Production of recombinant human tissue transglutaminase using the baculovirus expression system, and its application for serological diagnosis of coeliac disease

European Journal of Gastroenterology and Hepatology

Volumn 14, Issue 11, 2002, Pages 1217-1223

  Osman, Awad A ^a   Richter, Thomas ^a   Stern, Martin ^b   Conrad, Karsten ^c   Henker, Jobst ^d   Brandsch, Corinna ^e   Zimmer, Klaus Peter ^f   Mothes, Thomas ^a  

^a UNIVERSITY HOSPITAL LEIPZIG   (Germany)

^b UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^c DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^d UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

^e MARTIN LUTHER UNIVERSITÄT HALLE WITTENBERG   (Germany)

^f UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Antibodies; Baculovirus expression system; Coeliac disease; Dermatitis herpetiformis; Endomysium antibodies; Recombinant human tissue transglutaminase

Indexed keywords

AUTOANTIGEN; ENDOMYSIUM ANTIBODY; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; RECOMBINANT ENZYME;

ADOLESCENT; ADULT; AGED; ANTIBODY TITER; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CARBOXY TERMINAL SEQUENCE; CELIAC DISEASE; CELL LYSATE; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYSTIC FIBROSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DIFFERENTIAL DIAGNOSIS; ENTERITIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE EXPRESSION; GUINEA PIG; HUMAN; HUMAN TISSUE; INSECT CELL; MALE; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACULOVIRIDAE; BLOTTING, WESTERN; CELIAC DISEASE; CELLS, CULTURED; CHILD; CHILD, PRESCHOOL; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENETIC VECTORS; HUMANS; MIDDLE AGED; RECOMBINANT PROTEINS; TRANSFECTION; TRANSGLUTAMINASES;

EID: 0036865727     PISSN: 0954691X     EISSN: None     Source Type: Journal    
DOI: 10.1097/00042737-200211000-00009     Document Type: Article

References (40)

