A mechanism of the thearubigin fraction of black tea (Camellia sinensis) extract protecting against the effect of tetanus toxin

Journal of Toxicological Sciences

Volumn 27, Issue 5, 2002, Pages 441-447

  Satoh, Eiki ^a   Ishii, Toshiaki ^a   Shimizu, Yoshio ^a   Sawamura, Shin ichi ^b   Nishimura, Masakazu ^a  

^a OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

^b 421 0516   (Japan)

Author keywords

Black tea; Phrenic nerve diaphragm; Specific binding; Synaptosomal membrane; Tetanus toxin; Thearubigin fraction

Indexed keywords

BLACK TEA EXTRACT; TETANUS TOXIN; THEARUBIGIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BINDING KINETICS; BRAIN CORTEX; BRAIN SYNAPTOSOME; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DRUG BINDING; FEMALE; IN VITRO STUDY; INHIBITION KINETICS; INTERNALIZATION; MALE; MEMBRANE BINDING; MOUSE; NEUROMUSCULAR BLOCKING; NEUROTRANSMITTER RELEASE; NONHUMAN; PHRENIC NERVE; SYNAPTIC MEMBRANE; TIC; TOXIN ANALYSIS;

ANIMALIA; CAMELLIA; CAMELLIA SINENSIS;

EID: 0036947752     PISSN: 03881350     EISSN: 18803989     Source Type: Journal    
DOI: 10.2131/jts.27.441     Document Type: Article

References (17)

1. Bradford, M.M. (1976): Rapid and sensitive method for quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
2. Habermann, E., Dreyer, F. and Bigalke, H. (1980): Tetanus toxin blocks the neuromuscular transmission in vitro like botulinum A toxin. NaunynSchmiedeberg's Arch. Pharmacol., 311, 33-40.
3. Hajós, F. (1975): An improved method for the preparation of synaptosomal fraction in high purity. Brain Res., 93, 485-489.
4. Hazarika, M., Chakravarty, S.K. and Mahanta, P.K. (1984): Studies on thearubigin pigments in black tea manufacturing systems. J. Sci. Food Agric., 35, 1208-1218.
5. Henderson, D.A. (1999): The looming threat of bioterrorism. Science, 283, 1279-1282.
6. Kondo, H., Shimizu, T., Kubonoya, M., Izumi, N., Takahashi, M. and Sakaguchi, G. (1984): Titration of botulinum toxins for lethal toxicity by intravenous injection into mice. Jpn. J. Med. Sci. Biol., 37, 131-135.
7. Li, L. and Singh, B.R. (1999): In vitro translation of type A clostridium botulinum neurotoxin heavy chain and analysis of its binding to rat synaptosomes. J. Protein Chem., 18, 89-95.
8. Montecucco, C. and Schiavo, G. (1995): Structure and function of tetanus and botulinum neurotoxins. Q. Rev. Biophys., 28, 423-472.
9. Pellizzari, R., Rossetto, O., Schiavo, G. and Montecucco, C. (1999): Tetanus and botulinum neurotoxins: Mechanism of action and therapeutic uses. Phil. Trans. R. Soc. Lond. B, 354, 259-268.
10. Roberts, E.A.H. (1958): The phenolic substances of manufactured tea. II. Their origin as enzymic oxidation products in fermentation. J. Sci. Food Agric., 9, 212-216.
11. Satoh, E., Ishii, T., Shimizu, Y., Sawamura, S. and Nishimura, M. (2001a): Black tea extract, thearubigin fraction, counteract the effects of botulinum neurotoxins in mice. Br. J. Pharmacol., 132, 797-798.
12. Satoh, E., Ishii, T., Shimizu, Y., Sawamura, S. and Nishimura, M. (2001b): Black tea extract, thearubigin fraction, counteracts the effect of tetanus toxin in mice. Proc. Soc. Exp. Biol. Med., 226, 577-580.
13. Satoh, E., Ishii, T., Shimizu, Y., Sawamura, S. and Nishimura, M. (2002): The mechanism underlying the protective effect of the thearubigin fraction of black tea (Camellia sinensis) extract against the neuromuscular blocking action of botulinum neurotoxins. Pharmacol. Toxicol., 90, 199-202.
14. Simpson, L.L., Coffield, J.A. and Bakry, N. (1993): Chelation of zinc antagonizes the neuromuscular blocking properties of the seven serotypes of botulinum neurotoxin as well as tetanus toxin. J. Pharmacol. Exp. Ther., 267, 720-727.
15. Singleton, V.L. and Rossi, J.A.Jr. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult., 16, 144-158.
16. Steel, R.G.D. and Torrie, J.H. (1980): Principles and Procedures of Statistics, 2nd ed. McGraw-Hill Book Company, Inc., New York, NY.
17. Xie, B., Shi, H., Chen, Q. and Ho, C.-T. (1993): Antioxidant properties of fractions and polyphenol constituents from green, oolong and black teas. Proc. Natl. Sci. Counc. Repub. China B, 17, 77-84.