The effect of mutations on the structure of insulin fibrils studied by Fourier transform infrared (FTIR) spectroscopy and electron microscopy

Journal of Pharmaceutical Sciences

Volumn 91, Issue 12, 2002, Pages 2473-2480

  Garriques, Liza Nielsen ^a,b,c,d   Frokjaer, Sven ^a   Carpenter, John F ^c   Brange, Jens ^b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b NOVO NORDISK A S   (Denmark)

^c UNIVERSITY OF COLORADO   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e Brange Consult   (Denmark)

Author keywords

Aggregation; Insulin analogues; Intermolecular sheet; Morphology; Secondary structure

Indexed keywords

BOVINE INSULIN; HUMAN INSULIN; INSULIN; INSULIN DERIVATIVE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CONFERENCE PAPER; DRUG MANUFACTURE; DRUG STRUCTURE; ELECTRON MICROSCOPY; FIBER; FOURIER TRANSFORMATION; HEATING; HORMONE STRUCTURE; INFRARED SPECTROSCOPY; PROTEIN AGGREGATION; PROTEIN SECONDARY STRUCTURE; SEQUENCE ANALYSIS; WILD TYPE; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; GENETICS; HUMAN; METHODOLOGY; MUTATION; PROTEIN TERTIARY STRUCTURE;

ANIMALS; CATTLE; HUMANS; INSULIN; MICROSCOPY, ELECTRON; MUTATION; PROTEIN STRUCTURE, TERTIARY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

EID: 0036892718     PISSN: 00223549     EISSN: None     Source Type: Journal    
DOI: 10.1002/jps.10238     Document Type: Conference Paper

References (19)

1. Manning MC, Patel K, Borchardt RT. 1989. Stability of protein pharmaceuticals. Pharm Res 6:903-918.
2. Brange J, Andersen L, Laursen ED, Meyn G, Rasmussen E. 1997. Toward understanding insulin fibrillation. J Pharm Sci 86:517-525.
3. Fink AL. 1998. Protein aggregation - Folding aggregates, inclusion-bodies and amyloid. Folding Design 3:R9-R23.
4. Uversky VN, Talapatra A, Gillespie JR, Fink AL. 1999. Protein deposits as the molecular basis of amyloidosis. Part I. Systemic amyloidoses. Med Sci Monit 5:1001-1012.
5. Uversky VN, Talapatra A, Gillespie JR, Fink AL. 1999. Protein deposits as the molecular basis of amyloidosis. Part II. Localized amyloidosis and neurodegenerative disorders. Med Sci Monit 5:1238-1254.
6. Brange J, Dodson GG, Edwards DJ, Holden PH, Whittingham JL. 1997. A model of insulin fibrils derived from the X-ray crystal structure of a monomeric insulin (despentapeptide insulin). Proteins: Struct Funct Genet 27:507-516.
7. Brange J, Whittingham J, Edwards D, Youshang Z, Wollmer A, Brandenburg D, Dodson G, Finch J. 1997. Insulin structure and diabetes treatment. Curr Sci 72:470-476.
8. Brange J, Owens DR, Kang S, Volund A. 1990. Monomeric insulins and their experimental and clinical implications. Diabetes Care 13:923-954.
9. Ambrose EJ, Elliot A. 1951. Infrared spectroscopic studies of globular protein structure. Proc R Soc Lond A 208:75-90.
10. Pikal MJ, Rigsbee DR. 1997. The stability of insulin in crystalline and amorphous solids: Observation of greater stability for the amorphous form. Pharm Res 14:1379-1387.
11. Timasheff SN, Susi H, Stevens L. 1967. Infrared spectra and protein conformations in aqueous solutions. J Biol Chem 5467-5473.
12. Nielsen L, Frokjaer S, Carpenter JF, Brange J. 2001. Studies of the structure of insulin fibrils by FTIR-spectroscopy and electron microscopy. J Pharm Sci 90:29-37.
13. Dong AC, Prestrelski SJ, Allison SD, Carpenter JF. 1995. Infrared spectroscopic studies of lyophilization-induced and temperature-induced protein aggregation. J Pharm Sci 84:415-424.
14. Prestrelski SJ, Tedeschi N, Arakawa T, Carpenter JF. 1993. Dehydration-induced conformational transitions in proteins and their inhibition by stabilizers. Biophys J 65:661-671.
15. Kendrick BS, Cleland JL, Lam X, Nguyen T, Randolph TW, Manning MC, Carpenter JF. 1998. Aggregation of recombinant human interferon gamma: Kinetics and structural transitions. J Pharm Sci 87:1069-1076.
16. Nielsen L, Khurana R, Coats A, Frokjaer S, Brange J, Vyas S, Uversky VN, Fink AL. 2001. The effect of environmental factors on the kinetics of insulin fibril formation: Elucidation of the molecular mechanism. Biochemistry 40:6036-6046.
17. Nielsen L, Frokjaer S, Brange J, Uversky VN, Fink AL. 2001. Probing the mechanism of insulin fibril formation with insulin mutants. Biochemistry 40:8397-8409.
18. Dong A, Caughey WS. 1994. Infrared methods for study of hemoglobin reactions and structures. Methods Enzymol 232:139-175.
19. Dunn MF, Pattison SE, Storm MC, Quiel E. 1980. Comparison of the zinc binding domains in the 7S nerve growth factor and the zinc-insulin hexamer. Biochemistry 19:718-725.