Alginate production by Azotobacter vinelandii mutants altered in poly-β-hydroxybutyrate and alginate biosynthesis

Journal of Industrial Microbiology and Biotechnology

Volumn 29, Issue 5, 2002, Pages 209-213

  Pena C ^a   Miranda, L ^a   Segura, D ^a   Nunez C ^a   Espin G ^a   Galindo, Enrique ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

A. vinelandii; Alginate; Molecular weight; Mutants

Indexed keywords

ALGINIC ACID; BACTERIAL ENZYME; GUANOSINE DIPHOSPHATE MANNOSE DEHYDROGENASE; OXIDOREDUCTASE; POLY(3 HYDROXYBUTYRIC ACID); UNCLASSIFIED DRUG;

ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL GENE; BACTERIUM MUTANT; GENE ALGD; GENE MUC26; GENE MUTATION; GENE PHBBAC; GENE PHBR; MOLECULAR WEIGHT; NONHUMAN; OPERON; VISCOSITY;

ALGINATES; AZOTOBACTER VINELANDII; CLONING, MOLECULAR; DNA, BACTERIAL; GLUCURONIC ACID; HEXURONIC ACIDS; HYDROXYBUTYRATES; MUTATION; POLYESTERS;

AZOTOBACTER; AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0036851370     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.7000310     Document Type: Article

References (18)

1. Brivonese A and I Sutherland. 1989. Polymer production by a mucoid strain of Azotobacter vinelandii in batch culture. Appl Microbiol Biotechnol 30: 97-102.
2. Byrom D. 1987. Polymer synthesis by microorganisms: Technology and economics. Trends Biotechnol 5: 246-250.
3. Campos M, J Martínez-Salazar, L Lloret, S Moreno, C Núñez, G Espín and G Soberón-Chávez. 1996. Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii. J Bacteriol 178: 1793-1799.
4. Chen W, J Chen, S Chang and C Su. 1985. Bacterial alginate produced by a mutant of Azotobacter vinelandii. Appl Environ Microbiol 49: 543-546.
5. De Lorenzo V, M Herrero, V Jakubzic and K Timmis. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J Bacteriol 172: 6568-6572.
6. Gacesa P. 1998. Bacterial alginates biosynthesis - Recent progress and future prospects. Rev Microbiol 114: 1133-1142.
7. Horan N, T Jarman and E Dawes. 1981. Effects of carbon sources and inorganic phosphate concentration on the production of alginic acid by a mutant of Azotobacter vinelandii and on the enzymes involved in its biosynthesis. J Gen Microbiol 127: 185-191.
8. Martínez P, J Guzmán and G Espín. 1997. A mutation impairing alginate production increased accumulation of poly-β-hydroxybutyrate in Azotobacter vinelandii. Biotechnol Lett 19: 909-912.
9. Martinsen A, G Skjak-Braek, O Smidsrod, F Zanetti and S Paoletti. 1991. Comparison of different methods for determination of molecular weight and molecular weight distributions of alginates. Carbohydr Polym 15: 171-193.
10. Mc Comb A and R Mc Cready. 1957. Determination of acetyl in pectin and in acetylated carbohydrate polymers. Anal Chem 29(5): 819-821.
11. Miller JH. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. Peña C, N Campos and E Galindo. 1997. Changes in the molecular mass distributions, broth viscosity and morphology of Azotobacter vinelandii cultured in shake flasks. Appl Microbiol Biotechnol 48: 510-515.
13. Peña C, M Trujillo-Roldán and E Galindo. 2000. Influence of dissolved oxygen tension and agitation speed on alginate production and its molecular weight in cultures of Azotobacter vinelandii. Enzyme Microb Technol 27(6): 390-398.
14. Peralta-Gil M, D Segura, J Guzmán, L Servín-González and G Espín. 2002. Expression of the Azotobacter vinelandii poly-β-hydroxybutyrate biosynthetic phbBAC operon is driven by two overlapping promoters and is dependent on the transcriptional activator PhbR. J Bacteriol 184 (in press).
15. Rehm B and S Valla. 1997. Bacterial alginates. Biosynthesis and applications. Appl Microbiol Biotechnol 48(3): 281-288.
16. Sambrook J, E Fritsch and T Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Skjak-Braek G, H Grasdalem and L Larsen. 1986. Monomer sequence and acetylation pattern in some bacterial alginates. Carbohydr Res 154: 239-250.
18. Stover C, X Pham, A Erwin, D Mizoguchi, D Warrener, M Hickey, F Brinkman, W Hufnagle, D Kowalic, M Lagrou, R Garber, L Goltry, E Tolentino, S Westbrook, Y Yvan, L Brody, S Coulter, R Folger, A Kass, K Larbig, K Smith, D Spencer, H Wong, Z Wu, I Paulsen, J Reizer, M Saier, R Hancock, S Lory and M Olson. 2000. Complete genome sequence of Pseudomonas aeruginosa PA01 an opportunistic pathogen. Nature 406: 959-964.