PCR-based detection of Bacillus anthracis in formalin-fixed tissue from a patient receiving ciprofloxacin

Journal of Clinical Microbiology

Volumn 40, Issue 11, 2002, Pages 4360-4362

  Levine, Steven M ^a   Perez Perez, Guillermo ^a   Olivares, Asalia ^a   Yee, Herman ^a   Hanna, Bruce A ^a   Blaser, Martin J ^a  

^a Department of Medicine ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CIPROFLOXACIN; FORMALDEHYDE; PARAFFIN;

ADULT; ANTHRAX; ANTIBIOTIC THERAPY; ARTICLE; BACILLUS ANTHRACIS; BACTERIUM DETECTION; BIOPSY TECHNIQUE; CASE REPORT; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; HUMAN; HUMAN TISSUE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; TISSUE FIXATION;

ADULT; ANTHRAX; ANTI-INFECTIVE AGENTS; BACILLUS ANTHRACIS; BIOPSY; CIPROFLOXACIN; FIXATIVES; FORMALDEHYDE; HUMANS; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; TISSUE FIXATION;

ANTHRAX; BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0036841046     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.11.4360-4362.2002     Document Type: Article

References (19)

1. Bowen, J. E., and C. P. Quinn. 1999. The native virulence plasmid combination affects the segregational stability of a theta-replicating shuttle vector in Bacillus anthracis var. New Hampshire. J. Appl. Microbiol. 87:270-278.
2. Bush, L. M., B. H. Abrams, A. Beall, and C. C. Johnson. 2001. Index case of fatal inhalational anthrax due to bioterrorism in the United States. N. Engl. J. Med. 345:1607-1610.
3. Centers for Disease Control and Prevention. 2001. Update: Investigation of bioterrorism-related anthrax, 2001. Morb. Mortal. Wkly. Rep. 50:1008-1010.
4. Coombs, N. J., A. C. Gough, and J. N. Primrose. 1999. Optimisation of DNA and RNA extraction from archival formalin-fixed tissue. Nucleic Acids Res. 27:e12-e16.
5. Fouet, A., and M. Mock. 1996. Differential influence of the two Bacillus anthracis plasmids on regulation of virulence gene expression. Infect. Immun. 64:4928-4932.
6. Galiagher, T. C., and B. E. Strober. 2001. Cutaneous Bacillus anthracis infection. N. Engl. J. Med. 345:1646-1647.
7. Green, B. D., L. Battisti, T. M. Koehler, C. B. Thorne, and B. E. Ivins. 1985. Demonstration of a capsule plasmid in Bacillus anthracis. Infect. Immun. 49:291-297.
8. Inglesby, T. V., D. A. Henderson, J. G. Bartlett, et al. 1999. Anthrax as a biological weapon: medical and public health management. JAMA 281:1635-1645.
9. Jackson, P. J., M. E. Hugh-Jones, D. M. Adair, et al. 1999. PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims: The presence of multiple Bacillus anthracis strains in different victims. Proc. Natl. Acad. Sci. USA 95:1224-1229.
10. Jernigan, J. A., D. S. Stephens, D. A. Ashford, et al. 2001. Bioterrorism-related inhalational anthrax: The first ten cases reported in the United States. Emerg. Infect. Dis. 7:933-944.
11. Mikesell, P., B. E. Ivins, J. D. Ristroph, and T. M. Dreier. 1983. Evidence for plasmid-mediated toxin production in Bacillus anthracis. Infect. Immun. 39:371-376.
12. Mock, M., and A. Fouet. 2001. Anthrax. Annu. Rev. Microbiol. 55:647-671.
13. Patra, G., P. SyIvestre, V. Ramisse, J. Therasse, and J.-L. Guesdon. 1996. Specific oligonucleotide primers for rapid identification of Bacillus anthracis strains. Salisbury Med. Bull. 87(Special Suppl):45-46.
14. Patra, G., P. Sylvestre, V. Ramisse, J. Therasse, and J. L. Guesdon. 1996. Isolation of a specific chromosomal DNA sequence of Bacillus anthracis and its possible use in diagnosis. FEMS Immunol. Med. Microbiol. 15:223-231.
15. Patra, G., J. Vaissaire, M. Weber-Levy, C. Le Doujet, and M. Mock. 1998. Molecular characterization of Bacillus strains involved in outbreaks of anthrax in France in 1997. J. Clin. Microbiol. 36:3412-3414.
16. Ramisse, V., G. Patra, J. Vaissaire, and M. Mock. 1999. The Ba813 chromosomal DNA sequence effectively traces the whole Bacillus anthracis community. J. Appl. Microbiol. 87:224-228.
17. Uchida, I., T. Sekizaki, K. Hashimoto, and N. Terakado. 1985. Association of the encapsulation of Bacillus anthracis with a 60 megadalton plasmid. J. Gen. Microbiol. 131:363-367.
18. Uhl, J. R., C. A. Bell, L. M. Sloan, M. J. Espy, T. F. Smith, J. E. Rosenblatt, and F. R. Cockerill III. 2002. Application of rapid-cycle real-time polymerase chain reaction for the detection of microbial pathogens: The Mayo-Roche Rapid Anthrax Test. Mayo Clin. Proc. 77:673-680.
19. Wright, D., and M. Manos. 1990. Sample preparation from paraffin-embedded tissues, p. 153. In M. Innis, D. Gelfant, J. Sninsky, and T. White (ed.), PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, Calif.