Site-directed mutagenesis of a potential catalytic and formyl phosphate binding site and substrate inhibition of N10-formyltetrahydrofolate synthetase

Archives of Biochemistry and Biophysics

Volumn 408, Issue 1, 2002, Pages 137-143

  Leaphart, Adam B ^a   Spencer, H Trent ^b   Lovell, Charles R ^a  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Formyl phosphate intermediate; Ligand binding; N10 Formyltetrahydrofolate synthetase; Substrate inhibition

Indexed keywords

ADENOSINE TRIPHOSPHATE; FORMIC ACID; FORMYL PHOSPHATE; GLUTAMIC ACID; N 10 FORMYLTETRAHYDROFOLATE SYNTHETASE; PHOSPHATE; SERINE; SYNTHETASE; TETRAHYDROFOLIC ACID; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; DISSOCIATION; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME INHIBITION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; BACTERIA, ANAEROBIC; BINDING SITES; CATALYTIC DOMAIN; CLONING, MOLECULAR; FORMATE-TETRAHYDROFOLATE LIGASE; FORMIC ACIDS; KINETICS; MUTAGENESIS, SITE-DIRECTED; ORGANOPHOSPHORUS COMPOUNDS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; TETRAHYDROFOLATES;

EID: 0036938424     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-9861(02)00552-0     Document Type: Article

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