Construction of a novel transposon mutagenesis system useful in the isolation of streptococcus parasanguis mutants defective in Fap1 glycosylation

Infection and Immunity

Volumn 70, Issue 12, 2002, Pages 6534-6540

  Chen, Qiang ^a   Wu, Hui ^a   Fives Taylor, Paula M ^a,b  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF VERMONT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; FIMBRIA ASSOCIATED PROTEIN 1; MONOCLONAL ANTIBODY; PROTEIN; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; BACTERIUM ADHERENCE; BACTERIUM MUTANT; CELL SURFACE; DNA LIBRARY; FIMBRIA; GENE ISOLATION; GENE LOCUS; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; SOUTHERN BLOTTING; STREPTOCOCCUS PARASANGUINIS; TRANSPOSON;

DNA TRANSPOSABLE ELEMENTS; FIMBRIAE PROTEINS; FIMBRIAE, BACTERIAL; GLYCOSYLATION; HUMANS; MICROSCOPY, ELECTRON; MUTAGENESIS, INSERTIONAL; MUTATION; STREPTOCOCCUS;

EID: 0036892188     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.70.12.6534-6540.2002     Document Type: Article

References (36)

1. Arora, S. K., M. Bangera, S. Lory, and R. Ramphal, 2001. A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylation. Proc. Natl. Acad. Sci. USA 98:9342-9347.
2. Benz, I., and M. A. Schmidt. 2001. Glycosylation with heptose residues mediated by the aah gene product is essential for adherence of the AIDA-I adhesin. Mol. Microbiol. 40:1403-1413.
3. Boyer, H. W., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-472.
4. Byrne, M. E., D. A. Rouch, and R. A. Skurray. 1989. Nucleotide sequence analysis of IS256 from the Staphylococcus aureus gentamicin-tobramycinkanamycin-resistance transposon Tn4001. Gene 81:361-367.
5. Calabi, E., S. Ward, B. Wren, T. Paxton, M. Panico, H. Morris, A. Dell, G. Dougan, and N. Fairweather. 2001. Molecular characterization of the surface layer proteins from Clostridium difficile. Mol. Microbiol. 40:1187-1199.
6. Cole, R. M., G. B. Calandra, E. Huff, and K. M. Nugent, 1976. Attributes of potential utility in differentiating among "group H" streptococci or Streptococcus sanguis. J. Dent. Res. 55:A142-A153.
7. Curtis, M. A., A. Thickett, J. M. Slaney, M. Rangarajan, J. Aduse-Opoku, P. Shepherd, N. Paramonov, and E. F. Hounsell. 1999. Variable carbohydrate modifications to the catalytic chains of the RgpA and RgpB proteases of Porphyromonas gingivalis W50. Infect. Immun. 67:3816-3823.
8. Dybvig, K., C. T. French, and L. L. Voelker. 2000. Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis. J. Bacteriol. 182:4343-4347.
9. Elder, B. L., D. K. Boraker, and P. M. Fives-Taylor. 1982. Whole-bacterial-cell enzyme-linked immunosorbent assay for Streptococcus sanguis fimbrial antigens. J. Clin. Microbiol. 16:141-144.
10. Elder, B. L., and P. Fives-Taylor. 1986. Characterization of monoclonal antibodies specific for adhesion: Isolation of an adhesin of Streptococcus sanguis FW213. Infect. Immun. 54:421-427.
11. Elder, L. B. 1984. Ph.D. thesis. University of Vermont, Burlington.
12. Fachon-Kalweit, S., B. L. Elder, and P. Fives-Taylor. 1985. Antibodies that bind to fimbriae block adhesion of Streptococcus sanguis to saliva-coated hydroxyapatite. Infect. Immun. 48:617-624.
13. Fenno, J. C., A. Shaikh, and P. Fives-Taylor. 1993. Characterization of allelic replacement in Streptococcus parasanguis: Transformation and homologous recombination in a 'nontransformable' streptococcus. Gene 130:81-90.
14. Fives-Taylor, P. M., and D. W. Thompson. 1985. Surface properties of Streptococcus sanguis FW213 mutants nonadherent to saliva-coated hydroxyapatite. Infect. Immun. 47:752-759.
15. Foissac, X., C. Saillard, and J. M. Bove. 1997. Random insertion of transposon Tn4001 in the genome of Spiroplasma citri strain GII3. Plasmid 37:80-86.
