Nuclear localization of phosphorylated c-Myc protein in human tumor cells

European Journal of Histochemistry

Volumn 46, Issue 4, 2002, Pages 377-380

  Soldani, C ^a   Bottone, M G ^a   Biggiogera, M ^a,c   Alpini, C ^b   Scovassi, A I ^c   Martin, T ^d   Pellicciari, C ^a,c  

^a UNIVERSITY OF PAVIA   (Italy)

^b FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

^c CNR   (Italy)

^d NONE

Author keywords

C Myc; Fibrillarin; Fluorescence microscopy; Immunocytochemistry; Nuclear bodies; Nucleolus

Indexed keywords

FIBRILLARIN; MYC PROTEIN; NUCLEAR PROTEIN; PROTEIN SC 35; RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL NUCLEUS; CELL NUCLEUS INCLUSION BODY; CELL STRUCTURE; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; HELA CELL; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; NUCLEOLUS; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTO ONCOGENE; TUMOR CELL CULTURE;

EID: 0036973131     PISSN: 1121760X     EISSN: None     Source Type: Journal    
DOI: 10.4081/1750     Document Type: Article

References (21)

1. Alexandrova N., Niklinski J., Bliskovsky V., Otterson G.A., Blake M., Kaye F.J., and Zajac-Kaye M.: The N-terminal domain of c-Myc associates with α-tubulin and microtubules in vivo and in vitro. Mol. Cell. Biol. 15, 5188-5195, 1995.
2. Amati B., Frank S.R., Donjerkovic D., and Taubert S.: Function of the c-myc oncoprotein in chromatin remodeling and transcription. Biochim. Biophys. Acta 1471, M135-M145, 2001.
3. Andersen J.S., Lyon C.E., Fox A.H., Leung A.K.L., Lam Y.W., Steen H., Mann M., and Lamond A.I.: Directed proteomic analysis of the human nucleolus. Curr. Biol. 12, 1-11, 2002.
4. Bernhard W.: A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res. 27, 250-265, 1969.
5. Bloch D.L., Chiche J-D., Orth D., De la Monte S., Rosenzweig, A., and Bloch K.D.: Structural and functional heterogeneity of nuclear bodies. Mol. Biol. Cell 19, 4423-4430, 1999.
6. Clevenger C.V., Epstein A.L., and Bauer K.D.: Modulation of the nuclear antigen p105 as a function of cell-cycle progression. J. Cell Physiol. 130, 336-343, 1987.
7. Flinn E.M., Busch C.M., and Wright A.P.: myc boxes, which are conserved in myc family proteins, are signals for protein degradation via the proteasome. Mol. Cell. Biol. 18, 5961-5969, 1998.
8. Gregory M.A., and Hann S.R.: c-Myc proteolysis by the ubiquitin-proteasome pathway: stabilization of c-Myc in Burkitt's lymphoma cells. Mol. Cell. Biol. 20, 2423-2435, 2000.
9. Henriksson M., Klasson M., Ingvarsson S., Koskinen P., Sumegi J., Klein G., and Thyberg J.: Elevated expression of c-myc and N-myc produces distinct changes in nuclear fine structure and chromatin organization. Oncogene 3, 597-593, 1988.
10. Koskinen P.J., Sistonen L., Evan G., Morimoto R., and Alitalo K.: Nuclear colocalization of cellular and viral myc proteins with HSP70 in myc-overexpressing cells. J. Virol. 65, 842-851, 1991.
11. Lam Y.W., Lyon C.E., and Lamond A.I.: Large-scale isolation of Cajal Bodies from HeLa cells. Mol. Biol. Cell 13, 2461-2473, 2002.
12. Lutz W., Leon J., and Eilers M.: Contributions of Myc to tumorigenesis. Biochim Biophys Acta, 1602, 61-71, 2002.
13. Malatesta M., Zancanaro C., Martin T.E, Chan E.K.L., Amalric F., Lührmann R., Vogel P., and Fakan S.: Is the coiled body involved in nucleolar functions? Exp Cell Res. 211, 415-419, 1994.
14. Martin T.E., and Okamura C.S.: Immunocytochemistry of nuclear hnRNP complexes. In: Busch H. (ed) The Cell Nucleus, Vol. 9, Academic Press, New York, pp. 119-144, 1981.
15. Medina F.J., Cerdido A., and de Carcer G.: The functional organization of the nucleolus in proliferating plant cells. Eur. J. Histochem. 44, 117-131, 2000.
16. Nasi S., Ciarapica R., Jucker R., Rosati J., and Soucek L.: Making decision through Myc. FEBS Lett. 490, 153-162, 2001.
17. Niklinski J., Claassen G., Meyers C., Gregory M.A., Allegra C.J., Kaye F.J., Hann S.R., and Zajac-Kaye M.: Disruption of Myc-tubulin interaction by hyperphosphorylation of c-Myc during mitosis or by constitutive hyperphosphorylation of mutant c-Myc in Burkitt's lymphoma. Mol. Cell. Biol. 20, 5276-5284, 2000.
18. Noguchi K., Kitanaka C., Yamana H., Kokubu A., Mochizuki T., and Kuchino Y.: Regulation of c-Myc through phosphorylation at Ser-62 and Ser-71 by c-Jun N-terminal kinase. J. Biol. Chem. 274, 32580-32587, 1999.
19. Pellicciari C., Bottone M.G., Scovassi A.I., Martin T.E., and Biggiogera M.: Rearrangement of nuclear ribonucleoproteins and extrusion of nucleolus-like bodies during apoptosis induced by hypertonic stress. Eur. J. Histochem. 44, 247-254, 2000.
20. Salghetti S.E., Kim S.Y., and Tansey W.P.: Destruction of Myc by ubiquitin-mediated proteolysis: cancer-associated and transforming mutations stabilize Myc. EMBO J. 18, 717-726, 1999.
21. Spector D.L., Watt R.A., and Sullivan N.F.: The v- and c-myc oncogene proteins colocalize in situ with small nuclear ribonucleoprotein particles. Oncogene 1, 5-12, 1987.