Construction of engineered water-soluble PQQ glucose dehydrogenase with improved substrate specificity

Biocatalysis and Biotransformation

Volumn 20, Issue 6, 2002, Pages 405-412

  Sode, Koji ^a   Igarashi, Satoshi ^a   Morimoto, Akifumi ^a   Yoshida, Hiromi ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Propeller protein; Glucose dehydrogenase; Glucose sensor; Protein engineering; Pyrroloquinolone quinone (PQQ); Substrate specificity

Indexed keywords

AMINO ACIDS; CATALYSIS; GLUCOSE;

MUTANTS;

ENZYMES;

3 METHYLGLUCOSE; ALLOSE; AMINO ACID; ASPARAGINE; EDETIC ACID; GALACTOSE; GLUCOSE DEHYDROGENASE; HISTAMINE; ISOLEUCINE; LACTOSE; LYSINE; MALTOSE; PYRROLOQUINOLINEQUINONE; THREONINE; WATER;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; CATALYSIS; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SPECIFICITY; ENZYME SUBSTRATE; MUTANT; NONHUMAN; SITE DIRECTED MUTAGENESIS; SOLUBILITY; STRUCTURAL HOMOLOGY; THERMOSTABILITY; WILD TYPE;

EID: 0036901886     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.1080/1024242021000058694     Document Type: Article

References (26)

