Overexpression of PrPc triggers caspase 3 activation: Potentiation by proteasome inhibitors and blockade by anti-PrP antibodies

Journal of Neurochemistry

Volumn 83, Issue 5, 2002, Pages 1208-1214

  Paitel, E ^a   Alves da Costa, C ^a   Vilette, D ^b   Grassi, J ^c   Checler, F ^a  

^a INSTITUT DE PHARMACOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^b CEMAGREF   (France)

^c CEA SACLAY   (France)

Author keywords

Antibody sequestration; Apoptosis; Caspase 3; HEK293; Prion; Proteasome

Indexed keywords

CASPASE 3; DNA FRAGMENT; DOXYCYCLINE; PRION PROTEIN; PRION PROTEIN ANTIBODY; PROTEASOME INHIBITOR; PROTEIN ANTIBODY; STAUROSPORINE; UNCLASSIFIED DRUG; ANTIBODY; CASP3 PROTEIN, HUMAN; CASP3 PROTEIN, MOUSE; CASPASE; CYSTEINE PROTEINASE; ENZYME INHIBITOR; MULTIENZYME COMPLEX; PROTEASOME;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DEATH; CELL LINE; CELL SURFACE; CYTOTOXICITY; ENZYME ACTIVATION; ENZYME ACTIVITY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SENSITIZATION; ANIMAL; BIOSYNTHESIS; CELL MEMBRANE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; KIDNEY; METABOLISM; MOUSE; SHEEP;

ANIMALIA;

ANIMALS; ANTIBODIES; APOPTOSIS; CASPASE 3; CASPASES; CELL LINE; CELL MEMBRANE; CYSTEINE ENDOPEPTIDASES; DNA FRAGMENTATION; DOXYCYCLINE; ENZYME ACTIVATION; ENZYME INHIBITORS; GENE EXPRESSION REGULATION; HUMANS; KIDNEY; MICE; MULTIENZYME COMPLEXES; PROTEASOME ENDOPEPTIDASE COMPLEX; PRPC PROTEINS; SHEEP; STAUROSPORINE; TRANSFECTION;

EID: 0036882127     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2002.01234.x     Document Type: Article

References (36)

