Direct and rapid identification and genogrouping of meningococci and porA amplification by LightCycler PCR

Journal of Clinical Microbiology

Volumn 40, Issue 12, 2002, Pages 4531-4535

  Mölling, Paula ^a   Jacobsson, Susanne ^a   Bäckman, Anders ^a   Olcén, Per ^a  

^a ÖREBRO UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANALYTICAL PARAMETERS; ARTICLE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA DETERMINATION; DNA SEQUENCE; GENE AMPLIFICATION; GENOTYPE; MELTING POINT; NEISSERIA MENINGITIDIS; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; TIME;

BACTERIAL TYPING TECHNIQUES; FLUORESCENT DYES; GENOTYPE; HUMANS; MENINGITIS, MENINGOCOCCAL; MENINGOCOCCAL INFECTIONS; NEISSERIA MENINGITIDIS; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; PORINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TEMPERATURE; TIME FACTORS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; NEISSERIA MENINGITIDIS;

EID: 0036898060     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.12.4531-4535.2002     Document Type: Article

References (29)

1. Arbin, F. F., F. Moreau, J. W. Coulton, and E. L. Mills. 1997. Subtyping of Neisseria meningitidis strains isolated in Quebec, Canada: Correlation between deduced amino acid sequences and serosubtyping techniques. Can. J. Microbiol. 43:234-238.
2. Bäckman, A., P. Lantz, P. Rådstrom, and P. Olcén. 1999. Evaluation of an extended diagnostic PCR assay for detection and verification of the common causes of bacterial meningitis in CSF and other biological samples. Mol. Cell. Probes 13:49-60.
3. Bhattacharjee, A. K., H. J. Jennings, C. P. Kenny, A. Martin, and I. C. Smith. 1975. Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. J. Biol. Chem. 250,1926-1932.
4. Bhattacharjee, A. K., H. J. Jennings, C. P. Kenny, A. Martin, and I. C. Smith. 1976. Structural determination of the polysaccharide antigens of Neisseria meningitidis serogroups Y, W-135, and BO1. Can. J. Biochem. 54:1-8.
5. Borrow, R., H. Claus, U. Chaudhry, M. Guíver, E. B. Kaczmarski, M. Frosch, and A. J. Fox. 1998. siaD PCR ELISA for confirmation and identification of serogroup Y and W135 meningococcal infections. FEMS Microbiol. Lett. 159:209-214.
6. Borrow, R., H. Claus, M. Guiver, L. Smart, D. M. Jones, E. B. Kaczmarski, M. Frosch, and A. J. Fox. 1997. Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA. Epidemiol. Infect. 118:111-117.
7. Corless, C. E., M. Guiver, R. Borrow, V. Edwards-Jones, A. J. Fox, and E. B. Kaczmarski. 2001. Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J. Clin. Microbiol. 39:1553-1558.
8. Feavers, I. M., A. J. Fox, S. Gray, D. M. Jones, and M. C. Maiden. 1996. Antigenic diversity of meningococcal outer membrane protein PorA has implications for epidemiological analysis and vaccine design. Clin. Diagn. Lab. Immunol. 3:444-450.
9. Frasch, C. E., W. D. Zollinger, and J. T. Poolman. 1985. Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev. Infect. Dis. 7:504-510.
10. Frosch, M., D. Muller, K. Bousset, and A. Muller. 1992. Conserved outer membrane protein of Neisseria meningitidis involved in capsule expression. Infect. Immun. 60:798-803.
11. Glode, M. P., J. B. Robbins, T. Y. Liu, E. C. Gotschlich, I. Orskov, and F. Orskov. 1977. Cross-antigenicity and immunogenicity between capsular polysaccharides of group C Neisseria meningitidis and of Escherichia coli K92. J. Infect. Dis. 135:94-104.
12. Guirguis, N., R. Schneerson, A. Bax, W. Egan, J. B. Robbins, J. Shiloach, I. Orskov, F. Orskov, and A. el Kholy. 1985. Escherichia coli K51 and K93 capsular polysaccharides are crossreactive with the group A capsular polysaccharide of Neisseria meningitidis. Immunochemical, biological, and epidemiological studies. J. Exp. Med. 162:1837-1851.
