Sensitivity of a novel biotin-free detection reagent (PowerVision+™) for immunohistochemistry

Journal of Histotechnology

Volumn 25, Issue 4, 2002, Pages 247-250

  Petrosyan, Karen ^a   Tamayo, Rosalba ^a   Joseph, Daisy ^a  

^a IMPATH Inc   (United States)

Author keywords

Antigen retrieval; Heat induced epitope retrieval; Immunohistochemistry; Polymeric labeling

Indexed keywords

BIOTIN; ENZYME ANTIBODY; FORMALDEHYDE; PARAFFIN; POLYMER; REAGENT;

ARTICLE; CONJUGATE; CONTROLLED STUDY; DILUTION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INCUBATION TIME; PROTEIN ANALYSIS; STANDARDIZATION; UNITED STATES;

EID: 0036933225     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/014788802794791951     Document Type: Article

References (5)

1. Cartun RW, Pederson CA: An immunocytochemical technique offering increased sensitivity and lowered cost with a streptavidin-horseradish peroxidase conjugate. J Histotechnol 12:273-277, 1989
2. Shi S, Guo J, Cote RJ, Young LL, Hawes D, Shi Y, et al: Sensitivity and detection efficiency of a novel two-step detection system (PowerVision) for immunohistochemistry. Appl Immunohistochem Mol Morphol 7:201-208, 1999
3. Wood GS, Warnke R: Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 10:1196-1201, 1981
4. IMPATH Inc: Immunohistochemistry Standard Operating Procedure, Techmate Procedure for Hematopoietic Antibodies Using the Labeled Streptavidin-Biotin (LSAB) Detection Method, 2002
5. IMPATH Inc: Immunohistochemistry Standard Operating Procedure, Techmate Envision Procedure for General Differentiation Antibodies Using DAKO's Envision™ Detection Reagent, 2002