Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects

Plant Molecular Biology

Volumn 50, Issue 4-5, 2002, Pages 587-597

  Henriques, Rossana ^a,b   Jásik, Ján ^b,c   Klein, Markus ^d   Martinoia, Enrico ^d   Feller, Urs ^e   Schell, Jeff ^b   Pais, Maria S ^a   Koncz, Csaba ^b  

^a UNIVERSITY OF LISBON   (Portugal)

^b MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH   (Germany)

^c Physics and Informatics   (Slovakia)

^d UNIVERSITY OF NEUCHÂTEL   (Switzerland)

^e UNIVERSITY OF BERN   (Switzerland)

Author keywords

Arabidopsis; Insertion mutagenesis; IRT; Metal transporter; ZIP

Indexed keywords

ENZYME INHIBITION; GENES; IRON; SOILS; YEAST;

METAL TRANSPORTER;

PLANTS (BOTANY);

ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; CATION TRANSPORT PROTEINS; CELL DIFFERENTIATION; DNA, BACTERIAL; IRON; MUTAGENESIS, INSERTIONAL; MUTATION; PLANT ROOTS; PLANT SHOOTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, PLANT; ZINC;

ARABIDOPSIS; EMBRYOPHYTA; EUKARYOTA; INSERTION SEQUENCES;

EID: 0036836922     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1019942200164     Document Type: Article

References (43)

