; CENTRIFUGATION; CHEMICAL BINDING; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELECTRON MICROSCOPY; FROZEN SECTION; LEUKEMIA L 1210; MOUSE; NONHUMAN; REVIEW; STAINING;

ANIMALS; BINDING SITES; CRYOPRESERVATION; FROZEN SECTIONS; LEUKEMIA; MICE; RUTHENIUM RED; STAINING AND LABELING; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0037004664     PISSN: 02315882     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (11)

1. Drobná Z., Stein U., Walther W., Barančik M., Breier A. (2002): Pentoxifylline influences drug transport activity of P-glycoprotein and decreases mdr1 gene expression in multidrug resistant mouse leukemic L1210/VCR cells. Gen. Physiol. Biophys. 21, 103-109
2. Handley D. A., Chien S. (1981): Oxidation of ruthenium red for use as an intercellular tracer. Histochemistry 71, 249-258
3. Kvačkajová-Kišucká J., Barančik M., Breier A. (2001): Drug transporters and their role in multidrug resistance of neoplastic cells. Gen. Physiol. Biophys. 20, 215-237
4. Kišucká J., Barančik M., Boháčová V., Breier A. (2001): Reversal effect of specific inhibitors of extracellular-signal regulated protein kinase pathway on P-glycoprotein mediated vincristine resistance of L1210 cells. Gen. Physiol. Biophys. 20, 439-444
5. Luft J. H. (1964): Electron microscopy of cell extraneous coats as revealed by ruthenium red staining. J. Cell Biol. 23, 54A-55A
6. Luft J. H. (1971a): Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat. Rec. 171, 347-368
7. Luft J. H. (1971b): Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat. Rec. 171, 369-416
8. Rosenfeld C., Paintrand M., Choquet C., Venuat A. M. (1973): Cyclic variations in the ruthenium red stained coat of cells from a synchronized human lymphoblastoid line. Exo. Cell Res. 79, 465-468
9. Snowdowne K. W., Howell J. N. (1984): Ruthenium red: differential effects on excitation and excitation-contraction coupling in frog skeletal muscle. J. Muscle Res. Cell Motil. 5, 399-410
10. Vorbrodt A., Koprowski H. (1969): Ruthenium red-stained coat of normal and Simian virus 40-transformed cells. J. Nat. Cancer Inst. 43, 1241-1248
11. 2+ electrogenesis. Gen. Physiol. Biophys. 9, 545-568