Revisiting electroblotting of immobilized pH gradient gels: A new protocol for studying post-translational modification of proteins

Electrophoresis

Volumn 23, Issue 24, 2002, Pages 4067-4071

  Poland, Julia ^a   Böhme, Axel ^a   Schubert, Kathrin ^a   Sinha, Pranav ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Electrically assisted rehydration; Electroblotting; Erythropoietin; Fabric reinforced gels; Immobilized pH gradient; Net Fix; Proteome analysis; Proteomics

Indexed keywords

ELECTROPHORESIS; MOLECULAR BIOLOGY; PH; PROTEINS;

ELECTRICALLY ASSISTED REHYDRATION; ELECTROBLOTTING; ERYTHROPOIETIN; FABRIC-REINFORCED GEL; IMMOBILIZED PH GRADIENT; NET-FIX; PH GRADIENTS; POST-TRANSLATIONAL MODIFICATIONS; PROTEOME ANALYSIS; PROTEOMICS;

GELS;

ISOPROTEIN; PROTEOME; RECOMBINANT ERYTHROPOIETIN;

ANALYTIC METHOD; ARTICLE; CHEMOLUMINESCENCE; CONTROLLED STUDY; DEVICE; ELECTRIC CURRENT; ELECTROBLOTTING; ELECTROPHORETIC MOBILITY; HYDRATION; IMMUNOBLOTTING; ISOELECTRIC FOCUSING; LABORATORY TEST; PERMEABILITY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN MODIFICATION; PROTEIN PROCESSING; SEPARATION TECHNIQUE; TEST STRIP; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0036965256     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200290022     Document Type: Article

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