Sheep adrenal cytochrome b5: Active as a monomer or a tetramer in vivo?

Endocrine Research

Volumn 28, Issue 4, 2002, Pages 485-492

  Lombard, Nicolaas ^a   Swart, Amanda C ^a   Van der Merwe, Marthinus J ^a   Swart, Pieter ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CYTOCHROME B5; DETERGENT; ISOENZYME; LIVER ENZYME; MERCAPTOETHANOL; MONOMER; TETRAMER; TRYPSIN;

ADRENAL GLAND; ANIMAL TISSUE; ANTIBODY PRODUCTION; ANTIGEN PRESENTATION; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; IN VIVO STUDY; LIVER; MEMBRANE BINDING; MICROSOME; MOLECULAR RECOGNITION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN QUATERNARY STRUCTURE; SHEEP; STEROIDOGENESIS;

ADRENAL GLANDS; ANIMALS; BLOTTING, WESTERN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROMES B5; IMMUNE SERA; MICROSOMES; MOLECULAR WEIGHT; PRECIPITIN TESTS; PROGESTERONE; PROTEIN ISOFORMS; PROTEIN STRUCTURE, QUATERNARY; SHEEP; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPSIN;

EID: 0036932862     PISSN: 07435800     EISSN: None     Source Type: Journal    
DOI: 10.1081/ERC-120016827     Document Type: Conference Paper

References (12)

1. Strittmatter, P. In The Enzymes; Boyer, P.D., Lardy, H., Myrback, K., Eds.; Academic Press: New York, 1963; Vol. 8, 114-139.
2. Takemori, S.; Kominami, S. The role of cytochrome P450 in adrenal steroidogenesis. TIBS 1984, 393-396.
3. 5 in adrenal microsomal steroidogenesis. Steroid. Biol. and Molec. Biol. 1992, 42, 57-64.
4. 5 on progesterone metabolism in the ovine adrenal. Endocrine. Res. 1995, 21, 297-306.
5. 5 in deoxycholate solution. Biochemistry 1975, 14, 1194-1199.
6. 5 in aqueous solution. J. Biol. Chem. 1976, 251, 2113-2118.
7. 5 augments the 17,20-lyase activity of human P450c17 without direct electron transfer. J. Biol. Chem. 1998, 273, 3158-3165.
8. 5. Arch. Biochem. Biophys. 1994, 315, 438-444.
9. Strittmatter, P.; Fleming, P.; Connors, M.; Corcoran, D. In Methods in Enzymology; Fleischer, S., Packer, L., Eds.; Academic Press: New York, 1978; Vol. 52, 97-101.
10. Bellstedt, D.U.; Human, P.A.; Rowland, G.F.; van der Merwe, K.J. Acid-treated, naked bacteria as immune carriers for protein antigens. Immunol. Methods 1987, 249-255.
11. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 15, 680-685.
12. Doebler, R.; Basaran, N.; Goldston, H.; Holloway, P.W. Effect of protein aggregation in the aqueous phase on the binding of membrane proteins to membranes. Biophys. J. 1999, 76, 928-936.