Signature Tagged Mutagenesis of Haemophilus influenzae identifies genes required for in vivo survival

Microbial Pathogenesis

Volumn 33, Issue 5, 2002, Pages 211-223

  Herbert, M A ^a   Hayes, S ^a   Deadman, M E ^a   Tang, C M ^a   Hood, D W ^a   Moxon, E R ^a  

^a JOHN RADCLIFFE HOSPITAL   (United Kingdom)

Author keywords

Haemophilus influenzae; Pathogenesis; Signature Tagged Mutagenesis

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL SURVIVAL; BACTERIAL VIRULENCE; CONTROLLED STUDY; GENE MUTATION; GENOME ANALYSIS; HAEMOPHILUS INFLUENZAE; IN VIVO STUDY; INFLUENZA; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN MOTIF; SEQUENCE HOMOLOGY; TRANSPOSON;

ANIMALIA; BACTERIA (MICROORGANISMS); HAEMOPHILUS; HAEMOPHILUS INFLUENZAE; TRANSPOSONS;

EID: 0036910804     PISSN: 08824010     EISSN: None     Source Type: Journal    
DOI: 10.1006/mpat.2002.0530     Document Type: Article

References (37)

1. Moxon ER. Haemophilus influenzae. In: Mandell GL, Bennett JE and Dolin, R, Eds. Principles and Practice of Infectious Diseases. New York: Churchill Livingstone, 1991: 2039-44.
2. Jordens JZ, Slack MP. Haemophilus influenzae: Then and now. Eur J Clin Microbiol Infect Dis 1995; 14: 935-48.
3. Murphy TF, Apicella MA. Nontypable Haemophilus influenzae: A review of clinical aspects, surface antigens, and the human immune response to infection. Rev Infect Dis 1987; 9: 1-15.
4. Moxon ER, Wilson R. The role of Haemophilus influenzae in the pathogenesis of pneumonia. Rev Infect Dis 1991; 13 Suppl 6: S518-27.
5. Selwyn BJ. The epidemiology of acute respiratory tract infection in young children: Comparison of findings from several developing countries. Coordinated Data Group of BOSTID Researchers. Rev Infect Dis 1990; 12: s870-88.
6. Hensel M, Holden DW. Molecular genetic approaches for the study of virulence in both pathogenic bacteria and fungi. Microbiology 1996; 142: 1049-58.
7. Hensel M, Shea JE, Gleeson C, Jones MD, Dalton E, Holden DW. Simultaneous identification of bacterial virulence genes by negative selection. Science 1995; 269(5222): 400-3.
8. Mecsas J. Use of signature-tagged mutagenesis in pathogenesis studies. Current Opinion Microbiol 2002; 5: 33-7.
9. Stevens MK, Cope LD, Radolf JD, Hansen EJ. A system for generalized mutagenesis of Haemophilus ducreyi. Infect Immun 1995; 63: 2976-82.
10. Nassif X, Puaoi D, So M. Transposition of Tn1545-Δ3 in the pathogenic Neisseriae: A genetic tool for mutagenesis. J Bacteriol 1991; 173: 2147-54.
11. Smith AL, Smith DH, Averill DM, Marino J, Moxon ER. Production of Haemophilus influenzae b meningitis in infant rats by intraperitoneal inoculation. Infect Immun 1973; 8: 278-90.
12. Moxon ER. Experimental studies of Haemophilus influenzae infection in a rat model. In: Sell SH, Wright PF, Eds. Haemophilus Influenzae. New York: Elsevier Science Publishing Co., 1982: 59-71.
13. Martindale J, Stroud D, Moxon ER, Tang CM. Genetic analysis of Escherichia coli K1 gastrointestinal colonization. Mol Microbiol 2000; 37: 1293-305.
14. Apweiler R, Attwood TK, Bairoch A et al. The InterPro database, an integrated documentation resource for protein families, domains and functional sites. Nucl Acids Res 2001; 29: 37-40.
15. Lomovskaya O, Lewis K, Matin A. EmrR is a negative regulator of Escherichia coli multidrug resistance pump EmrAB. J Bacteriol 1995; 177: 2328-34.