1. Aguzzi A., Montrasio F. and Kaeser P. S. (2001) Prions: Health scare and biological challenge. Nat. Rev. Mol. Cell Biol. 2, 118-126.
2. Alves da Costa C., Ancolio K. and Checler F. (2000) Wild-type but not Parkinson's disease-related Ala53Thr-α-synuclein protect neuronal cells from apoptotic stimuli. J. Biol. Chem. 275, 24065-24069.
3. Bounhar Y., Zhang Y., Goodyer C. G. and LeBlanc A. (2001) Prion protein protects human neurons against Bax-mediated apoptosis. J. Biol. Chem. 276, 39145-39149.
4. Bradford M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Ann. Biochem. 72, 248-259.
5. Brandner S., Isenmann S., Raeber A., Fischer M., Sailer A., Kobayashi Y., Marino S., Weissmann C. and Aguzzi A. (1996) Normal host prion protein necessary for scrapie-induced neurotoxicity. Nature 379, 339-343.
6. Brown D. R. (1999) Prion protein peptide neurotoxicity can be mediated by astrocytes. J. Neurochem. 73, 1105-1113.
7. Büeler H., Fischer M., Lang Y., Bluethmann H., Lipp H., DeArmond S., Prusiner S. B., Aguet M. and Weissmann C. (1992) Normal development and behaviour of mice lacking the neuronal cellsurface PrP protein. Nature 356, 577-582.
8. Büeler H., Aguzzi A., Sailer A., Greiner R., Autenried P., Aguet M. and Weissmann C. (1993) Mice devoid of PrP are resistant to scrapie. Cell 73, 1339-1347.
9. Checler F. (1995) Processing of the β-amyloid precursor protein and its regulation in Alzheimer's disease. J. Neurochem. 65, 1431-1444.
10. Chen S. G., Teplow D. B., Parchi P., Teller J. K., Gambetti P. and Autilio-Gambetti L. (1995) Truncated forms of the human prion protein in normal brain and in prion disease. J. Biol. Chem. 270, 19173-19180.
11. Chiarini L. B., Freitas A. R. O., Zanata S. M., Brentani R. R., Martins V. R. and Linden R. (2002) Cellular prion protein transduces neuroprotective signals. EMBO J. 21, 3317-3326.
12. Daniels M., Cereghetti G. M. and Brown D. R. (2001) Toxicity of novel C-terminal prion protein fragments and peptides harbouring disease-related C-terminal mutations. Eur. J. Biochem. 268, 6155-6164.
13. Demart S., Fournier J. G., Cremignon C., Frobert Y., Lamoury F., Marce D., Lasmézas C., Dormont D., Grassi J. and Deslys J.-P. (1999) New insight into abnormal prion protein using monoclonal antibodies. Biochem. Biophys. Res. Commun. 265, 652-657.
14. Enari M., Flechsig E. and Weissmann C. (2001) Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody. Proc. Natl. Acad. Sci. USA 98, 9295-9299.
15. Fenteany G., Standaert R., Lane W. S., Choi S., Corey E. J. and Schreiber S. L. (1995) Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 268, 726-731.
16. Forloni G., Angeretti N., Chiesa R., Monzani E., Salmona M., Bugiani O. and Tagliavini F. (1993) Neurotoxicity of a prion protein fragment. Nature 362, 543-546.
17. Ghetti B., Piccardo P., Frangione B., Bugiani O., Giaccone G., Young K., Prelli F., Farlow M. R., Dlouhy S. R. and Tagliavni F. (1996) Prion protein amyloidosis. Brain Pathol. 6, 127-145.
18. Heppner F. L., Musahl C., Arrighi I., Klein M. A., Rülicke T., Oesch B., Zinkemagel R. M., Kalinke U. and Aguzzi A. (1996) Prevention of scrapie pathogenesis by transgenic expression of anti-prion protein antibody. Science 294, 178-182.
19. Jobling M. F., Stewart L. R., White A. R., McLean C., Friedhuber A., Maher F. K. B., Masters C. L., Barrow C. J., Collins S. J. and Cappai R. (1999) The hydrophobic core sequence modulates the neurotoxic and secondary structure properties of the prion peptide 106-126. J. Neurochem. 73, 1557-1565.
20. Kurschner C. and Morgan J. I. (1995) The cellular prion protein (PrP) selectively binds to Bcl-2 in the yeast two-hybrid system. Mol. Brain Res. 30, 165-168.
21. Kuwahara C., Takeuchi A. M., Nishimura T., Haraguchi K., Kubosaki A., Matsumoto Y., Saeki K., matsumoto Y., Yokoyama T., Itohara S. and Onodera T. (1999) Prions prevent neuronal cell-line death. Nature 400, 225-226.
22. Ma J. and Lindquist S. (2001) Wild-type PrP and a mutant associated with prion disease are subject to retrograde transport and protea-some degradation. Proc. Natl. Acad. Sci. USA 98, 14955-14960.
23. Mallucci G. R., Ratté S., Asante E. A., Linehan J., Gowland I., Jefferys J. G. R. and Collinge J. (2002) Post-natal knockout of prion protein alters hippocampal CA1 properties, but does not result in neuro-degeneration. EMBO J. 21, 202-210.
24. Mouillet-Richard S., Ermonval M., Chebassier C., Laplanche J. L., Lehmann S., Launay J. M. and Kellermann O. (2000) Signal transduction through prion protein. Science 289, 1925-1928.
25. Peretz D., Williamson R. A., Kaneko K., Vergara J., Leclerc E., Schmitt-Ulms G., Mehlhom I. R., Legname G., Wormald M. R., Rudd P. M., Dwek R. A., Burton D. R. and Prusiner S. B. (2001) Antibodies inhibit prion propagation and clear cell cultures of prion infectivity. Nature 412, 739-743.
26. Prusiner S. B. (1998) Prions. Proc. Natl. Acad. Sci. USA 95, 13363-13383.
27. Schätzl H. M., Laszlo L., Holtzman D. M., Tatzelt J., DeArmond. S. J. R. I. W., Mobley W. C. and Prusiner S. B. (1997) A hypothalamic neuronal cell line persistently infected with scrapie prions exhibits apoptosis. J. Virol. 71, 8821-8831.
28. Vilette D., Andreoletti O., Archer F., Madelaine M. F., Vilotte J. L., Lehmann S. and Laude H. (2001) Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein. Proc. Natl. Acad. Sci. USA 98, 4055-4059.
29. Vincent B., Paitel E., Frobert Y., Lehmann S., Grassi J. and F. C. (2000) Phorbol ester-regulated cleavage of normal prion protein in HEK293 human cells and murine neurons. J. Biol. Chem. 275, 35612-35616.
30. Vincent B., Paitel E., Saftig P., Frobert Y., Hartmann D., de Strooper B., Grassi J., Lopez-Perez E. and Checler F. (2001) The disintegrins ADAM10 and TACE contribute to the constitutive and phorbolesters-regulated normal cleavage of the cellular prion protein. J. Biol. Chem. 276, 37743-37746.
31. Westaway D., DeArmond S. J., Cayetano-Canlas J., Groth D., Foster D., Yang S.-L., Torchia M., Carlson G. A. and Prusiner S. B. (1994) Degeneration of skeletal muscle, peripheral nerves and the central nervous system in transgenic mice overexpressing wild-type prion proteins. Cell 76, 117-129.
32. White A. R., Guirguis R., Brazier M. W., Jobling M. F., Hill A. F., Beyreuther K., Barrow C. J., Masters C. L., Colins S. J., and Cappai R. (2001) Sublethal concentrations of prion peptide PrP106-126 or the amyloid beta peptide of Alzheimer's disease activates expression of proapoptotic markers in primary cortical neurons. Neurobiol. Dis. 8, 299-316.
33. Yedidia Y., Horonchik L., Tzaban S., Yanai A. and Taraboulos A. (2001) Proteasomes and ubiquitin are involved in the turnover of the wildtype prion protein. EMBO J. 20, 5383-5391.
34. Yin X.-M., Oltvai Z. N. and Korsmeyer S. J. (1994) BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature 369, 321-323.
35. Zanata S. M., Lopes M. H., Mercadante A. F., Hajj G. N. M., Chiarini L. B., Nomizo R., Freitas A. R. O., Cabral A. L. B., Lee K. S., Juliano M. A., de Oliveira E., Jachieri S. G., Burlingame A., Huang L., Linden R., Brentani R. R. and Martins V. R. (2002) Stress-induced protein 1 is a cell surface ligand for cellular prion that triggers neuroprotection. EMBO J. 21, 3307-3316.
36. Zanusso G., Petersen R. B., Jin T., Jing Y., Kanoush R., Ferrari S., Gambetti P. and Singh N. (1999) Proteasomal degradation and N-terminal protease resistance of the codon 145 mutant prion protein. J. Biol. Chem. 274, 23396-23404.