A microassay for protein determination using microwaves

Analytical Biochemistry

Volumn 311, Issue 1, 2002, Pages 84-86

  Jain, Sulakshana ^a   Sharma, Shweta ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

MICROASSAY; PROTEIN DETERMINATION;

CHEMISTRY;

AMMONIUM SULFATE; BOVINE SERUM ALBUMIN; CHYMOTRYPSIN A; CITRIC ACID; EDETIC ACID; GLYCINE; IMMUNOGLOBULIN G; LYSOZYME; MERCAPTOETHANOL; PHOSPHATE; PROTEIN; REAGENT; RIBONUCLEASE; SOYBEAN TRYPSIN INHIBITOR; TRITON X 100; TRYPSIN; UREA;

ANALYTIC METHOD; ARTICLE; ASSAY; CHEMICAL ANALYSIS; COLOR; CONCENTRATION RESPONSE; MICROWAVE RADIATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; ANIMAL; CHEMISTRY; METHODOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY; STANDARD;

BOVINAE; GLYCINE MAX;

ANIMALS; MICROWAVES; PROTEINS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SERUM ALBUMIN, BOVINE; SPECTROPHOTOMETRY;

EID: 0036903608     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00378-0     Document Type: Article

References (12)

1. R.E. Akins, R.S. Tuan, in: J.M. Walker (Ed.), The Proteins Protocol Handbook, Humana Press, Clifton, NJ, 1996, pp. 21-28.
2. O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the phenol reagent, J. Biol. Chem. 193 (1951) 265-275.
3. J.D.-E. Young, C.-C. Liu, L.G. Leong, A. Damiano, P.M. Persechini, Z.A. Chn, An automated colorimetric microassay system for rapid and quantitative determination of soluble enzymatic activities, Biotechniques 5 (1987) 574-577.
4. G.L. Peterson, A simplification of the protein assay method of Lowry et al which is more generally applicable, Anal. Biochem. 83 (1977) 346-356.
5. H.P.S. Makkar, O.P. Sharma, Negi S.S, Assay of proteins by Lowry's method in the presence of β-mercaptoethanol, Anal. Biochem. 104 (1980) 124-126.
6. M.C. Stocheck, in: M.P. Deutscher (Ed.), Methods in Enzymology, vol. 182, Academic press, San Deigo, 1990, pp. 50-68.
7. D.A. Lewis, J.D. Summers, T.C. Ward, J.E. Mcgrath, Accelerated imidization reactions using microwave radiation, J. Polym. Sci. 30 (1992) 1647-1653.
8. G. Bram, A. Loupy, M. Majdoub, E. Gutierrez, E. Ruiz-Hitzky, Alkylation of potassium acetate in "dry media" thermal activation in commercial microwave ovens, Tetrahedron 46 (1990) 5167-5176.
9. M.-C. Parker, T. Besson, S. Lamare, M.-D. Legoy, Microwave radiation can increase the rate of enzyme-catalysed reactions in organic media, Tetrahedron Lett. 37 (1996) 8383-8386.
10. M. Kidwai, P. Misra, K.R. Bhushan, Microwave assisted synthesis of novel organomercurials in 'dry media', Polyhedron 18 (1999) 2641-2643.
11. S. Caddick, Microwave assisted organic reactions, Tetrahedron 51 (1995) 10403-10432.
12. M.N. Gupta, in: M.N. Gupta (Ed.), Methods in Non-aqueous Enzymology, Birkhäuser, Basel, 2000, pp. 1-13.