Expression in bacteria of the gene encoding the gp43 antigen of Paracoccidioides brasiliensis: Immunological reactivity of the recombinant fusion proteins

Clinical and Diagnostic Laboratory Immunology

Volumn 9, Issue 6, 2002, Pages 1200-1204

  Diniz, Susana N ^a   Carvalho, Kátia C ^a   Cisalpino, Patricía S ^a   Silveira, José F ^a   Travassos, Luiz R ^a   Puccia, Rosana ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; GLUTATHIONE TRANSFERASE; GLYCOPROTEIN GP 43; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ADOLESCENT; ADULT; AGED; AMINO TERMINAL SEQUENCE; ANTIGENICITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CHRONIC LUNG DISEASE; DNA VECTOR; ESCHERICHIA COLI; FEMALE; FUNGAL GENE; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOREACTIVITY; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARACOCCIDIOIDES BRASILIENSIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHOOL CHILD; SERUM; SOUTH AMERICAN BLASTOMYCOSIS;

ANIMALS; ANTIGENS, FUNGAL; ESCHERICHIA COLI; IMMUNOBLOTTING; PARACOCCIDIOIDES; PARACOCCIDIOIDOMYCOSIS; RABBITS; RECOMBINANT FUSION PROTEINS;

EID: 0036843812     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.9.6.1200-1204.2002     Document Type: Article

References (27)