1. Askwith, C., Eide. D., Van Ho, A., Bernard. P.S., Li, L., Davis-Kaplan, S., Sipe, D.M. and Kaplan, J. 1994. The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. Cell 76: 403-410.
2. Bechtold, N., Ellis, J. and Pelletier, G. 1993. In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. Paris, Life Sciences, 316: 1194-1199.
3. Blaiseau, P.L., Lesuisse. E. and Camadro, J.M. 2001. Aft2p, a novel iron-regulated transcription activator that modulates, with Aft 1p, intracellular iron use and resistance to oxidative stress in yeast. J. Biol. Chem. 276: 34221-34226.
4. Briat, J.-F., Fobis-Loisy, I., Grignon, N., Lobréaux, S., Pascal, N., Savino, G., Thoiron, S., von Wirén, N. and Van Wuytswinkel, O. 1995. Cellular and molecular aspects of iron metabolism in plants. Biol. Cell 84: 69-81.
5. Briat, J.-F. and Lobréaux, S. 1997. Iron transport and storage in plants. Trends Plant Sci. 2: 187-193.
6. Clough, S.J. and Bent, A.F. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16: 735-743.
7. Cohen, C.K., Fox, T.C., Garvin, D.F. and Kochian, L.V. 1998. The role of iron-deficiency stress responses in stimulating heavy-metal transport in plants. Plant Physiol. 116: 1063-1072.
8. Curie, C., Alonso, J.M., Le Jean, M., Ecker, J.R. and Briat, J.F. 2000. Involvement of NRAMP1 from Arabidopsis thaliana in iron transport. Biochem J. 347: 749-755.
9. Curie, C., Panaviene, Z., Loulergue, C., Dellaporta, S.L., Briat, J.-F. and Walker, E.L. 2001. Maize yellow stripe 1 encodes a membrane protein directly involved in Fe(III) uptake. Nature 409: 346-349.
10. +-ATPase activity. J. Exp. Bot. 51: 695-701.
11. Dix, D.R., Bridgham, J.T., Broderius, M.A., Byersdorfer, C.A. and Eide, D.J. 1994. The FET4 gene encodes the low affinity Fe(II) transport protein of Saccharomyces cerevisiae. J. Biol. Chem. 269: 26092-26099.
12. Eide, D., Broderius, M., Fett, J. and Guerinot, M.L. 1996. A novel iron-regulated metal transporter from plants identified by functional expression in yeast. Proc. Natl. Acad. Sci. USA 93: 5624.5628.
13. Grotz, N., Fox, T., Connolly, E., Park, W., Guerinot, M.L. and Eide, D. 1998. Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. Proc. Natl. Acad. Sci. USA 95: 7220-7224.
14. Guerinot, M.L. 2000. The ZIP family of metal transporters. Biochem. Biophys. Acta 1465: 190-198.
15. Koncz, C., Martini, N., Mayerhofer, N., Koncz-Kálmán, Z., Körber, H., Rédei, G.P. and Schell, J. 1989. High-frequency T-DNA-mediated gene tagging in plants. Proc. Natl. Acad. Sci. USA 86: 8467-8471.
16. Koncz, C., Mayerhofer, R., Koncz-Kálmán, Z., Nawrath, C., Reiss, B., Rédei, G.P. and Schell, J. 1990. Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana. EMBO J. 9: 1337-1346.
17. Koncz, C., Chua, N.-H. and Schell, J. 1992. Methods in Arabidopsis Research. World Scientific, Singapore
18. Koncz, C., Martini, N., Szabados, L., Hrouda, M., Bachmair, A. and Schell, J. 1994. Specialized vectors for gene tagging and expression studies. In Gelvin, S.B. and Schilperoort, R.A. (Eds) Plant Molecular Biology Manual. Kluwer Academic Press, Dordrecht, B2: pp. 1-22.
19. Korshunova, Y.O., Eide, D. Clark, G.W., Guerinot, M.L. and Pakrasi, H.B. 1999. The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate specificity. Plant Mol. Biol. 40: 37-44.
20. Krysan, P.J., Young, J.C. and Sussman, M.R. (1999) T-DNA as an insertional mutagen in Arabidopsis. Plant Cell 12: 2283-2290.
21. Landsberg, E.C. 1994. Transfer cell formation in sugar beet roots induced by latent Fe deficiency. Plant Soil 165: 197-205.
22. Landsberg, E.C. 1996. Hormonal regulation of iron-stress response in sunflower roots: a morphological and cytological investigation. Protoplasma 194: 60-80.
23. Lyons, T.J., Gasch, A.P., Gaither, A.L., Botstein, D., Brown, P.O. and Eide, D.J. 2000. Genome-wide characterization of the Zap 1p zinc-responsive regulon in yeast. Proc. Natl. Acad. Sci. USA 97: 7957-7962.
24. Mäser, P., Thomine, S., Schroeder, J.I., Ward, J.M., Hirschi, K., Sze, H., Talke, I.N., Amtmann, A., Maathuis, F.J.M., Sanders, D., Harper, J.F., Tchieu, J., Gribskov, M., Persans, M.W., Salt, D.E., Kim, S.A. and Guerinot, M.L. 2001. Phylogenetic relationships within cation transporter familles of Arabidopsis. Plant Physiol. 126: 1646-1667.
25. Mori, S. 1999. Iron acquisition in plants. Curr. Opin. Plant Biol. 2: 250-253.
26. Németh, K., Salchert, K., Putnoky, P., Bhalerao, R., Koncz-Kálmán, Z., Stankovic-Stangeland, B., Bakó, L., Mathur, J., Ökrész, L., Stabel, S., Geigenberger, P., Stitt, M., Rédei, G.P., Schell, J. and Koncz, C. 1998. Control of glucose and hormone responses by PRL1, a nuclear WD-protein, in Arabidopsis. Genes Devel. 12: 3059-3073.
27. Reynolds, E.S. 1963. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell. Biol. 17: 208-212.
28. Robinson, N.J., Procter, C.M., Connolly, E.L. and Guerinot, M.L. 1999. A ferric-chelate reductase for iron uptake from soils. Nature 397: 694-697.
29. Rogers, S.O. and Bendich, A.J., 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5: 69-76.
30. Rogers, E.E., Eide, D.J. and Guerinot, M.L. 2000. Altered selectivity in an Arabidopsis metal transporter. Proc. Natl. Acad. Sci. USA 97: 12356-12360.
31. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
32. Schmidt, W. and Bartels, M. 1996. Formation of root epidermal transfer cells in Plantago. Plant Physiol. 110: 217-225.
33. Schmidt, W., Tittel, J. and Schikora, A. 2000. Role of hormones in the induction of iron deficiency responses in Arabidopsis roots. Plant Physiol. 122: 1109-1118.
34. Shojima, S., Nishizawa, N.K., Fushiya, S., Nozoe, S., Irifune, T. and Mori, S. 1990. Biosynthesis of phytosiderophores. In vitro biosynthesis of 2′-deoxymugineic acid from L-methionine and nicotianamine. Plant Physiol. 93: 1497-1503.
35. Spurr, A.R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruc. Res. 26: 31-43.
36. Takahashi, M., Nakanishi, H., Kawasaki, S., Nishizawa, N.K. and Mori, S. 2001. Enhanced tolerance of rice to low iron availability in alkaline soils using barley nicotianamine aminotransferase genes. Nature Biotech. 19: 466-469.
37. Thimm, O., Essigmann, B., Kloska, S., Altmann, T. and Buckhout, T.J. 2001. Response of Arabidopsis to iron deficiency stress as revealed by microarray analysis. Plant Physiol. 127: 1030-1043.
38. Thomine, S., Wang, R., Ward, J.M., Crawford, N.M. and Schroeder, J.I. 2000. Cadmium and iron transport by members of a plant metal transporter family in Arabidopsis with homology to Nramp genes. Proc. Natl. Acad. Sci. USA 97: 4991-4996.
39. Vert, G., Briat, J.-F. and Curie, C. 2001. Arabidopsis IRT2 gene encodes a root-periphery iron transporter. Plant J. 26: 181-189.
40. Von Wirén, N., Klair, S., Bansal, S., Briat, J.-F., Khodr, H., Shioiri, T., Leigh, R.A. and Hider. R.C. 1999. Nicotianamine chelates both Fe III and Fe II. Implications for metal transport in plants. Plant Physiol. 119: 1107-1114.
41. Waldo, G.S., Wright, E., Whang, Z.H., Briat, J.F., Theil, E.C. and Sayers, D.E. 1995. Formation of the ferritin iron mineral occurs in plastids. Plant Physiol. 109: 797-802.
42. Yi, Y. and Guerinot, M.L. 1996. Genetic evidence that induction of root Fe(III) chelate reductase activity is necessary for iron uptake under iron deficiency. Plant J. 10: 835-844.
43. Young, J.C., Krysan, P.J. and Sussman, M.R. 2001. Efficient screening of Arabidopsis T-DNA insertion lines using degenerate primers. Plant Physiol. 125: 513-518.