16. Weiser JN, Williams A, Moxon ER. Phase-variable lipopolysaccharide structures enhance the invasive capacity of Haemophilus influenzae. Infect Immun 1990; 58: 3455-7.
17. St. Geme JW III, Cutter D. The Haemophilus influenzae Hia adhesin is an autotransporter protein that remains uncleaved at the C terminus and fully cell associated. J Bacteriol 2000; 182: 6005-18.
18. Scott JR, Churchward GG. Conjugative transposition. Annu Rev Microbiol 1995; 49: 367-97.
19. Trieu-Cuot P, Poyart-Salmeron C, Carlier C, Courvalin P. Sequence requirements for target activity in site-specific recombination mediated by the Int protein of transposon Tn 1545. Mol Microbiol 1993; 8: 179-85.
20. Macfadyen LP, Redfield RJ. Life in mucus: Sugar metabolism in Haemophilus influenzae. Res Microbiol 1996; 147: 541-51.
21. Kuratana M, Inzana TJ, Anderson P. Sources of low-molecular-weight host factors that phenotypically increase the resistance of Haemophilus influenzae type b to bacteriolysis: Non-identity with a factor active in gonococci. Microb Pathog 1989; 7: 73-7.
22. Fleischmann RD, Adams MD, White O et al. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 1995; 269(5223): 496-512.
23. Kroll JS, Loynds BM, Moxon ER. The Haemophilus influenzae capsulation gene cluster: A compound transposon. Mol Microbiol 1991; 5: 1549-60.
24. Kroll JS, Moxon ER, Loynds BM. An ancestral mutation enhancing the fitness and increasing the virulence of Haemophilus influenzae type b. J Inf Dis 1993; 168: 172-6.
25. Sun YH, Bakshi S, Chalmers R, Tang CM. Functional genomics of Neisseria meningitidis pathogenesis. Nat Med 2000; 6: 1269-73.
26. Akerley BJ, Rubin EJ, Camilli A, Lampe DJ, Robertson HM, Mekalanos JJ. Systematic identification of essential genes by in vitro Mariner mutagenesis. Proc Natl Acad Sci USA 1998; 95: 8927-32.
27. Butler PD, Moxon ER. A physical map of the genome of Haemophilus influenzae type b. J Gen Microbiol 1990; 136(Pt 12): 2333-42.
28. Zwahlen A, Rubin LG, Moxon, ER. Contribution of lipopolysaccharide to pathogenicity of Haemophilus influenzae: Comparative virulence of genetically related strains in rats. Microb Pathog 1986; 1: 465-73.
29. Wilcox KW, Smith, HO. Isolation and characterization of mutants of Haemophilus influenzae deficient in an adenosine 5′-triphosphate-dependent deoxyribonu-clease activity. J Bacteriol 1975; 122: 443-53.
30. Barcak GJ, Chandler MS, Redfield RJ, Tomb J-F. Genetic systems in Haemophilus influenzae. Methods Enzymol 1991; 204: 321-42.
31. Simon R, Priefer U, Puhler A. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagensis in gram negative bacteria. BioTechnology 1983; 8: 784-91.
32. Palmer BR, Marinus MG. The dam and dcm strains of Escherichia coli - A review. Gene 1994; 143: 1-12.
33. Ausubel FM, Brent R, Kingston RE, Moore DD. Current Protocols in Molecular Cloning. New York: Wiley, 1989.
34. Wilson K. Preparation of genomic DNA from bacteria. Current Protocols in Molecular Biology 1987; 2.4.1-2.4.5.
35. Mei JM, Nourbakhsh F, Ford CW, Holden DW. Identification of Staphylococcus aureus virulence genes in a murine model of bacteraemia using signature-tagged mutagenesis. Mol Microbiol 1997; 26: 399-407.
36. Totten PA, Norn DV, Stamm WE. Characterization of the hemolytic activity of Haemophilus ducreyi. Infect Immun 1995; 63: 4409-16.
37. Hosking SL, Deadman ME, Moxon ER, Peden JF, Saunders NJ, High NJ. An in silico evaluation of Tn916 as a tool for generalized mutagenesis in Haemophilusinfluenzae Rd. Microbiology 1998; 144: 2525-30.