Novel allelic variants of mycobacteria isolated in Brazil as determined by PCR-restriction enzyme analysis of hsp65

Journal of Clinical Microbiology

Volumn 40, Issue 11, 2002, Pages 4191-4196

  Da Silva Rocha, A ^a   Barreto, A M Werneck ^a   Dias Campos, C E ^a   Villas Boas Da Silva M ^a   Fonseca, L ^a   Saad, M H ^a   Degrave, W M ^a   Suffys, P N ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ALGORITHM; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; BRAZIL; CONTROLLED STUDY; ENZYME ANALYSIS; GENETIC VARIABILITY; GENOTYPE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM FLAVESCENS; MYCOBACTERIUM GORDONAE; MYCOBACTERIUM INTRACELLULARE; MYCOBACTERIUM SCROFULACEUM; MYCOBACTERIUM TERRAE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRAIN DIFFERENCE; STRAIN IDENTIFICATION;

ALLELES; BACTERIAL PROTEINS; BRAZIL; CHAPERONINS; DATABASES, GENETIC; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM; MYCOBACTERIUM INFECTIONS; POLYMERASE CHAIN REACTION; VARIATION (GENETICS);

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM FLAVESCENS; MYCOBACTERIUM FORTUITUM; MYCOBACTERIUM GORDONAE; MYCOBACTERIUM INTRACELLULARE; MYCOBACTERIUM SCROFULACEUM; MYCOBACTERIUM TERRAE; UNCULTURED ACTINOMYCETE;

EID: 0036840124     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.11.4191-4196.2002     Document Type: Article

References (25)

