Mammalian expression vectors for epitope tag fusion proteins that are toxic in E. coli

BioTechniques

Volumn 33, Issue 6, 2002, Pages 1218-1222

  Jakobi, Rolf ^a   McCarthy, Corine C ^a   Koeppel, Mark A ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CLONING; CONSENSUS SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; TATA BOX;

ESCHERICHIA COLI; MAMMALIA; VECTORS;

EID: 0036913212     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02336bm07     Document Type: Article

References (15)

1. new cloning vector and expression strategy for genes encoding proteins toxic to Escherichia coli. Gene 127:99-103.
2. Chalfie, M., Y. Tu, G. Euskirchen, W.W. Ward, and D.C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
3. Evan, G.I., G.K. Lewis, G. Ramsay, and J.M. Bishop. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell Biol. 5:3610-3616.
4. Field, J., J. Nikawa, D. Broek, B. MacDonald, L. Rodgers, I.A. Wilson, R.A. Lerner, and M. Wigler. 1988. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol. Cell Biol. 8:2159-2165.
5. Heim, R., A.B. Cubitt, and R.Y. Tsien. 1995. Improved green fluorescence. Nature 373:663-664.
6. Hopp, T.P., K.S. Pricket, V.L. Price, R.T. Libby, C.J. March, D.P. Cerretti, D.L. Urdal, and P.J. Conlon. 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6:1204-1210.
7. Inouye, S. and F.I. Tsuji. 1994. Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 341:277-280.
8. Jakobi, R., C.J. Chen, P.T. Tuazon, and J.A. Traugh. 1996. Molecular cloning and sequencing of the cytostatic G protein-activated protein kinase PAK I. J. Biol. Chem. 271:6206-6211.
9. Jakobi, R., E. Moertl, and M.A. Koeppel. 2001. p21-activated protein kinase γ-PAK suppresses programmed cell death of BALB3T3 fibroblasts. J. Biol. Chem. 276:16624-16634.
10. Jakobi, R., E. Moertl, and M.A. Koeppel. 2001. Vectors for the generation of FLAG- or EGFP-tagged cDNA constructs and EGFP-tagged antisense RNA constructs. BioTechniques 30:476-482.
11. Kolodziej, P.A. and R.A. Young. 1991. Epitope tagging and protein surveillance. Methods Enzymol. 194:508-519.
12. Kozak, M. 1987. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15:8125-8148.
13. Machesky, L.M. and K.L. Gould. 1999. The Arp2/3 complex: A multifunctional actin organizer. Curr. Opin. Cell Biol. 11:117-121.
14. Prasher, D.C., V.K. Eckenrode, W.W. Ward, F.G. Prendergast, and M.J. Cormier. 1992. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229-233.
15. Welch, M.D. 1999. The world according to Arp: Regulation of actin nucleation by the Arp2/3 complex. Trends Cell Biol. 9:423-427.