Cryopreservation of reduced cytochrome C for determination of N-formyl-methionyl-leucyl-phenylalanine-stimulated superoxide anion production in human whole blood

European Journal of Applied Physiology

Volumn 87, Issue 4-5, 2002, Pages 365-372

  Chouker A ^a   Martignoni, A ^a   DaSilva, L ^a   Diem, H ^a   Christ, F ^a   Peter, K ^a   Thiel, M ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

Cryopreservation; Poymorphonuclear leucocytes; Superoxide anions; Sytochrome C

Indexed keywords

BACTERIAL PROTEIN; CYTOCHROME C; FORMYLMETHIONYLLEUCYLPHENYLALANINE; OXIDIZING AGENT; REACTIVE OXYGEN METABOLITE; SUPEROXIDE; TRIPEPTIDE;

AEROSPACE MEDICINE; ANALYTIC METHOD; ARTICLE; BLOOD SAMPLING; CELL ACTIVATION; CELL ISOLATION; CELL STIMULATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYOPRESERVATION; GRANULOCYTE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; MALE; MEASUREMENT; NORMAL HUMAN; PHAGOCYTE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STABILITY; RELIABILITY; SPECTROPHOTOMETRY;

CELL SEPARATION; CRYOPRESERVATION; CYTOCHROME C GROUP; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; LEUKOCYTE COUNT; N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE; NEUTROPHILS; OXIDATION-REDUCTION; SUPEROXIDES; TIME FACTORS;

EID: 0036942696     PISSN: 14396319     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00421-002-0650-0     Document Type: Article

References (21)

1. Arthur MJ, Kowalski Saunders P, Gurney S, Tolcher R, Bull FG, Wright R (1987) Reduction of ferricytochrome C may underestimate superoxide production by monocytes. J Immunol Meth 98:63-69
2. Bellavite P, Dri P, Della Bianca V, Serra MC (1983) The measurement of superoxide anion production by granulocytes in whole blood. A clinical test for the evaluation of phagocyte function and serum opsonic capacity. Eur J Clin Invest 13:363-368
3. Bellavite P, Guarani P, Biasi D, Carletto A, Trevisan MT, Caramaschi P, Bambara LM, Corrocher R (1995) Correlations between the intensity of fMLP-dependent respiratory burst and cellular fatty acid composition in human neutrophils. Br J Haematol 89:271-276
4. Bielefeldt OH, Babiuk LA (1984) In vitro generation of hydrogen peroxide and of superoxide anion by bovine polymorphonuclear neutrophilic granulocytes, blood monocytes, and alveolar macrophages. Inflammation 8:251-275
5. Boyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest [Suppl] 21:77-89
6. Choukèr A, Demetz F, Martignoni A, Setzter F, Smith L, Christ F, Thiel M (2000) Effects of high altitude and physical exercise on polymorphonuclear leukocytes (PMNL). In: West JB (ed) Conference Proceedings. High Altitude, Medicine and Biology. Liebertpub, La Jolla, p 237
7. Choukèr A, Thiel M, Baranov V, Meshkov D, Kotov A, Peter K, Messmer K, Christ F (2001) Simulated microgravity, psychic stress, and immune cells in men: observations during 120-day 6 degrees HDT. J Appl Physiol 90:1736-1743
8. Choukèr A, Smith L, Christ F, Larina I, Nichiporuk I, Baranov V, Bobrovnik E, Pastushkova L, Messmer K, Peter K, Thiel M (2002) Effects of confinement (110 and 240 days) on neuroendocrine stress response and changes of immune cells in men. J Appl Physiol 92:1619-1627
9. Fearon DT, Collins LA (1983) Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J Immunol 130:370-375
10. Grzeskowiak M, Della Bianca V, Cassatella MA, Rossi F (1986) Complete dissociation between the activation of phosphoinositide turnover and of NADPH oxidase by formyl-methionyl-leucyl-phenylalanine in human neutrophils depleted of Ca2 + and primed by subthreshold doses of phorbol 12,myristate 13, acetate. Biochem Biophys Res Commun 135:785-794
11. Hamblin A, Taylor M, Bernhagen J, Shakoor Z, Mayall S, Noble G, McCarthy D (1992) A method of preparing blood leucocytes for flow cytometry which prevents upregulation of leucocyte integrins. J Immunol Meth 146:219-228
12. Markert M, Andrews PC, Babior BM (1984) Measurement of O2-production by human neutrophils. The preparation and assay of NADPH oxidase-containing particles from human neutrophils. Meth Enzymol 105:358-365
13. McNeil CJ, Smith KA, Bellavite P, Bannister JV (1989) Application of the electrochemistry of cytochrome c to the measurement of superoxide radical production. Free Radic Res Commun 7:89-96
14. Richter J (1992) Effect of adenosine analogues and cAMP-raising agents on TNF-, GM-CSF-, and chemotactic peptide-induced degranulation in single adherent neutrophils. J Leukocyte Biol 51:270-275
15. Root RK, Metcalf JA (1977) H2O2 release from human granulocytes during phagocytosis. Relationship to superoxide anion formation and cellular catabolism of H2O2: studies with normal and cytochalasin B- treated cells. J Clin Invest 60:1266-1279
16. Seeds MC, Parce JW, Szejda P, Bass DA (1985) Independent stimulation of membrane potential changes and the oxidative metabolic burst in polymorphonuclear leukocytes. Blood 1:233-240
17. Thiel M, Chambers JD, Choukèr A, Fischer S, Zourelidis C, Bardenheuer HJ, Arfors KE, Peter K (1996) Effect of adenosine on the expression of beta(2) integrins and L- selectin of human polymorphonuclear leukocytes in vitro. J Leukocyte Biol 59:671-682
18. Thomas L (1992) Labor und Diagnose. Medizinische Verlagsge-sellschaft, Marburg, pp 1849-1864
19. Vasquez Vivar J, Hogg N, Pritchard KA Jr, Martasek P, Kalyanaraman B (1997) Superoxide anion formation from lucigenin: an electron spin resonance spin-trapping study. FEBS Lett 403:127-130
20. Wilson E, Olcott MC, Bell RM, Merrill AH Jr, Lambeth JD (1986) Inhibition of the oxidative burst in human neutrophils by sphingoid long-chain bases. Role of protein kinase C in activation of the burst. J Biol Chem 261:12616-12623
21. Yang KD, Hill HR (1991) Neutrophil function disorders: pathophysiology, prevention, and therapy. J Pediatr 119:343-354