A RT-PCR assay combined with RFLP analysis for detection and differentiation of isolates of Pepino mosaic virus (PepMV) from tomato

European Journal of Plant Pathology

Volumn 108, Issue 9, 2002, Pages 887-892

  Martinez Culebras P V ^a   Lazaro A ^a   Abad Campos, P ^a   Jorda C ^a  

^a UNIVERSITAT POLITÈCNICA DE VALÈNCIA   (Spain)

Author keywords

Identification; Restriction analysis; Solanum muricatum; Specific primers

Indexed keywords

DETECTION METHOD; POLYMERASE CHAIN REACTION; VIRUS;

LYCOPERSICON ESCULENTUM; PEPINO MOSAIC VIRUS; SOLANUM; SOLANUM MURICATUM;

EID: 0036867188     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1021247220932     Document Type: Article

References (15)

1. French CJ, Bouthillier M, Bernardy M, Ferguson G, Sabourin M, Johnson RC, Masters C, Godkin S and Mumford R (2001) First report of Pepino mosaic virus in Canada and United States. Plant Disease 85: 1121
2. Gibbs A, Armstrong J, MacKenzie AM and Weiller GF (1998) The GPRIME package: Computer programs for identifying the best regions of aligned genes to target in nucleic acid hybridisation-based diagnostic tests, and their use with plant viruses. Journal of Virological Methods 74: 67-76
3. Jones RAC, Koenig R and Lesemann DE (1980) Pepino mosaic virus, a new potexvirus from pepino (Solanum muricatum). Annals of Applied Biology 94: 61-68
4. Jordá C, Lázaro-Pérez A, Font I, Lacasa A, Guerrero MM and Cano A (2000a) Nueva enfermedad en el tomate, Phytoma - España 119: 23-28
5. Jordá C, Lázaro-Pérez A, Martínez-Culebras PV, Medina V, Lacasa A, Guerrero MM, Cano A and Hita I (2000b) Avances en el conocimiento de un nuevo virus: El PepMV. Phytoma - España 121: 36-41
6. Jordá C, Lázaro-Pérez A, Martínez-Culebras PV and Abad-Campos P (2001a) First report of Pepino mosaic virus on tomato in Spain. Plant Disease 85: 1292
7. Jordá C, Lázaro-Pérez A, Martínez-Culebras PV and Lacasa A (2001b) First report of Pepino mosaic virus on natural hosts. Plant Disease 85: 1292
8. Kumar S, Tamura K, and Nei M (eds) (1993) MEGA: Molecular Evolutionary Genetics Analysis, V. 1.0. The Pennsylvania State University, University Park, Pennsylvania
9. Lázaro-Pérez A, Martínez-Culebras PV, Abad-Campos P and Jordá C (2002) A new disease in tomato crops caused by Pepino mosaic virus (PepMV). Diagnosis by different techniques. Proceedings of the 5th Congress of the European Foundation for Plant Pathology, pp 239-241
10. Lesemann DE, Dalchow J, Winter S and Pfeilstetter E (2000) Occurrence of Pepino mosaic virus in European tomato crops: Identification, etiology and epidemiology. Mitteilungen aus der Biologischen Bundesanstalt für Land-und Forstwirschaft, Berlin-Dahlem 376: 323
11. Mumford RA and Metcalfe EJ (2001) The partial sequencing of the genomic RNA of a UK isolate of Pepino mosaic virus and the comparison of the coat protein sequence with other isolates from Europe and Peru. Archives of Virology 146: 2455-2460
12. Saitou N and Nei M (1987) The neighbour-joining method: A new method for reconstruction phylogenetic trees. Molecular Biology and Evolution 4: 406-425
13. Thompson JD, Higgins DG, Gibson TJ and Clustal W (1994): Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680
14. van der Vlugt RAA, Stijger CCMM, Verhoeven J and Lesemann DE (2000) First report of Pepino mosaic virus on tomato. Plant Disease 84: 103
15. Wright D and Mumford R (1999) Pepino mosaic virus (PepMV). First records in tomato in the United Kingdom. Plant Disease Notice, 89, CSL York