The cysteine desulfurase IscS is required for synthesis of all five thiolated nucleosides present in tRNA from Salmonella enterica serovar typhimurium

Journal of Bacteriology

Volumn 184, Issue 24, 2002, Pages 6830-6835

  Nilsson, Kristina ^a   Lundgren, Hans K ^a   Hagervall, Tord G ^a   Björk, Glenn R ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DERIVATIVE; BACTERIAL RNA; CYSTATHIONINE GAMMA LYASE; CYTIDINE DERIVATIVE; HISTIDINE; NUCLEOSIDE DERIVATIVE; TRANSFER RNA; URIDINE DERIVATIVE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; CELL GROWTH; CONTROLLED STUDY; DELETION MUTANT; ESCHERICHIA COLI; GENE DELETION; GENE MUTATION; INCUBATION TIME; NONHUMAN; NUCLEIC ACID SYNTHESIS; PRIORITY JOURNAL; PROTEIN FUNCTION; RIBOSOMAL FRAMESHIFTING; SALMONELLA ENTERICA;

CARBON-SULFUR LYASES; MUTATION; RNA, BACTERIAL; SALMONELLA TYPHIMURIUM; THIONUCLEOTIDES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA; PROKARYOTA; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

EID: 0036955843     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.24.6830-6835.2002     Document Type: Article

References (30)

1. Ajitkumar, P., and J. D. Cherayil. 1988. Thionucleosides in transfer ribonucleic acid: diversity, structure, biosynthesis, and function. Microbiol. Rev. 52:103-113.
2. Auffinger, P., E. Westhof. 1998. Location and distribution of modified nucleotides in tRNA, p. 569-576. In H. Grosjean and R. Benne (ed.), Modification and editing of RNA. ASM Press, Washington, D.C.
3. Bartolome, B., Y. Jubete, E. Martinez, and F. de la Cruz. 1991. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives, Gene 102:75-78.
4. Begley, T. P., J. Xi, C. Kinsland, S. Taylor, and F. McLafferty. 1999. The enzymology of sulfur activation during thiamin and biotin biosynthesis. Curr. Opin. Chem. Biol. 3:623-629.
5. Bertani, G. 1951. Studies on lysogenesis. J. Bacteriol. 2:293-300.
6. Björk, G. R. 1980. A novel link between the biosynthesis of aromatic amino acids and transfer RNA modification in Escherichia coli. J. Mol. Biol. 140:391-410.
7. Buck, M., J. A. McCloskey, B. Basile, and B. N. Ames. 1982. cis-2-Methylthio-ribosylzeatin (N-6-(4-hydroxyisopentenyl)-2-methylthioadenosine) is present in the transfer RNA of Salmonella typhimurium, but not Escherichia coli. Nucleic Acids Res. 10:5649-5662.
8. Davis, W., D. Botstein, and J. R. Roth. 1980. Advanced bacterial genetics: a manual for genetic engineering. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
9. Emilsson, V., and C. G. Kurland. 1990. Growth rate dependence of transfer RNA abundance in Escherichia coli. EMBO J. 9:4359-4366.
10. 6A) present next to the anticodon contributes to the decoding efficiency of the tRNA. J. Bacteriol. 177:1967-1975.
11. Esberg, B., H. C. Leung, H. C. Tsui, G. R. Björk, and M. E. Winkler. 1999. Identification of the miaB gene, involved in methylthiolation of isopentenylated A37 derivatives in the tRNA of Salmonella typhimurium and Escherichia coli. J. Bacteriol. 181:7256-7265.
12. Fabien, P., G. R. Björk, M. Fontecave, and M. Atta. 2002. Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein. J. Biol. Chem. 277:13367-13370.
13. Gehrke, C. W., and K. C. Kuo. 1990. Ribonucleoside analysis by reversed-phase high performance liquid chromatography, p. A3-A71. In C. W. Gehrke and K. C. T. Kuo (ed.), Chromatography and modification of nucleosides, part A: analytical methods for major and modified nucleosides. Elsevier, Amsterdam, The Netherlands.
14. Gehrke, C. W., K. C. Kuo, R. A. McCune, K. O. Gerhardt, and P. F. Agris. 1982. Quantitative enzymatic hydrolysis of tRNAs: reversed-phase high-performance liquid chromatography of tRNA nucleosides. J. Chromatogr. 230:297-308.
15. 4U in Escherichia coli tRNA. J. Biol. Chem. 275:10727-10730.
16. 4U, thiamine, and NAD. J. Biol. Chem. 275:20096-20103.
17. McCloskey, J. A., D. E. Graham, S. Zhou, P. F. Crain, M. Ibba, J. Konisky, D. Soil, and G. J. Olsen. 2001. Post-transcriptional modification in archaeal tRNAs: identities and phylogenetic relations of nucleotides from mesophilic and hyperthermophilic Methanococcales. Nucleic Acids Res. 29:4699-4706.
18. 4U biosynthesis, may be a sulfurtransferase that proceeds through a persulfide intermediate. J. Biol. Chem. 275:8283-8286.
19. Qian, Q., J. N. Li, H. Zhao, T. G. Hagervall, P. J. Farabaugh, and G. R. Björk. 1998. A new model for phenotypic suppression of frameshift mutations by mutant tRNAs. Mol. Cell 1:471-482.
20. 4U in its transfer ribonucleic acid. J. Bacteriol. 128:671-672.
21. Rozenski, J., P. F. Crain, and J. A. McCloskey. 1999. The RNA modification database: 1999 update. Nucleic Acids Res. 27:196-197.
22. Schmieger, H. 1972. Phage P22 mutants with increased or decreased transduction abilities. Mol. Gen. Genet. 119:75-88.
23. Skovran, E., and D. M. Downs. 2000. Metabolic defects caused by mutations in the isc gene cluster in Salmonella enterica serovar Typhimurium: implications for thiamine synthesis. J. Bacteriol. 182:3896-3903.
24. Sullivan, M. A., J. F. Cannon, F. H. Webb, and R. M. Bock. 1985. Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine. J. Bacteriol. 161:368-376.
25. Takahashi, Y., and M. Nakamura. 1999. Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli. J. Biochem. (Tokyo) 126:917-926.
26. Urbonavicius, J., Q. Qian, J. M. Durand, T. G. Hagervall, and G. R. Björk. 2001. Improvement of reading frame maintenance is a common function for several tRNA modifications. EMBO J. 20:4863-4873.
27. Vogel, H. J., and D. M. Bonner. 1956. Acetylornithinase of Escherichia coli: partial purification and some properties. J. Biol. Chem. 218:97-106.
28. Zheng, L., V. L. Cash, D. H. Flint, and D. R. Dean. 1998. Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii. J. Biol. Chem. 273:13264-13272.
29. Zheng, L., R. H. White, V. L. Cash, and D. R. Dean. 1994. Mechanism for the desulfurization of L-cysteine catalyzed by the nifS gene product. Biochemistry 33:4714-4720.
30. Zheng, L., R. H. White, V. L. Cash, R. F. Jack, and D. R. Dean. 1993. Cysteine desulfurase activity indicates a role for NifS in metallocluster biosynthesis. Proc. Natl. Acad. Sci. USA 90:2754-2758.