1. Alcaide, F., I. Richter, C. Bernasconi, B. Springer, C. Hagenau, R. Schultze-Robbecke E. Tortoli, R. Martin, E. C. Bottger, and A. Telenti. 1997. Heterogeneity and clonality among isolates of Mycobacterium kansasii: Implications for epidemiological and pathogenicity studies. J. Clin. Microbiol. 35:1959-1964.
2. Brunello, F., M. Ligozzi, E. Cristelli, S. Bonora, E. Tortoli, and R. Fontana. 2001. Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene. J. Clin. Microbiol. 39:2799-2806.
3. Da Silva, F. C., S. Y. M. Ueki, D. C. P. Geiger, and S. C. Leao. 2001. Hsp65 PCR-restriction enzyme analysis (PRA) for identification of mycobacteria in the clinical laboratory. Rev. Inst. Med. Trop. Sao Paulo 43:25-28.
4. Da Silva Rocha, A., C. da Costa Leite, H. Magarinos Torres, A. B. de Miranda, M. Q. Pires Lopes, W. M. Degrave, and P. N. Suffys. 1999. Use of PCR-restriction fragment polymorphism analysis of the hsp65 gene for rapid identification of mycobacteria in Brazil. J. Microbiol. Methods 37:223-229.
5. Devallois, A., M. Picardeau, C. N. Paramasivan, V. Vincent, and N. Rastogi. 1997. Molecular characterization of Mycobacterium avium complex isolates giving discordant results in AccuProbe tests by PCR-restriction enzyme analysis, 16S rRNA gene sequencing, and DT1-DT6 PCR. J. Clin. Microbiol. 35:2767-2772.
6. Devallois, A., K. Seng Goh, and N. Rastogi. 1997. Rapid identification of mycobacteria to the species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species. J. Clin. Microbiol. 35:2969-2973.
7. Huys, G., L. Rigouts, K. Chemlal, F. Portaels, and J. Swings. 2000. Evaluation of amplified fragment length polymorphism analysis for inter- and intraspecific differentiation of Mycobacterium bovis, M. tuberculosis, and M. ulcerans. J. Clin. Microbiol. 38:3675-3680.
8. Kamala, T., C. N. Paramasivan, D. Herbert, P. Venkatesan, and R. Prabhakar. 1994. Isolation and identification of environmental mycobacteria in the Mycobacterium bovis BCG trial area of South India. Appl. Environ. Microbiol. 60:2180-2183.
9. Kent, P. T., and G. P. Kubica. 1985. Public Health Mycobacteriology. A Guide for the Level III Laboratory. Centers for Disease Control, Atlanta, Ga.
10. Khosravi, A. D., J. L. Stanford, H. D. Donoghue, and G. A. W. Rook. 1997. Variation within Mycobacterium scrofulaceum. J. Appl. Microbiol. 83:596-602.
11. Leao, S. C., M. R. S. Briones, M. P. Sircili, S. C. Balian, N. Mores, and J. S. Ferreira-Neto. 1999. Identification of two novel Mycobacterium avium allelic variants in pig and human isolates from Brazil by PCR-restriction enzyme analysis. J. Clin. Microbiol. 37:2592-2597.
12. Legrand, E., K. S. Goh, C. Sola, and N. Rastogi. 2000. Description of a novel Mycobacterium simiae allelic variant isolated from Caribbean AIDS patients by PCR-restriction enzyme analysis and sequencing of hsp65 gene. Mol. Cell. Probes 14:355-363.
13. Miller, N., S. Infante, and T. Cleary. 2000. Evaluation of the LiPA Mycobacteria assay for identification of mycobacterial species from BACTEC 12B bottles. J. Clin. Microbiol. 38:1915-1919.
14. Oliveira, R. S., M. P. Sircili, S. Y. M. Ueki, M. A. S. Telles, B. Schnabel, M. R. S. Briones, and S. C. Leao. 2000. PCR-restriction enzyme analysis of a bone marrow isolate from a human immunodeficiency virus-positive patient discloses polyclonal infection with two Mycobacterium avium strains. J. Clin. Microbiol. 38:4643-4645.
15. Picardeau, M., G. Prod'hom, L. Raskine, M. P. LePennec, and V. Vincent. 1997. Genotypic differentiation of five subspecies of Mycobacterium kansasii. J. Clin. Microbiol. 35:25-32.
16. Plikaytis, B. D., B. B. Plikaytis, and T. M. Shinnick. 1992. Computer-assisted pattern recognition model for the identification of slowly growing mycobacteria including Mycobacterium tuberculosis. J. Gen. Microbiol. 138:2265-2273.
17. Rastogi, N., K. S. Goh, and M. Berchel. 1999. Species-specific identification of Mycobacterium leprae by PCR-restriction fragment length polymorphism analysis of the hsp65 gene. J. Clin. Microbiol. 37:2016-2019.
18. Santos, A. R., A. B. De Miranda, L. M. Lima, P. N. Suffys, and W. M. Degrave. 1992. Method for high yield preparation in large and small scale of nucleic acids from mycobacteria. J. Microbiol. Methods 28:1236-1243.
19. Scarparo, C., P. Piccoli, A. Rigon, G. Ruggiero, D. Nista, and C. Piersimoni. 2001. Direct identification of mycobacteria from MB/BacT Alert 3D bottles: Comparative evaluation of two commercial probe assays. J. Clin. Microbiol. 39:3222-3227.
20. Springer, B., L. Stockman, K. Teschner, G. D. Roberts, and E. C. Bottger. 1996. Two-laboratory collaborative study on identification of mycobacteria: Molecular versus phenotypic methods. J. Clin. Microbiol. 34:296-303.
21. Springer, B., W.-K. Wu, T. Bodmer, G. Haase, G. E. Pfyffer, R. M. Kroppenstedt, K.-H. Schroder, S. Emler, J. O. Kilburn, P. Kirschner, A. Telenti, M. B. Coyle, and E. C. Bottger. 1996. Isolation and characterization of a unique group of slowly growing mycobacteria: Description of Mycobacterium lentiflavum sp. nov. J. Clin. Microbiol. 34:1100-1107.
22. Steingrube, V. A., J. L. Gibson, B. A. Brown, Y. Zhang, R. W. Wilson, M. Rajagopalan, and R. J. Wallace, Jr. 1995. PCR amplification and restriction endonuclease analysis of a 65-kilodalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria. J. Clin. Microbiol. 33:149-153.
23. Taylor, T. B., C. Patterson, Y. Hale, and W. W. Safranek. 1997. Routine use of PCR-restriction fragment length polymorphism analysis for identification of mycobacteria in liquid media. J. Clin. Microbiol. 35:79-85.
24. Telenti, A., F. Marchesi, M. Balz, F. Bally, E. Bottger, and T. Bodmer. 1993. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J. Clin. Microbiol. 31:175-178.
25. Wilson, R. W., V. A. Steingrube, B. A. Brown, and R. J. Wallace, Jr. 1998. Clinical application of the PCR-restriction enzyme pattern analysis for rapid identification of aerobic Actinomyces isolates. J. Clin. Microbiol. 36:148-152.