Titin: An endosarcomeric protein that modulates myocardial stiffness in DCM

Journal of Cardiac Failure

Volumn 8, Issue 6 SUPPL., 2002, Pages

  Wu, Yiming ^a   LaBeit, Siegfried ^b   Lewinter, Martin M ^c   Granzier, Henk ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

Cardiomyopathy; Diastole; Length dependent activation; Myocardium; Phosphorylation; Titin

Indexed keywords

CONNECTIN; MYOSIN ADENOSINE TRIPHOSPHATASE;

CONFERENCE PAPER; HEART DILATATION; HEART DISEASE; HEART MUSCLE; HUMAN; MYOFILAMENT; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SARCOMERE;

EID: 0036947533     PISSN: 10719164     EISSN: None     Source Type: Journal    
DOI: 10.1054/jcaf.2002.129278     Document Type: Conference Paper

References (46)

1. Labeit S, Kolmerer B: Titins: Giant proteins in charge of muscle ultrastructure and elasticity. Science 1995;270:293-296
2. Granzier HL, Irving TC: Passive tension in cardiac muscle: Contribution of collagen, titin, microtubules, and intermediate filaments. Biophys J 1995;68:1027-1044
3. Gregorio CC, Trombitas K, Centner T, Kolmerer B, Stier G, Kunke K, Suzuki K, Obermayr F, Herrmann B, Granzier H, Sorimachi H, Labeit S: The NH2 terminus of titin spans the Z-disc: Its interaction with a novel 19-kD ligand (T-cap) is required for sarcomeric integrity. J Cell Biol 1998;143:1013-1027
4. Obermann WM, Gautel M, Weber K, Furst DO: Molecular structure of the sarcomeric M band: Mapping of titin and myosin binding domains in myomesin and the identification of a potential regulatory phosphorylation site in myomesin. EMBO J 1997;16:211-220
5. Gregorio CC, Granzier H, Sorimachi H, Labeit S: Muscle assembly: A titanic achievement? Curr Opin Cell Biol 1999;11:18-25
6. Trombitas K, Granzier H: Actin removal from cardiac myocytes shows that near Z line titin attaches to actin while under tension. Am J Physiol 1997;273:C662-C670
7. Trombitas K, Jin JP, Granzier H: The mechanically active domain of titin in cardiac muscle. Circ Res 1995;77:856-861
8. Granzier H, Helmes M, Trombitas K: Nonuniform elasticity of titin in cardiac myocytes: A study using immunoelectron microscopy and cellular mechanics. Biophys J 1996;70:430-442
9. Granzier H, Kellermayer M, Helmes M, Trombitas K: Titin elasticity and mechanism of passive force development in rat cardiac myocytes probed by thin-filament extraction. Biophys J 1997;73:2043-2053
10. Helmes M, Trombitas K, Centner T, Kellermayer M, Labeit S, Linke WA, Granzier H: Mechanically driven contour-length adjustment in rat cardiac titin's unique N2B sequence: Titin is an adjustable spring. Circ Res 1999;84:1339-1352
11. Linke WA, Rudy DE, Centner T, Gautel M, Witt C, Labeit S, Gregorio CC: I-band titin in cardiac muscle is a three-element molecular spring and is critical for maintaining thin filament structure. J Cell Biol 1999;146:631-644
12. Trombitas K, Freiburg A, Centner T, Labeit S, Granzier H: Molecular dissection of N2B cardiac titin's extensibility. Biophys J 1999;77:3189-3196
13. Wang K: Cytoskeletal matrix in striated muscle: The role of titin, nebulin and intermediate filaments. Adv Exp Med Biol 1984;170:285-305
14. Cazorla O, Wu Y, Irving TC, Granzier H: Titin-based modulation of calcium sensitivity of active tension in mouse skinned cardiac myocytes. Circ Res 2001;88:1028-1035
15. Helmes M, Trombitas K, Granzier H: Titin develops restoring force in rat cardiac myocytes. Circ Res 1996;79:619-626
16. Freiburg A, Trombitas K, Hell W, Cazorla O, Fougerousse F, Centner T, Kolmerer B, Witt C, Beckmann JS, Gregorio CC, Granzier H, Labeit S: Series of exon-skipping events in the elastic spring region of titin as the structural basis for myofibrillar elastic diversity. Circ Res 2000;86:1114-1121
17. Cazorla O, Freiburg A, Helmes M, Centner T, McNabb M, Wu Y, Trombitas K, Labeit S, Granzier H: Differential expression of cardiac titin isoforms and modulation of cellular stiffness. Circ Res 2000;86:59-67
18. Trombitas K, Redkar A, Centner T, Wu Y, Labeit S, Granzier H: Extensibility of isoforms of cardiac titin: Variation in contour length of molecular subsegments provides a basis for cellular passive stiffness diversity. Biophys J 2000;79:3226-3234
19. Trombitas K, Greaser M, French G, Granzier H: PEVK extension of human soleus muscle titin revealed by immunolabeling with the anti-titin antibody 9D10. J Struct Biol 1998;122:188-196
20. Trombitas K, Wu Y, Labeit D, Labeit S, Granzier H: Cardiac titin isoforms are coexpressed in the half-sarcomere and extend independently. Am J Physiol Heart Circ Physiol 2001;281:1793-1799
21. Wu Y, Cazorla O, Labeit D, Labeit S, Granzier H: Changes in titin and collagen underlie diastolic stiffness diversity of cardiac muscle. J Mol Cell Cardiol 2000;32:2151-2162
