Immunocytological localization of two enzymes involved in berberine biosynthesis

Planta

Volumn 216, Issue 1, 2002, Pages 57-63

  Bock, Armin ^b   Wanner, Gerhard ^b   Zenk, Meinhart H ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Alkaloid compartmentation; Berberis; Enzyme compartmentation; Isoquinoline alkaloid; Papaver

Indexed keywords

ANTIBODIES; BIOSYNTHESIS; ENZYME KINETICS; MICROSCOPIC EXAMINATION;

IMMUNOGOLD TECHNIQUES;

PLANTS (BOTANY);

BERBERIS; PAPAVER; PAPAVER SOMNIFERUM;

ALKALOID; BENZYLISOQUINOLINE DERIVATIVE; BERBERINE; BERBERINE DERIVATIVE; CANADINE; DISCRETAMINE; DRUG DERIVATIVE; ISOQUINOLINE; ISOQUINOLINE DERIVATIVE; N DEMETHYLASE; OXIDOREDUCTASE; RETICULINE; RETICULINE OXIDASE; TETRAHYDROPROTOBERBERINE OXIDASE;

ARTICLE; BERBERIS; CELL CULTURE; CELL VACUOLE; CYTOLOGY; ESCHSCHOLTZIA; IMMUNOELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; METABOLISM; PAPAVER; PLANT; ULTRASTRUCTURE;

ALKALOIDS; BENZYLISOQUINOLINES; BERBERINE; BERBERINE ALKALOIDS; BERBERIS; CELLS, CULTURED; ESCHSCHOLZIA; IMMUNOHISTOCHEMISTRY; ISOQUINOLINES; MICROSCOPY, IMMUNOELECTRON; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; OXIDOREDUCTASES, N-DEMETHYLATING; PAPAVER; PLANTS; VACUOLES;

EID: 0036834176     PISSN: 00320935     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00425-002-0867-5     Document Type: Article

References (20)

1. Amann M, Wanner G, Zenk MH (1986) Intracellular compartmentation of two enzymes of berberine biosynthesis in plant cell cultures. Planta 167:310-320
2. Amann M, Nagakura N, Zenk MH (1988) Purification and properties of (S)-tetrahydroprotoberberine oxidase from suspension-cultured cells of Berberis wilsoniae. Eur J Biochem 175:17-25
3. Beerhues L, Robenek H, Wiermann R (1988) Chalcone synthases from spinach (Spinacia oleracea L.). II. Immunofluorescence and immunogold localization. Planta 173:544-553
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
5. Cline SD, Coscia CJ (1988) Stimulation of sanguinarine production by combined fungal elicitation and hormonal deprivation in cell suspension cultures of Papaver bracteatum. Plant Physiol 86:161-165
6. Cline SD, Coscia JC (1989) Ultrastructural changes associated with the accumulation and secretion of sanguinarine in Papaver bracteatum suspension cultures treated with fungal elicitor. Planta 178:303-314
7. Deliu C, Craciun C, Craciun V, Tamas M (1994) Ultrastructural and biochemical study of Berberis parvijolia root meristem and cell cultures. Plant Sci 96:143-150
8. Dittrich H, Kutchan TM (1991) Molecular cloning, expression and induction of berberine bridge enzyme, an enzyme essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Proc Natl Acad Sci USA 88:9969-9973
9. Hahn MG, Albersheim P (1978) Host-pathogen interactions. XIV. Isolation and partial characterization of an elicitor from yeast extract. Plant Physiol 62:107-111
10. Kocourek J, Ballou CE (1969) Method for fingerprinting yeast cell wall mannans. J Bacteriol 100:1175-1181
11. Kutchan TM, Bock A, Dittrich H (1994) Heterologous expression of the plant proteins strictosidine synthase and berberine bridge enzyme in insect cell culture. Phytochemistry 35:353-360
12. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100-127
13. Rush MD, Kutchan TM, Coscia CJ (1985) Correlation of the appearance of morphinan alkaloids and laticifer cells in germinating Papaver bracteatum seedlings. Plant Cell Rep 4:237-240
14. Schöpker H, Kneisel M, Beerhues L, Robenek H, Wiermann R (1995) Phenylalanine ammonia-lyase and chalcone synthase in glands of Primula kewensis (W. Wats): immunofluorescence and immunogold localization. Planta 196:712-719
15. Schumacher HM, Gundlach H, Fiedler F, Zenk MH (1987) Eliciration of benzophenanthridine alkaloid synthesis in Eschscholzia cell cultures. Plant Cell Rep 6:410-413
16. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31-43
17. Steffens P, Nagakura N, Zenk MH (1985) Purification and Characterization of the berberine bridge enzyme from Berberis beaniana cell cultures. Phytochemistry 24:2577-2583
18. Von Heijne G (1986) A new method for predicting signal sequence cleavage sites. Eur J Biochem 135:123-126
19. Yamamoto H, Nakagawa K, Fukui H, Tabata M (1986) Cytological changes associated with alkaloid production in cultured cells of Coptis japonica and Thalictrum minus. Plant Cell Rep 5:65-68
20. Zenk MH (1995) Chasing the enzymes of alkaloid biosynthesis. In: Golding BT, Griffin RI, Maskill H (eds). Organic reactivity: physical and biological aspects. The Royal Society of Chemistry, Newcastle upon Tyne, pp 89-109