Identification and characterization of the peptidic component of the immunomodulatory glycoconjugate Immunoferon®

Methods and Findings in Experimental and Clinical Pharmacology

Volumn 24, Issue 8, 2002, Pages 471-480

  Varela, J ^a   Navarro Pico, M L ^a   Guerrero, A ^b   Garcia F ^b   Gimenez Gallego G ^a   Pivel, Juan Pablo ^b  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b Industrial Farmaceutica Cantabria SA   (Spain)

Author keywords

AM3; Immunomodulator; Inmunoferon; Protein; Ricinus communis

Indexed keywords

GLYCOCONJUGATE; IMMUNOFERON; IMMUNOMODULATING AGENT; PEPSIN A; POLYPEPTIDE; POLYSACCHARIDE; PROTEIN AM55; PROTEIN RICC3; THERMOLYSIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; DISULFIDE BOND; GEL FILTRATION CHROMATOGRAPHY; IMMUNOMODULATION; PH; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN ISOLATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; RICINUS COMMUNIS;

ADJUVANTS, IMMUNOLOGIC; ALBUMINS; CALCIUM PHOSPHATES; DATABASES, PROTEIN; DIMERIZATION; GLYCOCONJUGATES; GLYCOPEPTIDES; MOLECULAR WEIGHT; PEPTIDES; PLANT PROTEINS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; RICINUS; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; TRYPSIN;

EID: 0036814001     PISSN: 03790355     EISSN: None     Source Type: Journal    
DOI: 10.1358/mf.2002.24.8.705066     Document Type: Article

References (17)

1. Brieva. A., Garcia-Villarubia, V., Guerrero. A., Pivel, J.P., Giménez-Gallego, G., Matji-Tuduri, J.A. Glicoconjugados de polipéptidos farmacológicamente activos. Patent P9900408 1999.
2. Moya, P., Baixeras, E., Barasoain, I. et al. Inmunoferon® (AM3) enhances the activities of early-type interferon inducers and natural killer cells, Immunopharmacol Immunotoxicol 1987, 9: 243-56.
3. Brieva, A., Guerrero, A., Alonso-Lebrero. J., Pivel. J. Inmunoferon®, a glycoconjugate of natural origin, inhibits LPS-induced TNF-alpha production and the response to endotoxemia. Intl Immunopharmacol 2001, 1: 1979-87.
4. Sánchez, L., Peña, E., Civantos, A. et al. AM3, an adjuvant to hepatitis B revaccination in non-responder healthy persons. J Hepatol 1995, 22: 119-21.
5. Brieva. A., Guerrero. A., Alonso-Lebrero, J., Pivel, J. Inmunoferon® a glycoconjugate of natural origin, regulates the liver response to inflammation and inhibits TNF-α production by an HPA axis-dependent mechanism. Intl Immunopharmacol 2002. 2: 807-13.
6. Guerrero, A., Brieva. A., Pivel, J.P, A new method for radioiodination of polysaccharides and its use in biodistribution studies of an immunomodulating glycoconjugate (Inmunoferon) Exp Clin Pharmacol 2000. 22: 621-5.
7. Wilkins, M.R., Gasteiger, E., Bairoch, A. et al. Protein Identification and Analysis Tools in the ExPASy Server. Humana Press: New Jersey 1996.
8. Wilkins, M.R., Ou, K., Appel, R.D. et al. Rapid protein identification using N-terminal "sequence tag" and amino acid analysis. Biochem Biophys Res Commun 1996, 221: 609-13.
9. Wilkins, M.R., Pasquali, C., Appel, R.D. et al. From proteins to proteomes: Large scale protein identification by two-dimensional electrophoresis and amino acid analysis. Biotechnology 1996. 14: 61-5.
10. Stults, J.T Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Curr Opin Struct Biol 1995, 5: 691-8.
11. Zaluzec, E.J., Gage, D.A., Watson. J.T. Matrix-assisted laser desorption ionization mass spectrometry: Applications in peptide and protein charaeterization. Protein Expr Purif 1995, 6: 109-23.
12. Kaufmann, R. Matrix-assisted laser desorption ionization (MALDI) mass spectrometry: A novel analytical tool in molecular biology and biotechnology. J Biotechnol 1995, 41: 155-75.
13. Perczel, A., Park, K., Fasman, G.D. Analysis of the circular dichroism spectrum of proteins using the convex constrctint algorithm: A practical guide. Anal Biochem 1992, 203: 83-93.
14. Bashir, M.,Hubatsch, I., Leinenbach, H., Zeppezauer., Z., Panzani. R., Hussein, I. RicC1 and RicC3. The allergenic 2S albumin storage proteins of Ricinus communis: Complete primary structures and phylogenetic relationships. Intl Arch Allergy Immunol 1998, 115: 73-82.
15. Irwin, S.D., Keen, J.N., Findlay, J.B., Lord, J.M. The Ricinus communis 2S albumin precursor: A single preproprotein may be processed into two different heterodimeric storage proteins. Mol Gen Genet 1990, 22: 400-8.
16. Irwin, SD., Lord, J.M. Nucleotide sequence of a Ricinus communis 2S albumin precursor gene. Nucleid Acids Res 1990, 18: 5890.
17. Da Silva, J.G., Machado, O.L., Izumi. C. et al. Amino acid sequence of a new 2S albumin from Ricinus communis which is part of a 29-kD precursor protein. Arch Biochem Biophys 1996, 336: 10-8.