1. Dieterich W, Ehnis T, Bauer M, Donner P, Volta U, Riecken EO, et al. Identification of tissue transglutaminase as the autoantigen of coeliac disease. Nature Med 1997; 3:797-801.
2. Lorand L, Conrand SM. Transglutaminases. Mol Cell Biochem 1984; 58:9-35.
3. Dieterich W, Laag E, Schöpper H, Volta U, Ferguson A, Gillett H, et al. Autoantibodies to tissue transglutaminase as predictors of celiac disease. Gastroenterology 1998; 115:1317-1321.
4. Brusco G, Izzi L, Corazza GR. Tissue transglutaminase antibodies for coeliac disease screening. Ital J Gastroenterol Hepatol 1998; 30:496-497.
5. Amin M, Eckhardt T, Kapitza S, Fleckenstein B, Jung G, Seissler J, et al. Correlation between tissue transglutaminase antibodies and endomysium antibodies as diagnostic markers of coeliac disease. Clin Chim Acta 1999; 282:219-225.
6. Biagi F, Ellis HJ, Yiannakou JY, Brusco G, Swift GL, Smith PM, et al. Tissue transglutaminase antibodies in celiac disease. Am J Gastroenterol 1999; 94:2187-2192.
7. Dieterich W, Laag E, Bruckner-Tudermann L, Reunala T, Karpati S, Zagoni T, et al. Antibodies to tissue transglutaminase as serologic markers in patients with dermatitis herpetiformis. J Invest Dermatol 1999; 113:133-136.
8. Lock RJ, Pitcher MCL, Unsworth DJ. IgA anti-tissue transglutaminase as a diagnostic marker of gluten sensitive enteropathy. J Clin Pathol 1999; 52:274-277.
9. Miller A, Elliott PR, Paspaliaris W, D'Apice A. Anti-transglutaminase antibodies and coeliac disease. Aust NZ J Med 1999; 29:239-242.
10. Vitoria JC, Arrieta A, Arranz C, Ayesta A, Sojo A, Maruri N, et al. Antibodies to gliadin, endomysium, and tissue transglutaminase for the diagnosis of celiac disease. J Ped Gastroenterol Nutr 1999; 29:571-574.
11. Sulkanen S, Haltunen T, Laurila K, Kolho K-L, Korponay-Szabò IR, Sarnesto A, et al. Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease. Gastroenterology 1998; 115:1322-1328.
12. Troncone R, Maurano F, Rossi M, Micillo M, Greco L, Auricchio R, et al. IgA antibodies to tissue transglutaminase: An effective diagnostic test for celiac disease. J Pediatr 1999; 134:166-171.
13. Koop I, Ilchmann R, Izzi L, Adragna A, Koop H, Barthelmes H. Detection of autoantibodies against tissue transglutaminase in patients with celiac disease and dermatitis herpetiformis. Am J Gastroenterol 2000; 95:2009-2014.
14. Salmaso C, Ocmant A, Pesce G, Altrinetti V, Montagna P, Descalzi D, et al. Comparison of ELISA for tissue transglutaminase autoantibodies with endomysium antibodies in pediatric and adult patients with celiac disease. Allergy 2001; 56:544-547.
15. Dahele AVM, Aldhous MC, Humphreys K, Ghosh S. Serum IgA tissue transglutaminase antibodies in coeliac disease and other gastrointestinal diseases. Q J Med 2001; 94:195-205.
16. Fabiani E, Catassi C. The serum IgA class anti-tissue transglutaminase antibodies in the diagnosis and follow-up of coeliac disease. Results of an international multicentre study. Eur J Gastroenterol Hepatol 2001; 13:659-665.
17. Ikura K, Nasu T, Yokota H, Tsuchiya Y, sasaki R, Chiba H. Amino acid sequence of guinea pig liver transglutaminase from its cDNA sequence. Biochemistry 1988; 27:2898-2905.
18. Gentile V, Saydak M, Chiocca EA, Akande O, Birckbichler PJ, lee KN, et al. Isolation and characterisation of cDNA clones to mouse macrophage and human endothel cell tissue transglutaminases. J Biol Chem 1991; 266:478-483.
19. Hansson T, Dahlbom I, Hall J, Holtz A, Elfman L, Dannaeus A, et al. Antibody reactivity against human and guinea pig tissue transglutaminase in children with celiac disease. J Pediatr Gastroenterol Nutr 2000; 30:379-384.
20. Bazzigaluppi E, Lampasona V, Barera G, Venerando A, Bianchi C, Chiumello G, et al. Comparison of tissue transglutaminase-specific assays with established antibody measurements for coeliac disease. J Autoimmunity 1999; 12:51-56.
21. Seissler J, Boms S, Wohlrab U, Morgenthaler NG, Mothes T, Boehm BO, et al. Antibodies to human recombinant tissue transglutaminase measured by radioligand assay: Evidence for high diagnostic sensitivity for celiac disease. Horm Metab Res 1999; 31:375-379.
22. Bao F, Yu L, Babu S, Wang T, Hoffenberg EJ, Rewers M, et al. One third of HLA DQ2 homozygous patients with Type 1 diabetes express celiac disease-associated transglutaminase autoantibodies. J Autoimmunity 1999; 13:143-148.
23. Williams AJK, Annis P, Lock RJ, Unsworth DJ, Gale EAM, Bingley PJ. Evaluation of a high-throughput second antibody radiobinding assay for measuring IgA antibodies to human tissue transglutaminase. J Immunol Meth 1999; 228:81-85.
24. Hoffenberg EJ, Bao F, Eisenbarth, Uhlborn C, Hass JE, Sokol RJ, et al. Transglutaminase antibodies in children with a genetic risk for celiac disease. J Pediatr 2000; 137:356-360.
25. Bonamico M, Tiberti C, Picarelli A, Mariani P, Rossi D, Cipolletta E, et al. Radioimmunoassay to detect antitransglutaminase autoantibodies is the most sensitive and specific screening method for celiac disease. Am J Gastroenterol 2001; 96:1536-1540.
26. Sardy M, Odenthal U, Karpati S, Paulsson M, Smyth N. Recombinant human tissue transglutaminase ELISA for the diagnosis of gluten-sensitive enteropathy. Clin Chem 1999; 45:2142-2149.
27. Sblattero D, Berti I, Trevisiol C, Marzari R, Tommasini A, Bradbury A, et al. Human recombinant tissue transglutaminase ELISA: an innovative diagnostic assay for celiac disease. Am J Gastroenterol 2000; 95:1253-1257.
28. Walker-Smith JA, Guandalini S, Schmitz J, Shmerling DH, Visakorpi JK. Revised criteria for diagnosis of coeliac disease. Arch Dis Child 1990; 65:909-911.
29. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1979; 227:680-685.
30. Kyhse-Anderson J. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 1984; 10:303-309.
31. Osman AA, Uhlig H, Thamm B, Schneider-Mergener J, Mothes T. Use of phage display technique for detection of epitopes recognized by polyclonal rabbit gliadin antibodies. FEBS Letters 1998; 433:103-107.
32. Hällström A. Comparison of IgA-class reticulin and endomysium antibodies in coeliac disease and dermatitis herpetiformis. Gut 1989; 30:1225-1232.
33. Stern M. Comparative evaluation of serologic tests for celiac disease: A European initiative toward standardization. J Ped Gastroenterol Nutr 2000; 31:513-519.
34. Aldersley MA, Hamlin PJ, Jones PF, Markham AF, Robinson PA, Howdle PD. No polymorphism in the tissue transglutaminase gene detected in coeliac disease patients. Scand J Gastroentrol 2000; 35:61-63.
35. Leon F, Camarero C, Pena R, Eiras P, Sanchez L, Baragano M, et al. Anti-transglutaminase IgA ELISA: Clinical potential and drawbacks in celiac disease diagnosis. Scand J Gastroenterol 2001; 36:849-853.
36. Clemente MG, Musu MP, Frau F, Lucia C, de Virgilis S. Antitissue transglutaminase antibodies outside celiac disease. J Pediatr Gastroenterol Nutr 2002; 34:31-34.
37. Uhlig HH, Lichtenfeld J, Osman AA, Richter T, Mothes T. Evidence for existence of coeliac disease autoantigens apart from tissue transglutaminase. Eur J Gastroenterol Hepatol 2000; 12:1017-1020.
38. Troncone R, Greco L, Mayer M, Paparo F, Caputo N, Micillo M, et al. Latent and potential coeliac disease. Acta Paed 1996; 412(Suppl.):10-14.
39. Kaukinen K, Mäki M, Partanen J, Sievänen H, Collin P. Celiac disease without villous atrophy. Revision of criteria called for. Dig Dis Sci 2001; 46:879-887.
40. Collin P, Helin H, Hällström O, Karvonen A-L. Follow-up of patients in reticulin and gliadin antibody tests with normal small-bowel biopsy findings. Scand J Gastroenterol 1993; 28:595-598.