Effects of hypoxia on retinal pigmented epithelium cells: Protection by antioxidants

Ophthalmic Research

Volumn 34, Issue 6, 2002, Pages 338-342

  Castillo, M ^b   Bellot, J L ^b   Garcia Cabanes C ^b   Miquel, J ^b   Orts, A ^b   Palmero, Mercedes ^a  

^a UNIVERSITY OF ALICANTE   (Spain)

^b NONE

Author keywords

Antioxidants; Free radicals; Hypoxia; N acetyl cysteine; RPE cells; Vitamin C

Indexed keywords

ACETYLCYSTEINE; ADENOSINE TRIPHOSPHATE; ANTIOXIDANT; ASCORBIC ACID; DNA FRAGMENT; FREE RADICAL; LACTATE DEHYDROGENASE;

ANIMAL CELL; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; CATTLE; CELL ACTIVITY; CELL AGING; CELL DAMAGE; CELL HYPOXIA; CELL PROTECTION; CONTROLLED STUDY; DNA DAMAGE; ENZYME ACTIVITY; EYE PROTECTION; INVOLUTION; NONHUMAN; PIGMENT EPITHELIUM; PRIORITY JOURNAL;

ACETYLCYSTEINE; ADENOSINE TRIPHOSPHATE; ANIMALS; ANTIOXIDANTS; ASCORBIC ACID; CATTLE; CELL DEATH; CELL HYPOXIA; CELL SURVIVAL; CELLS, CULTURED; DNA FRAGMENTATION; FREE RADICAL SCAVENGERS; OXYGEN; PIGMENT EPITHELIUM OF EYE;

EID: 0036974163     PISSN: 00303747     EISSN: None     Source Type: Journal    
DOI: 10.1159/000067050     Document Type: Article

References (22)

1. Steinberg RH, Wood I: The relationship of the retinal pigment epithelium; in Zinn KM, Marmor MF (eds): The Retinal Pigment Epithelium. Cambridge, Mass, Harvard University Press, 1979, pp 205-225.
2. Basinger SF, Hoffman RT: Biochemistry of the pigment epithelium; in Anderson RE (ed): Biochemistry of the Eye. San Francisco, American Academy of Ophthalmology Manuals Program, 1983, pp 256-264.
3. Augustin AJ, Hunt S, Breipohl W, Böker T, Spitznas M: Influence of oxygen free radicals and free radical scavengers on the growth behaviour and oxidative tissue damage of bovine retinal pigment epithelium cells in vitro. Graefe's Arch Exp Ophthalmol 1996;234:58-63.
4. Newsome DA, Dobart EP, Liles MR, Oliver PD: Human retinal epithelium contains two distinct species of superoxide dismutase. Invest Ophthalmol Vis Sci 1990;31:2508-2513.
5. Ueda Y, Armstrong D: Preventive effect of natural and synthetic antioxidants on lipid peroxidation in the mammalian eye. Ophthalmic Res 1996;28:184-192.
6. Hollis AL, Butcher WI, Davis H, Henderson RA, Stone WL: Structural alterations in retinal tissues from rats deficient in vitamin E and selenium and treated with hyperbaric oxygen. Exp Eye Res 1992;54:671-684.
7. Liles MR, Newsome DA, Oliver PD: Antioxidant enzymes in the aging human retinal pigment epithelium. Arch Ophthal 1991;109:1285-1288.
8. Becquet F, Goureau O, Soubrane G, Coscas G, Courtois Y, Hicks D: Superoxide inhibits proliferation and phagocytic internalization of photoreceptor outer segments by bovine retinal pigment epithelium in vitro. Exp Cell Res 1994;212:374-382.
9. Rego AC, Santos MS, Oliveira CR: Oxidative stress, hypoxia and ischemia-like conditions increase the release of endogenous amino acids by distinct mechanisms in cultured retinal cells. J Neurochem 1996;66:2506-2516.
10. Palmero M, Bellot J L, Castillo M, García-Cabanes C, Miquel J, Orts A: An in vitro model of ischemic-like stress in retinal pigmented epithelium cells: protective effects of antioxidants. Mech Ageing Dev 2000;113:185-190.
11. Miquel J: An update on the oxygen stress-mitochondrial mutation theory of aging: Genetic and evolutionary implications. Exp Gerontol 1998;33:113-126.
12. Emerit J, Chaudiere J: Free radicals and lipid peroxidation in cell pathology; in Miquel J, Quintanilha A, Weber H (eds): Handbook of Free Radicals and Antioxidants in Biomedicine. Boca Raton, CRC Press, 1998.
13. Bergmeyer HU, Brent E: LDH activity; in Bergmeyer HU (ed): Methods of Enzymatic Analysis. New York, Academic Press, 1974, pp 574-579.
14. Cai J, Nelson KC, Wu M, Sternberg P Jr, Jones DP: Oxidative damage and protection of the RPE. Prog Retin Eye Res 2000;19:205-221.
15. Trevithick JR, Dzialoszynski T: Endogenous superoxide-like species and antioxidant activity in ocular tissues detected by luminol luminescence. Biochem Mol Biol Int 1997;4:695-705.
16. Wood JP, Osborne NN: Induction of apoptosis in cultured human retinal pigmented epithelial cells: the effect of protein kinase C activation and inhibition. Neurochem Int 1997;1:261-273.
17. Chida M, Suzuki K, Nakanishi-Ueda T, Yasuhara H, Koide R, Armstrong D: In vitro testing of antioxidants and biochemical end-points in bovine retinal tissue. Ophthalmic Res 1999;31:407-412.
18. Miquel J, Weber HU: Aging and increased oxidation of the sulfur pool; in Viña J (ed): Glutathione Metabolism and Physiological Functions. Boca Raton, CRC Press, 1990, pp 187-192.
19. Miquel J, Ferrandiz ML, De Juan E, Sevilla I, Martínez M: N-Acetylcysteine protects against age-related decline of oxidative phosphorylation in liver mitochondria. Eur J Pharmacol Env Toxicol Pharmacol 1995;292:333-335.
20. De la Fuente M, Ferrandez MD, Del Rio M, Burgos MS, Miquel J: Enhancement of leukocyte functions in aged mice supplemented with the antioxidant thioproline. Mech Ageing Dev 1998;104:213-225.
21. Martínez Banaclocha M, Martinez N: N-acetylcysteine elicited an increase in cytochrome c oxidase activity in mice synaptic mitochondria. Brain Res 1999;842:249-251.
22. Katz ML, Robison WG: Nutritional influences on autoxidation, lipofuscin accumulation, and aging; in Johnson JE, Walford R, Harman D, Miquel J (eds): Free Radicals, Ageing and Degenerative Diseases. New York, Alan R. Liss, 1986, pp 221-259.