13. Guiver, M., R. Borrow, J. Marsh, S. J. Gray, E. B. Kaczmarski, D. Howells, P. Boseley, and A. J. Fox. 2000. Evaluation of the Applied Biosystems automated Taqman polymerase chain reaction system for the detection of meningococcal DNA. FEMS Immunol. Med. Microbiol. 28:173-179.
14. Kasper, D. L., J. L. Winkelhake, W. D. Zollinger, B. L. Brandt, and M. S. Artenstein. 1973. Immunochemical similarity between polysaccharide antigens of Escherichia coli 07: K1(L)ŃM and group B Neisseria meningitidis. J. Immunol. 110:262-268.
15. Kotilainen, P., J. Jalava, O. Meurman, O. P. Lehtonen, E. Rintala, O. P. Seppala, E. Eerola, and S. Nikkari. 1998. Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid. J. Clin. Microbiol. 36:2205-2209.
16. Kristiansen, B. E., E. Ask, A. Jenkins, C. Fermer, P. Rådström, and O. Sköld. 1991. Rapid diagnosis of meningococcal meningitis by polymerase chain reaction. Lancet 337:1568-1569.
17. Lansac, N., F. J. Picard, C. Menard, M. Boissinot, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2000. Novel genus-specific PCR-based assays for rapid identification of Neisseria species and Neisseria meningitidis. Eur. J. Clin. Microbiol. Infect. Dis. 19:443-451.
18. Liu, T. Y., E. C. Gotschlich, F. T. Dunne, and E. K. Jonssen. 1971. Studies on the meningococcal polysaccharides. II. Composition and chemical properties of the group B and group C polysaccharide. J. Biol. Chem. 246:4703-4712.
19. McLaughlin, G. L., D. K. Howe, D. R. Biggs, A. R. Smith, P. Ludwinski, B. C. Fox, D. N. Tripathy, C. E. Frasch, J. D. Wenger, R. B. Carey, et al. 1993. Amplification of rDNA loci to detect and type Neisseria meningitidis and other eubacteria. Mol. Cell. Probes 7:7-17.
20. Mölling, P., M. Unemo, A. Bäckman, and P. Olcén. 2000. Genosubtyping by sequencing group A, B and C meningococci; a tool for epidemiological studies of epidemics, clusters and sporadic cases. APMIS 108:509-516.
21. Olcén, P., D. Danielsson, and J. Kjellander. 1975. The use of protein A containing staphylococci sensitized with anti-meningococcal antibodies for grouping Neisseria meningitidis and demonstration of meningococcal antigen in cerebrospinal fluid. Acta Pathol. Microbiol. Scand. 83:387-396.
22. Orvelid, P., A. Bäckman, and P. Olcén, 1999. PCR identification of the group A Neisseria meningitidis gene in cerebrospinal fluid. Scand. J. Infect. Dis. 31:481-483.
23. Peltola, H. 1983. Meningococcal disease: Still with us. Rev. Infect. Dis. 5:71-91.
24. Porritt, R. J., J. L. Mercer, and R. Munro. 2000. Detection and serogroup determination of Neisseria meningitidis in CSF by polymerase chain reaction (PCR). Pathology 32:42-45.
25. Rådström, P., A, Bäckman, N. Qian, P. Kragsbjerg, C. Påhlson, and P. Olcén. 1994. Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy. J. Clin. Microbiol. 32:2738-2744.
26. Swaminathan, B., G. M. Matar, M. W. Reeves, L. M. Graves, G. Ajello, W. F. Bibb, L. O. Helsel, M. Morales, H. Dronavalli, M. el-Swify, W. DeWitt, and S. B. Hunter. 1996. Molecular subtyping of Neisseria meningitidis serogroup B: Comparison of five methods. J. Clin. Microbiol. 34:1468-1473.
27. Swartley, J. S., L.-J. Liu, Y. K. Miller, L. E. Martin, S. Edupuganti, and D. S. Stephens. 1998. Characterization of the gene cassette required for biosynthesis of the (α1→6)-linked N-acetyl-D-mannosamine-1 -phosphate capsule of serogroup A Neisseria meningitidis. J. Bacteriol. 180:1533-1539.
28. Taha, M. K. 2000. Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis. J. Clin. Microbiol. 38:855-857.
29. Vann, W. F., T. Y. Liu, and J. B. Robbins. 1976. Bacillus pumilus polysaccharide cross-reactive with meningococcal group A polysaccharide. Infect. Immun. 13:1654-1662.