22. Mayans O, van der Ven PF, Wilm M, Mues A, Young P, Furst DO, Wilmanns M, Gautel M: Structural basis for activation of the titin kinase domain during myofibrillogenesis. Nature 1998;395:863-869
23. Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S: Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol 2001;306:717-726
24. Somerville LL, Wang K: In vivo phosphorylation of titin and nebulin in frog skeletal muscle. Biochem Biophys Res Commun 1987;147:986-992
25. Strang KT, Sweitzer NK, Greaser ML, Moss RL: Beta-adrenergic receptor stimulation increases unloaded shortening velocity of skinned single ventricular myocytes from rats. Circ Res 1994;74:542-549
26. Gautel M, Leonard K, Labeit S: Phosphorylation of KSP motifs in the C-terminal region of titin in differentiating myoblasts. EMBO J 1993;12:3827-3834
27. Sebestyen MG, Wolff JA, Greaser ML: Characterization of a 5.4 kb cDNA fragment from the Z-line region of rabbit cardiac titin reveals phosphorylation sites for proline-directed kinases. J Cell Sci 1995;108:3029-3037
28. Yamasaki R, Wu Y, McNabb M, Greaser M, Labeit S, Granzier H: Protein kinase A phosphorylates titin's cardiac-specific N2B domain and reduces passive tension in rat cardiac myocytes. Circ Res 2002;90:1181-1188
29. Freeman K, Lerman I, Kranias EG, Bohlmeyer T, Bristow MR, Lefkowitz RJ, Iaccarino G, Koch WJ, Leinwand LA: Alterations in cardiac adrenergic signaling and calcium cycling differentially affect the progression of cardiomyopathy. J Clin Invest 2001;107:967-974
30. Fuchs F, Smith SH: Calcium, cross-bridges, and the Frank-Starling relationship. News Physiol Sci 2001;16:5-10
31. Fuchs F, Wang YP: Sarcomere length versus interfilament spacing as determinants of cardiac myofilament Ca2+ sensitivity and Ca2+ binding. J Mol Cell Cardiol 1996;28:1375-1383
32. McDonald KS, Moss RL: Osmotic compression of single cardiac myocytes eliminates the reduction in Ca2+ sensitivity of tension at short sarcomere length. Circ Res 1995;77:199-205
33. Granzier HL, Wang K: Passive tension and stiffness of vertebrate skeletal and insect flight muscles: The contribution of weak cross-bridges and elastic filaments. Biophys J 1993;65:2141-2159
34. Granzier HL, Wang K: Interplay between passive tension and strong and weak binding crossbridges in insect indirect flight muscle. A functional dissection by gelsolin-mediated thin filament removal. J Gen Physiol 1993;101:235-270
35. Horowits R, Podolsky RJ: Thick filament movement and isometric tension in activated skeletal muscle. Biophys J 1988;54:165-171
36. Horowits R, Podolsky RJ: The positional stability of thick filaments in activated skeletal muscle depends on sarcomere length: Evidence for the role of titin filaments. J Cell Biol 1987;105:2217-2223
37. Granzier H, Helmes M, Cazorla O, McNabb M, Labeit D, Wu Y, Yamasaki R, Redkar A, Kellermayer M, Labeit S, Trombitas K: Mechanical properties of titin isoforms. Adv Exp Med Biol 2000;481:283-300
38. Bell SP, Nyland L, Tischler MD, McNabb M, Granzier H, LeWinter MM: Alterations in the determinants of diastolic suction during pacing tachycardia. Circ Res 2000;87:235-240
39. Sorimachi H, Ono Y, Suzuki K: Skeletal muscle-specific calpain, p94, and connectin/titin: Their physiological functions and relationship to limb-girdle muscular dystrophy type 2A. Adv Exp Med Biol 2000;481:383-395
40. Hein S, Kostin S, Heling A, Maeno Y, Schaper J: The role of the cytoskeleton in heart failure. Cardiovasc Res 2000;45:273-278
41. Hein S, Schaper J: Pathogenesis of dilated cardiomyopathy and heart failure: Insights from cell morphology and biology. Curr Opin Cardiol 1996;11:293-301
42. Hein S, Scholz D, Fujitani N, Rennollet H, Brand T, Friedl A, Schaper J: Altered expression of titin and contractile proteins in failing human myocardium. J Mol Cell Cardiol 1994;26:1291-1306
43. Morano I, Hadicke K, Grom S, Koch A, Schwinger RH, Bohm M, Bartel S, Erdmann E, Krause EG: Titin, myosin light chains and C-protein in the developing and failing human heart. J Mol Cell Cardiol 1994;26:361-368
44. Vahl CF, Timek T, Bonz A, Kochsiek N, Fuchs H, Schaffer L, Rosenberg M, Dillmann R, Hagl S: Myocardial length-force relationship in end stage dilated cardiomyopathy and normal human myocardium: Analysis of intact and skinned left ventricular trabeculae obtained during 11 heart transplantations. Basic Res Cardiol 1997;92:261-270
45. Holubarsch C, Ruf T, Goldstein DJ, Ashton RC, Nickl W, Pieske B, Pioch K, Ludemann J, Wiesner S, Hasenfuss G, Posival H, Just H, Burkhoff D: Existence of the Frank-Starling mechanism in the failing human heart. Investigations on the organ, tissue, and sarcomere levels. Circulation 1996;94:683-689
46. Liversage AD, Holmes D, Knight PJ, Tskhovrebova L, Trinick J: Titin and the sarcomere symmetry paradox. J Mol Biol 2001;305:401-409