Characterization of microbial community in nitrogen removal process of metallurgic wastewater by PCR-DGGE

Water Science and Technology

Volumn 46, Issue 11-12, 2002, Pages 93-98

  Yoshie, S ^a   Noda, N ^a   Miyano, T ^a   Tsuneda, S ^a   Hirata, A ^a   Inamori, Y ^b  

^a WASEDA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

Author keywords

16S rDNA; Denitrification; Hypersaline; Metallurgic wastewater; Microbial community; PCR DGGE

Indexed keywords

AMMONIA; BIOREACTORS; DENITRIFICATION; ELECTROPHORESIS; INDUSTRIAL WASTES; MICROORGANISMS; NITRIC ACID; NITROGEN REMOVAL; SODIUM CHLORIDE;

MICROBIAL COMMUNITY;

WASTEWATER TREATMENT;

AMMONIA; DNA 16S; NITRATE; NITRIC ACID; NITRITE; NITROGEN; SODIUM CHLORIDE; SODIUM SULFATE;

BIOREACTOR; DENITRIFICATION; MINERAL PROCESSING; PACKED BED; WASTEWATER;

ALCALIGENES; BACTERIAL FLORA; BIOREACTOR EQUIPMENT; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CONFERENCE PAPER; DENATURING GRADIENT GEL ELECTROPHORESIS; DENITRIFICATION; DNA SEQUENCE; FLUIDIZED BED REACTOR; INDUSTRIAL WASTE; IONIC STRENGTH; METAL RECOVERY; METALLURGY; MICROANALYSIS; MICROBIAL IDENTIFICATION; MICROFLORA; NONHUMAN; PACKED BED REACTOR; POLYMERASE CHAIN REACTION; PSEUDOMONAS; SLUDGE; WASTE COMPONENT REMOVAL; WASTE WATER MANAGEMENT; WATER LOADING; WATER TEMPERATURE;

ALCALIGENES; ALCALIGENES DEFRAGRANS; BACTERIA (MICROORGANISMS); PROTEOBACTERIA; PSEUDOMONAS;

EID: 0036946619     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2002.0722     Document Type: Conference Paper

References (6)

1. Hirata, A., Nakamura, Y. and Tsuneda, S. (2001). Biological nitrogen removal from industrial wastewater discharged from metal recovery processes. Wat. Sci. Tech. 44(2-3), 171-180.
2. Kowalchuk, G.A., Stephan, J.R., De Boer, W., Prosser, J.I., Embley, T.M. and Woldendorp, J.W (1998). Analysis of of β-proteobacteria ammmonia-oxidising bacteria in oastal sand dunes using denaturing gradient gel electrophoresis and sequencing of PCR amplified 16SrDNA fragments. Appl. Environ. Microbiol., 63, 1489-1497.
3. Muyzer, G., Brinkhoff, T., Nubel, U., Santegoeds, C., Schafer, H. and Wawer C. (1998). Denaturing gradient gel electrophoresis (DGGE) in microbial ecology. In: Molecular Microbiology Ecology Manual, A.D.L. Akkermans, J.D. v. Elsas and F.J. De Bruijin (eds), Kluwer Academic Publishers, Dordrecht, The Netherlands, 3.4.4., 1-27.
4. Muyzer, G., De Waal, E.C. and Uitterlinden, A.G. (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes encoding for 16SrDNA. Appl. Environ. Microbiol., 59, 695-700.
5. Myers, R.M., Sheffield, V.C. and Cox, D.R. (1989). Mutation detection by PCR, GC-clamps, and denaturing gradient gel electrophoresis. InL PCR-Technology - Principles and Applications for DNA Amplification, Erlich, H.A. (ed), Stockton Press, New York, pp. 71-88.
6. Schuch, R., Ghensicke, R., Merkel, K. and Winter, J. (2000). Nitrogen and DOC removal from wastewater streams of the metal-working industry. Wat. Res., 34, 295-303.