16. Framson, P. E., A. Nittayajarn, J. Merry, P. Youngman, and C. E. Rubens. 1997. New genetic techniques for group B streptococci: High-efficiency transformation, maintenance of temperature-sensitive pWV01 plasmids, and mutagenesis with Tn917. Appl. Environ. Microbiol. 63:3539-3547.
17. Gutierrez, J. A., P. J. Crowley, D. P. Brown, J. D. Hillman, P. Youngman, and A. S. Bleiweis. 1996. Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: Preliminary characterization of mutants displaying acid sensitivity and nutritional requirements. J. Bacteriol. 178:4166-4175.
18. Israelsen, H., and E. B. Hansen. 1993. Insertion of transposon Tn917 derivatives into the Lactococcus lactis chromosome. Appl. Environ. Microbiol. 59:21-26.
19. Lunsford, R. D. 1995. A Tn4001 delivery system for Streptococcus gordonii (Challis). Plasmid 33:153-157.
20. Lyon, B. R., J. W. May, and R. A. Skurray. 1984. Tn4001: A gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Mol. Gen. Genet. 193:554-556.
21. Lyon, W. R., C. M. Gibson, and M. G. Caparon. 1998. A role for trigger factor and an rgg-like regulator in the transcription, secretion and processing of the cysteine proteinase of Streptococcus pyogenes. EMBO J. 17:6263-6275.
22. Macrina, F. L., J. A. Tobian, K. R. Jones, R. P. Evans, and D. B. Clewell. 1982. A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis. Gene 19:345-353.
23. Maguin, E., P. Duwat, T. Hege, D. Ehrlich, and A. Gruss. 1992. New thermosensitive plasmid for gram-positive bacteria. J. Bacteriol. 174:5633-5638.
24. Mahairas, G. G., B. R. Lyon, R. A. Skurray, and P. A. Pattee. 1989. Genetic analysis of Staphylococcus aureus with Tn4001. J. Bacteriol. 171:3968-3972.
25. Moens, S., and J. Vanderleyden. 1997. Glycoproteins in prokaryotes. Arch. Microbiol. 168:169-175.
26. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
27. Schaffer, C., M. Graninger, and P. Messner. 2001. Prokaryotic glycosylation. Proteomics 1:248-261.
28. Schneewind, O., A. Fowler, and K. F. Faull. 1995. Structure of the cell wall anchor of surface proteins in Staphylococcus aureus. Science 268:103-106.
29. Stephenson, A. E. 2001. Ph.D. thesis. University of Vermont, Burlington.
30. Stephenson, A. E., H. Wu, J. Novak, M. Tomana, K. Mintz, and P. Fives-Taylor. 2002. The Fap1 fimbrial adhesin is a glycoprotein: Antibodies specific for the glycan moiety block the adhesion of Streptococcus parasanguis in an in vitro tooth model. Mol. Microbiol. 43:147-157.
31. Szymanski, C. M., R. Yao, C. P. Ewing, T. J. Trust, and P. Guerry. 1999. Evidence for a system of general protein glycosylation in Campylobacter jejuni. Mol. Microbiol. 32:1022-1030.
32. Tomich, P. K., F. Y. An, and D. B. Clewell. 1980. Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis. J. Bacteriol. 141:1366-1374.
33. Weerkamp, A. H., H. C. van der Mei, and R. S. Liem. 1986. Structural properties of fibrillar proteins isolated from the cell surface and cytoplasm of Streptococcus salivarius (K+) cells and nonadhesive mutants. J. Bacteriol. 165:756-762.
34. Wu, H., and P. M. Fives-Taylor. 1999. Identification of dipeptide repeats and a cell wall sorting signal in the fimbriae-associated adhesin, Fap1, of Streptococcus parasanguis. Mol. Microbiol. 34:1070-1081.
35. Wu, H., K. P. Mintz, M. Ladha, and P. M. Fives-Taylor. 1998. Isolation and characterization of Fap1, a fimbriae-associated adhesin of Streptococcus parasanguis FW213. Mol. Microbiol. 28:487-500.
36. Youngman, P. 1987. Plasmid vectors for recovering and exploiting Tn917 transpositions in Bacillus and other gram-positive bacteria, p. 79-103. In K. G. Hardy (ed.), Plasmids: A practical approach. IRL Press, Washington, D.C.