1. Cleton-Jansen, A.-M., Goosen, N., Fayet, O. and Van de Putte, P. (1990) "Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase", J. Bacteriol. 172, 6308-6315.
2. D'Costa, E.J., Higgins, I.J. and Turner, A.P.F. (1986) "Quinoprotein glucose dehydrogenase and its application in an amperometric glucose sensor", Biosensor 2, 71-87.
3. 2+-assisted, direct hydride transfer, and rate-determining tautomerization of C5-reduced PQQ to PQQH2, in the oxidation of beta-D-glucose by soluble quinoprotein glucose dehydrogenase", Biochemistry 39, 9384-9392.
4. Igarashi, S., Ohtera, T., Yoshida, H., Witarto, A.B. and Sode, K. (1999) "Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered Km values-site-directed mutagenesis studies on the putative active site", Biochem. Biophys. Res. Commun. 264, 820-824.
5. Katz, E., Schlereth, D.D. and Schmidt, H.-L. (1996) "Reconstitution of the quinoprotein glucose dehydrogenase from its apoenzyme on a gold electrode surface modified with a monolayer of pyrroloquinoline quinone", Electroanal. Chem. 368, 165-171.
6. Kost, G.J., Vu, H.-T., Lee, J.H., Bourgeois, P., Kiechle, F.L., Martin, C., Miller, S.S., Okorodudu, A.O., Podczasy, J.J., Webster, R. and Whitlow, K.J. (1998) "Multi-center study of oxygen-insensitive handheld glucose point-of-care testing in critical care/hospital/ambulatory patients in the United States and Canada", Crit. Care Med. 26, 581-590.
7. Olsthoorn, A.J.J. and Duine, J.A. (1998) "On the mechanism and specificity of soluble, quinoprotein glucose dehydrogenase in the oxidation of aldose sugars", Biochemistry 37, 13854-13861.
8. Oubrie, A. and Dijkstra, B.W. (2000) "Structural requirements of pyrroloquinoline quinone dependent enzymatic reactions", Protein Sci. 9, 1265-1273.
9. Oubrie, A., Rozeboom, H.J., Kalk, K.H., Duine, J.A. and Dikstra, B.W. (1999a) "The 1.7 Å crystal structure of the apo form of the soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus reveals a novel internal conserved sequence repeat", J. Mol. Biol. 289, 319-333.
10. Oubrie, A., Rozeboom, H.J., Kalk, K.H., Olsthoorn, A.J.J., Duine, J.A. and Dijkstra, B.W. (1999b) "Structure and mechanism of soluble quinoprotein glucose dehydrogenase", EMBO J. 18, 5187-5194.
11. Oubrie, A., Rozeboom, H.J. and Dijkstra, B.W. (1999c) "Active-site structure of the soluble quinoprotein glucose dehydrogenase complexed with methylhydrazine: A covalent cofactor-inhibitor complex", Proc. Natl. Acad. Sci. USA 96, 11787-11791.
12. Schmidt, B. (1997) "Oxygen-independent oxidases. A new class of enzymes for application in diagnostics", Clin. Chim. Acta 266, 33-37.
13. Sode, K. and Sano, H. (1994) "Glu742 substitution to Lys enhances the EDTA tolerance of Escherichia coli PQQ glucose dehydrogenase", Biotechnol. Lett. 16, 455-460.
14. Sode, K. and Kojima, K. (1997) "Improved substrate specificity and dynamic range for glucose measurement of Escherichia coli PQQ glucose dehydrogenase by site directed mutagenesis", Biotechnol. Lett. 19, 1073-1077.
15. Sode, K. and Yoshida, H. (1997) "Construction and characterization of a chimeric Escherichia coli PQQ glucose dehydrogenase (PQQGDH) with increased EDTA tolerance", Denki Kagaku 65, 444-451.
16. Sode, K., Nakasono, S., Tanaka, M. and Matsunaga, T. (1993) "Subzero temperature operating biosensor utilizing an organic solvent and quinoprotein glucose dehydrogenase", Biotechnol. Bioeng. 42, 251-254.
17. Sode, K., Yoshida, H., Matsumura, K., Kikuchi, T., Watanabe, M., Yasutake, N., Ito, S. and Sano, H. (1995) "Elucidation of the region responsible for EDTA tolerance in PQQ glucose dehydrogenase", Biochem. Biophys. Res. Commun. 211, 268-273.
18. Sode, K., Watanabe, K., Ito, S., Matsumura, K. and Kikuchi, T. (1995b) "Thermostable chimeric PQQ glucose dehydrogenase", FEBS Lett. 364, 325-327.
19. Sode, K., Ohtera, T., Shirahane, M., Witarto, A.B., Igarashi, S. and Yoshida, H. (2000) "Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement", Enz. Microbiol. Technol. 26, 491-496.
20. Yamazaki, T., Kojima, K. and Sode, K. (2000) "Extend-range glucose sensor employing engineered glucose dehydrogenase", Anal. Chem. 72, 4689-4693.
21. Ye, L., Hammerle, M., Olsthoorn, A.J.J., Schuhmann, W., Schmidt, H.-L., Duine, J.A. and Heller, A. (1993) "High current density "wired" quinoprotein glucose dehydrogenase electrode", Anal. Chem. 65, 238-241.
22. Yokoyama, K., Sode, K., Tamiya, E. and Karube, I. (1989) "Integrated biosensor for glucose and galactose", Anal. Chim. Acta 218, 137-142.
23. Yoshida, H. and Sode, K. (1997) "Thr424 to Asn substitution alters bivalent metal specificity of pyrroloquinoline quinone glucose dehydrogenase", J. Biochem. Mol. Biol. Biophys. 1, 89-93.
24. Yoshida, H., Kojima, K., Witarto, A.B. and Sode, K. (1999) "Engineering a chimeric pyrroloquinoline quinone glucose dehydrogenase: Improvement of EDTA tolerance, thermal stability and substrate specificity", Protein Eng. 12, 63-70.
25. Witarto, A.B., Ohuchi, S., Narita, M. and Sode, K. (1999a) "Secondary structure study of pyrroloquinoline quinone glucose dehydrogenase", J. Biochem. Mol. Biol. Biophys. 2, 209-213.
26. Witarto, A.B., Ohtera, T. and Sode, K. (1999b) "Site-directed mutagenesis study on the thermal stability of a chimeric PQQ glucose dehydrogenase and its structural interpretation", Appl. Biochem. Biotechnol. 77-79, 159-168.