Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS)

Zoological Science

Volumn 19, Issue 11, 2002, Pages 1257-1265

  Asami, Maki ^a,c   Nakatsuka, Tsuyoshi ^b   Hayashi, Tetsutaro ^c   Kou, Kenji ^a   Kagawa, Hiroaki ^a   Agata, Kiyokazu ^c  

^a OKAYAMA UNIVERSITY   (Japan)

^b UNIVERSITY OF HYOGO   (Japan)

^c RIKEN Center for Biosystems Dynamics Research   (Japan)

Author keywords

Extracellular matrix; FACS; Neuron; Planarian; Plasticity

Indexed keywords

FLUORESCENT DYE;

ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; BRAIN; CELL CULTURE; CULTURE TECHNIQUE; CYTOLOGY; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; HEAD; HISTOLOGY; METABOLISM; NERVE CELL; POLYMERASE CHAIN REACTION; TURBELLARIA;

ANIMALS; BRAIN; CELL CULTURE TECHNIQUES; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENT DYES; GENE EXPRESSION REGULATION; HEAD; NEURONS; ORGAN SPECIFICITY; PLANARIANS; POLYMERASE CHAIN REACTION;

ANIMALIA; PLANARIA; TURBELLARIA;

EID: 0036880246     PISSN: 02890003     EISSN: None     Source Type: Journal    
DOI: 10.2108/zsj.19.1257     Document Type: Article

References (25)

1. Agata K, Soejima Y, Kato K, Kobayashi C, Umesono Y, Watanabe K (1998) Structure of the planarian central nervous system (CNS) revealed by neuronal cell markers. Zool Sci 15: 433-440
2. Agata K, Watanabe K (1999) Molecular and cellular aspects of planarian regeneration. Semin Cell Dev Biol 10: 77-83
3. Cebrià F, Kobayashi C, Umesono Y, Nakazawa M, Mineta K, Ikeo K, Sánchez A, Kiyokazu A (2002a) FGFR-related gene noudarake restricts brain tissues to the head region of planarians. Nature, 419: 620-624
4. Cebrià F, Kudome T, Nakazawa M, Mineta K, Ikeo K, Gojobori T, Agata K (2002b) The expression of neural-specific genes reveals the structural and molecular complexity of the planarian central nervous system. Mech Dev 116: 199-204
5. Cebrià F, Nakazawa M, Mineta K, Ikeo K (2002c) Dissecting planarian central nervous system regeneration by the expression of neural-specific genes. Dev Growth Differ 44: 135-146
6. Cohen LB, Salazberg BM, Davila HV, Ross WN, Lansdowne D, Waggoner AS, Wang C H (1974) Changes in axon fluorescence during activity: molecular probes of membrane potential. J Membr Biol 19: 1-36
7. Dragsten PR, Watt WW (1978) Mechanism of the membrane potential sensitivity of the fluorescent membrane probe Merocyanine 540. Biochemistry 17: 5228-5240
8. Fitzakerley J L (2001) Survival and differentiation of neurons cultured from the mouse cochlear nucleus on extracellular matrix components. Neurosci Lett 316: 183-187
9. Kato K, Orii H, Watanabe K, Agata K (1999) The role of dorso-ventral interaction in the onset of planarian regeneration. Development 126: 1031-1040
10. Kobayashi C, Kobayashi S, Orii H, Watanabe K, Agata K (1998) Identification of two distinct muscles in the planarian Dugesia japonica by their expression of myosin heavy chain genes. Zool Sci 15: 855-863
11. Kobayashi C, Nogi T, Watanabe K, Agata K (1999) Ectopic pharynx arise by regional reorganization after anterior/posterior grafting in planarians. Mech Dev 89: 25-34
12. Koizumi O, Heimfeld S, Bode HR (1988) Plasticity in the nervous system of adult hydra. II. Conversion of ganglion cells of the body column into epidermal sensory cells of the hypostome. Dev Biol 129: 358-371
13. Lindroos P, Still MJ (1990) Laminin-, fibronectin- and collagen type IV-like substrates in Diphyllobothrium dendriticum- an immunocytochemical study. Acta Acad Aboensis B 50: 53-58
14. Lochter A, Taylor J, Braunewell KH, Holm J, Schachner M (1995) Control of neuronal morphology in vitro: interplay between adhesive substrate forces and molecular instruction. J Neurosci Res 42: 145-158
15. McAllister AK (2000) Cellular and Molecular Mechanisms of Dendrite Growth. Cereb Cortex 10: 963-973
16. Orii H, Kato K, Umesono Y, Agata K, Watanabe K (1999) The planarian HOM/HOX homeobox gene (Plox) expressed along anterior-posterior axis. Dev Biol 210: 456-468
17. Rieger R, Salvenmoser W, Legniti A, Reindl S, Adam H, Simonsberger P, Tyler S (1991) Organization and differentiation of the body-wall musculature in Macrostomum (Turbellaria, Macrostomidae). Hydrobiologia 227: 119-129
18. Ross WN, Salzberg B, Cohen LB, Davila HB, Waggoner AS, Wang C H (1977) Changes in absorption, fluorescence, dichroism, and birefringence in stained giant axons: optical measurement of membrane potential. J Membr Biol. 33: 141-183
19. Shibata N, Umesono Y, Orii H, Sakurai T, Watanabe K, Agata K (1999) Expression of vasa (vas)-related genes in germline cells and totipotent somatic stem cells of planarians. Dev Biol 206: 73-87
20. Tazaki A, Gaudieri S, Ikeo K, Gojobori T, Watanabe K, Agata K (1999) Neural network in planarian revealed by an antibody against planarian synaptotagmin homologue. Biochem Bioph Res Co 260: 426-432
21. Tasaki I, Hallett M, Carbone E (1973) Further studies of nerve membranes labeled with fluorescent probes. J Membr Biol 11: 353-376
22. Teshirogi W, Tohya K (1988) Primary tissue culture of freshwater planarians in a newly devised medium. Fortschr Zool 36: 91-96
23. Umesono Y, Watanabe K, Agata K (1997) A planarian orthopedia homolog is specifically expressed in the branch region of both the mature and regenerating brain. Dev Growth Differ 39: 723-727
24. Umesono Y, Watanabe K, Agata K (1999) Distinct structure domains in the planarian brain defined by the expression of evolutionarily conserved homeobox genes. Dev Genes Evol 209: 18-30
25. Zhang X, Sarras MP, Jr (1994) Cell-extracellular matrix interactions under in vitro conditions during interstitial cell migration in Hydra vulugaris. Development 120: 425-432