1. Almeida, I. C., D. C. A. Neville, A. Mehlert, A. Treumann, M. A. J. Ferguson, J. O. Previato, and L. R. Travassos. 1996. Structure of the N-linked oligosaccharides of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis. Glycobiology 6:507-515.
2. Almeida, I. C., E. G. Rodrigues, and L. R. Travassos. 1994. Chemiluminescent immunoassays: Discrimination between the reactivities of natural and human patient antibodies with antigens of eukaryotic pathogens, Trypanosoma cruzi and Paracoccidioides brasiliensis. J. Clin. Lab. Anal. 8:424-431.
3. Blotta, M. H. S. L., and Z. P. Camargo. 1993. Immunological response to cell-free antigens of Paracoccidioides brasiliensis: Relationship with clinical forms of paracoccidioidomycosis. J. Clin. Microbiol. 31:671-676.
4. Bueno, J. P., M. J. Mendes Giannini, G. M. Del Negro, C. M. Assis, C. K. Takiguti, and M. A. Shikanai Yasuda. 1997. IgG, IgM and IgA antibody response for the diagnosis and follow-up of paracoccidioidomycosis: Comparison of counterimmunoelectrophoresis and complement fixation J. Med. Vet. Mycol. 35:213-217.
5. Camargo, Z. P., J. L. Gesztesi, E. C. O. Saraiva, C. P. Taborda, A. P. Vicentini, and J. D. Lopes. 1994. Monoclonal antibody capture enzyme immunoassay for detection of Paracoccidioides brasiliensis antibodies in paracoccidioidomycosis. J. Clin. Microbiol. 32:2377-2381.
6. Cisalpino, P. S., R. Puccia, L. M. Yamauchi, M. I. N. Cano, J. F. Silveira, and L. R. Travassos. 1996. Cloning, characterization, and epitope expression of the major diagnostic antigen of Paracoccidioides brasiliensis. J. Biol. Chem. 271:4553-4560.
7. Cisalpino, P. S., J. F. Silveira, and L. R. Travassos. 1994. RNA and DNA isolation from Paracoccidioides brasiliensis yeast cells: Establishment of cDNA and genomic libraries, and PCR amplification, p. 461-467. In B. Maresca and G. S. Kobayashi (ed.), Molecular Biology of Pathogenic Fungi, A Laboratory Manual, Telos Press, New York, N.Y.
8. Franco, M. 1987. Host-parasite relationships in paracoccidioidomycosis. J. Med. Vet. Mycol. 25:5-18.
9. Gesztesi, J. L., R. Puccia, L. R. Travassos, A. P. Vicentini, M. F. Franco, and J. D. Lopes. 1996. Monoclonal antibodies against the 43,000 Da glycoprotein from Paracoccidioides brasiliensis modulate laminin-mediated fungal adhesion to epithelial cells and pathogenesis. Hybridoma 15:415-422.
10. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 224:680-685.
11. Mendes-Giannini, M. J. S., J. P. Bueno, M. A. Shikanai-Yasuda, A. M. S. Stolf, A. Masuda, V. Amato Neto, and A. W. Ferreira. 1990. Antibody response to the 43 kDa glycoprotein of Paracoccidioides brasiliensis as a marker for the evaluation of patients under treatment. Am. J. Trop. Med. Hyg. 43:200-206.
12. Morais, F. V., T. F. Barros, M. K. Fukada, P. S. Cisalpino, and R. Puccia. 2000. Polymorphism in the gene coding for the immunodominant antigen gp43 from the pathogenic fungus Paracoccidioides brasiliensis. J. Clin. Microbiol. 38:3960-3966.
13. Moura-Campos, C., J. L. Gesztesi, A. P. Vincentini, J. D. Lopes, and Z. P. Camargo. 1995. Expression and isoforms of gp43 in different strains of Paracoccidioides brasiliensis. J. Med. Vet. Mycol. 33:223-227.
14. Pinto, A. R., R. Puccia, S. N. Diniz, M. F. Franco, and L. R. Travassos. 2000. DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from Paracoccidioides brasiliensis. Vaccine 18:3050-3058.
15. Puccia, R., and L. R. Travassos. 1991. Forty-three-kilodalton glycoprotein from Paracoccidioides brasiliensis: Immunochemical reactions with sera from patients with paracocci dioidomycosis, histoplasmosis, and Jorge Lobo's disease. J. Clin. Microbiol. 29:1610-1615.
16. Puccia, R., and L. R. Travassos. 1991. The 43-kDa glycoprotein from Paracoccidioides brasiliensis and its deglycosylated form: Excretion and susceptibility to proteolysis. Arch. Biochem. Biophys. 289:298-302.
17. Puccia, R., S. Schenkman, P. A. J. Gorin, and L. R. Travassos. 1986. Exocellular components of Paracoccidioides brasiliensis: Identification of a specific antigen. Infect. Immun. 53:193-203.
18. Puccia, R., D. T. Takaoka, and L. R. Travassos. 1991. Purification of the 43 kDa glycoprotein from exocellular components excreted by Paracoccidioides brasiliensis in liquid culture (TOM medium). J. Med. Vet. Mycol. 29:57-60.
19. Restrepo, A., M. Restrepo, F. Restrepo, L. H. Aristizábal, L. H. Moncada, and H. Velez. 1978. Immune responses in paracoccidioidomycosis. A controlled study of 16 patients before and after treatment. Sabouraudia 16:151-163.
20. Rodrigues, E. G., and L. R. Travassos. 1994. Nature of the reactive epitopes in Paracoccidioides brasiliensis polysaccharide antigen. J. Med. Vet. Mycol. 32:77-81.
21. Saraiva, E. C. O., A. Altemani, M. F. Franco, C. S. Unterkircher, and Z. P. Camargo. 1996. Paracoccidioides brasiliensis-gp43 used as paracoccidioidin. J. Vet. Med. Mycol. 34:155-161.
22. Souza, M. C., J. L. Gesztesi, A. R. Souza, J. Z. Moraes, J. D. Lopes, and Z. P. Camargo. 1997. Differences in reactivity of paracoccidioidomycosis sera with gp43 isoforms. J. Med. Vet. Mycol. 35:13-18.
23. Taba, M. R. M., J. F. Silveira, L. R. Travassos, and S. Schenkman. 1989. Expression in Escherichia coli of a gene coding for epitopes of a diagnostic antigen of Paracoccidioides brasiliensis. Exp. Mycol. 13:223-230.
24. Taborda, C. P., and Z. P. Camargo. 1993. Diagnosis of paracoccidioidomycosis by passive hemagglutination assay for antibody using a purified and specific antigen gp43. J. Med. Vet. Mycol. 31:155-160.
25. Taborda, C. P., M. A. Juliano, R. Puccia, M. Franco, and L. R. Travassos. 1998. Mapping of the T-cell epitope in the major 43-kilodalton glycoprotein of Paracoccidioides brasiliensis which induces a Th-1 response protective against fungal infection in Balb/c mice. Infect. Immun. 66:786-793.
26. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
27. Vicentini, A. P., J. L. Gesztesi, M. F. Franco, W. Souza, J. Z. Moraes, L. R. Travassos, and J. D. Lopes. 1994. Binding of Paracoccidioides brasiliensis to laminin through surface glycoprotein gp43 leads to enhancement of fungal pathogenesis. Infect. Immun. 62:1465-1469.