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van der Vlag J., Otte A.P. Transcriptional repression mediated by the human Polycomb-group protein EED involves histone deacetylation. Nat Genet. 23:1999;474-478.
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Van der Vlag, J.1
Otte, A.P.2
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45
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0035141264
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The Drosophila Polycomb group proteins ESC and E(Z) are present in a complex containing the histone-binding protein p55 and the histone deacetylase RPD3
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Purification of the E(Z)-ESC complex from Drosophila revealed the association of both proteins with the histone-binding protein p55 and the histone deacetylase RPD3. The presence of RPD3 is required for repression mediated by a Polycomb-response element in vivo, suggesting that histone deacetylation is a prerequisite for establishment of long-term repression by other continuously required PcG complexes.
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Tie F., Furuyama T., Prasad-Sinha J., Jane E., Harte P.J. The Drosophila Polycomb group proteins ESC and E(Z) are present in a complex containing the histone-binding protein p55 and the histone deacetylase RPD3. Development. 128:2001;275-286. Purification of the E(Z)-ESC complex from Drosophila revealed the association of both proteins with the histone-binding protein p55 and the histone deacetylase RPD3. The presence of RPD3 is required for repression mediated by a Polycomb-response element in vivo, suggesting that histone deacetylation is a prerequisite for establishment of long-term repression by other continuously required PcG complexes.
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(2001)
Development
, vol.128
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Tie, F.1
Furuyama, T.2
Prasad-Sinha, J.3
Jane, E.4
Harte, P.J.5
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46
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0035793060
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Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development
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Tian L., Chen Z.J. Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development. Proc Natl Acad Sci USA. 98:2001;200-205.
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Proc Natl Acad Sci USA
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Tian, L.1
Chen, Z.J.2
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47
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0033538578
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Stabilization of chromatin structure by PRC1, a Polycomb complex
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Shao Z., Raible F., Mollaaghababa R., Guyon J.R., Wu C.T., Bender W., Kingston R.E. Stabilization of chromatin structure by PRC1, a Polycomb complex. Cell. 98:1999;37-46.
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Cell
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Shao, Z.1
Raible, F.2
Mollaaghababa, R.3
Guyon, J.R.4
Wu, C.T.5
Bender, W.6
Kingston, R.E.7
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48
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0035476530
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Establishment of Polycomb silencing requires a transient interaction between PC and ESC
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Two distinct PcG complexes have been identified in Drosophila and vertebrates, one containing E(Z) and ESC, and the Polycomb (PC)-containing complex PRC1. Using LexA fusions, it was shown that ESC and PC can mediate repression but that each requires the presence of the other. PC was found both in the early-acting complex containing ESC and E(Z), and in the later-acting PRC1 complex. This suggests that PC mediates the interaction between early- and late-acting PcG complexes.
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Poux S., Melfi R., Pirrotta V. Establishment of Polycomb silencing requires a transient interaction between PC and ESC. Genes Dev. 15:2001;2509-2514. Two distinct PcG complexes have been identified in Drosophila and vertebrates, one containing E(Z) and ESC, and the Polycomb (PC)-containing complex PRC1. Using LexA fusions, it was shown that ESC and PC can mediate repression but that each requires the presence of the other. PC was found both in the early-acting complex containing ESC and E(Z), and in the later-acting PRC1 complex. This suggests that PC mediates the interaction between early- and late-acting PcG complexes.
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(2001)
Genes Dev
, vol.15
, pp. 2509-2514
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Poux, S.1
Melfi, R.2
Pirrotta, V.3
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49
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0036010498
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Sequence relationships, conserved domains, and expression patterns for maize homologs of the Polycomb group genes E(z), esc, and E(Pc)
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Springer N.M., Danilevskaya O.N., Hermon P., Helentjaris T.G., Phillips R.L., Kaeppler H., Kaeppler S.M. Sequence relationships, conserved domains, and expression patterns for maize homologs of the Polycomb group genes E(z), esc, and E(Pc). Plant Physiol. 128:2002;1332-1345.
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(2002)
Plant Physiol
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Springer, N.M.1
Danilevskaya, O.N.2
Hermon, P.3
Helentjaris, T.G.4
Phillips, R.L.5
Kaeppler, H.6
Kaeppler, S.M.7
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50
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Drosophila Enhancer of zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites
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published online September 13. DOI 10.1016/S0092867402009753
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Czermin B, Melfi R, McCabe D, Seitz V, Imhof A, Pirrotta V: Drosophila Enhancer of zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites. Cell 2002, published online September 13. DOI 10.1016/S0092867402009753.The E(Z)-ESC complex from Drosophila was shown to contain a histone methyltransferase activity that methylates Lys9 and Lys27 of histone H3. This activity is lost when the SET domain of E(Z) is mutated.
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(2002)
Cell
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Czermin, B.1
Melfi, R.2
McCabe, D.3
Seitz, V.4
Imhof, A.5
Pirrotta, V.6
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51
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18644383738
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Histone methyltransferase activity of a Drosophila Polycomb group repressor complex
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published online September 13. DOI 10.1016/S0092867402009765
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Müller J, Hart CM, Francis NJ, Vargas ML, Sengupta A, Wild B, Miller EL, O'Connor MB, Kingston RE, Simon JA: Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell 2002, published online September 13. DOI 10.1016/S0092867402009765.The E(Z)-ESC complex from Drosophila was bichemically purified and it was shown that the SU(Z)12 and NURF-55 proteins are subunits of this complex. Furthermore it was demonstrated that this complex has histone methyltransferase activity for Lys27 of histone H3.
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(2002)
Cell
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Müller, J.1
Hart, C.M.2
Francis, N.J.3
Vargas, M.L.4
Sengupta, A.5
Wild, B.6
Miller, E.L.7
O'Connor, M.B.8
Kingston, R.E.9
Simon, J.A.10
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52
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0036830642
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Role of histone H3 lysine 27 methylation in Polycomb group silencing
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published online September 26
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Cao R, Wang L, Wang H, Xia L, Erdjument-Bromage H, Tempst P, Jones RS, Zhang Y: Role of histone H3 lysine 27 methylation in Polycomb group silencing. Science 2002, published online September 26.The EZH2-EED complex from human was biochemically purified and was shown to have histone methyltransferase activity with specificity for Lys27 of histone H3. This methylation mark is dependent on binding of E(Z) to a Ultrabithorax (Ubx) Polycomb response element and correlates with Ubx repression.
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(2002)
Science
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Cao, R.1
Wang, L.2
Wang, H.3
Xia, L.4
Erdjument-Bromage, H.5
Tempst, P.6
Jones, R.S.7
Zhang, Y.8
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